全文获取类型
收费全文 | 251篇 |
免费 | 16篇 |
出版年
2019年 | 2篇 |
2017年 | 5篇 |
2016年 | 7篇 |
2015年 | 11篇 |
2014年 | 10篇 |
2013年 | 8篇 |
2012年 | 8篇 |
2011年 | 6篇 |
2010年 | 6篇 |
2009年 | 4篇 |
2007年 | 5篇 |
2006年 | 3篇 |
2005年 | 8篇 |
2001年 | 4篇 |
2000年 | 7篇 |
1999年 | 6篇 |
1998年 | 10篇 |
1997年 | 7篇 |
1996年 | 6篇 |
1995年 | 6篇 |
1994年 | 6篇 |
1992年 | 3篇 |
1990年 | 4篇 |
1988年 | 2篇 |
1987年 | 5篇 |
1986年 | 2篇 |
1982年 | 4篇 |
1981年 | 2篇 |
1980年 | 6篇 |
1978年 | 3篇 |
1977年 | 4篇 |
1975年 | 2篇 |
1973年 | 5篇 |
1972年 | 5篇 |
1961年 | 1篇 |
1960年 | 1篇 |
1959年 | 4篇 |
1958年 | 5篇 |
1957年 | 4篇 |
1956年 | 7篇 |
1955年 | 3篇 |
1954年 | 8篇 |
1953年 | 2篇 |
1952年 | 5篇 |
1951年 | 10篇 |
1950年 | 7篇 |
1949年 | 4篇 |
1948年 | 5篇 |
1946年 | 3篇 |
1934年 | 1篇 |
排序方式: 共有267条查询结果,搜索用时 31 毫秒
101.
Energy reserves and metabolic expenditures of monarch butterflies overwintering in southern California 总被引:1,自引:0,他引:1
Abstract. 1. Energetic expenditure and predicted requirements for overwintering metabolism were determined for monarch butterflies ( Danaus plexippus L.) in southern California.
2. Fat content of butterflies declined steadily from a maximum of 71% lean dry weight in late November to a minimum of 36% lean dry weight in late January. The energy expenditure measured by fat depletion over a 61 day period from 24 November to 25 January was 26.05 joules per day.
3. Butterflies were generally the same temperature as the air at any time they were inactive, whether they were part of a large cluster or roosting solitarily.
4. Oxygen consumption of butterflies increased in a curvilinear fashion with increasing air temperature. Thus, the predicted metabolic requirements for an inactive butterfly during their quiescent period from late November to late January was 30.32 joules per day.
5. In contrast to the steady depletion of fat reserves during their quiescent period in December and January, low and stable fat levels of butterflies in late February coincide with high levels of flight activity, mating and emigration of females from the colony.
6. Progressive tightening of the energy balance due to increasing nocturnal temperatures from northern to southern California coastal areas may limit the southern distribution and duration of overwintering aggregations. 相似文献
2. Fat content of butterflies declined steadily from a maximum of 71% lean dry weight in late November to a minimum of 36% lean dry weight in late January. The energy expenditure measured by fat depletion over a 61 day period from 24 November to 25 January was 26.05 joules per day.
3. Butterflies were generally the same temperature as the air at any time they were inactive, whether they were part of a large cluster or roosting solitarily.
4. Oxygen consumption of butterflies increased in a curvilinear fashion with increasing air temperature. Thus, the predicted metabolic requirements for an inactive butterfly during their quiescent period from late November to late January was 30.32 joules per day.
5. In contrast to the steady depletion of fat reserves during their quiescent period in December and January, low and stable fat levels of butterflies in late February coincide with high levels of flight activity, mating and emigration of females from the colony.
6. Progressive tightening of the energy balance due to increasing nocturnal temperatures from northern to southern California coastal areas may limit the southern distribution and duration of overwintering aggregations. 相似文献
102.
103.
A review of Schizopera Sars indicates that four species are so much more primitive in the antenna and leg setation that they should be removed to a new genus, Eoschizopera, which can be considered as a direct and immediate ancestor of Schizopera. A further, new, species is described in this new genus. The relationships within this branch of the family Diosaccidae are discussed and the scheme of family evolution proposed by Lang is modified to include Eoschizopera and other genera not considered by him (Goffinella, Protopsammotopa, Psammotopa, Actopsyllus, Balucopsylla, Schizoperoides). Actopsyllus hartmannorum Kunz is removed to a further new genus- Helmutkunzia. 相似文献
104.
JEREMY J. HEATH BRENDA WELLS DON CIPOLLINI JOHN O. STIREMAN III 《Ecological Entomology》2013,38(1):11-22
1. Adaptive divergence in sympatry is supposed to be inhibited by the homogenizing role of gene flow. However, studies continue to uncover examples of sympatric divergence. In this study, two divergent phenotypes in a complex of four syntopic gall midge morphotypes [nominally Asteromyia carbonifera Osten Saken, Diptera: Cecidomyiidae: Alycaulini] are characterised. The first is a behavioural phenotype governing within‐host tissue preference and the second is a trait governing accessory‐gland carotenoid quality and quantity. 2. One gall morphotype (crescents) lay most of their eggs on mature tissue whereas the other three gall morphotypes oviposit only on young emerging leaves. Ecological maintenance of this divergent trait appears to be driven by enemy‐reduced space. That is, nearly 40% of the crescent morphotype galls that develop high on the plant are attacked by the egg parasitoid Platygaster solidaginis Ashmed, whereas those low on the plant are relatively protected. 3. All morphotypes contain carotenoids in their accessory glands, but the quality and quantity of these pigments differs significantly between the morphotypes and is positively associated with the probability of parasitism by P. solidaginis. 4. Larval salivary glands also contain carotenoids and the plant hormone abscisic acid, which in plants is synthesized from carotenoid precursors and is involved in regulating plant defences. This hormone may facilitate gall development and influence gall morphology. 5. Ecological fitness trade‐offs between carotenoids, parasitoid attack, and plant resistance may be fostering adaptive divergence in ovipositional phenotypes and sympatric speciation in this complex of gall midge morphotypes. 相似文献
105.
Cultured callus tissues derived from endosperm of Oxalis disparare shown to contain virescent amyloplasts. In darkness, proplastidsdevelop into typical amyloplasts, starch being deposited assingle or multiple grains. In light, amyloplasts are transformedinto chloroplasts. Thylakoid formation begins in spaces aroundand between existing starch grains. As thylakoids are assembledinto grana, starch slowly disappears; the plastids increasein size and the photosynthetic apparatus enlarges to fill thewhole of the plastid. Slight carotenoid synthesis takes placeas amyloplasts are laid down, but there is no chlorophyll synthesis.All pigments accumulate rapidly during the early stages of granaldevelopment, but slowly, and at a declining rate, during thelater stages. Treatment of the tissues with auxins suppressesthe development of thylakoid membranes, but has no effect uponthe development of amyloplast membranes. The possible significanceof this observation is discussed. Greening is accompanied by a marked decline in the rates ofboth cell division and cell expansion. This is attributed inpart to the diversion of nitrogen from the normal growth channelsinto the synthesis of thylakoid proteins. 相似文献
106.
107.
E C Castro JA Diaz GomezDe Ferreyra C R De Castro N D'Acosta C M De Fenos 《Biochemical and biophysical research communications》1973,50(2):337-343
There is a higher activity of ethyl morphine N-demethylase (EM-ase) and cytochrome P-450 (P-450) reductase as well as higher P-450 content in the smooth endoplasmic reticulum (SER) than in the rough endoplasmic reticulum (RER). The extent of the irreversible binding of the14C from14CCl4 to lipids and proteins, as well as the CCl4-induced destruction of P-450 is more intense in SER than in RER while the opposite was found for glucose 6-phosphatase (G6P-ase) destruction. CCl4-induced lipid peroxidation is as intense in SER as is in RER.14C from14CCl4 gets irreversibly bound to ribosomal proteins. 相似文献
108.
Peter Thorpe Sophie Mantelin Peter JA Cock Vivian C Blok Mirela C Coke Sebastian Eves-van den Akker Elena Guzeeva Catherine J Lilley Geert Smant Adam J Reid Kathryn M Wright Peter E Urwin John T Jones 《BMC genomics》2014,15(1)
Background
The potato cyst nematode Globodera pallida has biotrophic interactions with its host. The nematode induces a feeding structure – the syncytium – which it keeps alive for the duration of the life cycle and on which it depends for all nutrients required to develop to the adult stage. Interactions of G. pallida with the host are mediated by effectors, which are produced in two sets of gland cells. These effectors suppress host defences, facilitate migration and induce the formation of the syncytium.Results
The recent completion of the G. pallida genome sequence has allowed us to identify the effector complement from this species. We identify 128 orthologues of effectors from other nematodes as well as 117 novel effector candidates. We have used in situ hybridisation to confirm gland cell expression of a subset of these effectors, demonstrating the validity of our effector identification approach. We have examined the expression profiles of all effector candidates using RNAseq; this analysis shows that the majority of effectors fall into one of three clusters of sequences showing conserved expression characteristics (invasive stage nematode only, parasitic stage only or invasive stage and adult male only). We demonstrate that further diversity in the effector pool is generated by alternative splicing. In addition, we show that effectors target a diverse range of structures in plant cells, including the peroxisome. This is the first identification of effectors from any plant pathogen that target this structure.Conclusion
This is the first genome scale search for effectors, combined to a life-cycle expression analysis, for any plant-parasitic nematode. We show that, like other phylogenetically unrelated plant pathogens, plant parasitic nematodes deploy hundreds of effectors in order to parasitise plants, with different effectors required for different phases of the infection process.Electronic supplementary material
The online version of this article (doi:10.1186/1471-2164-15-923) contains supplementary material, which is available to authorized users. 相似文献109.
Daiane D Hartwig Thaís L Oliveira Fabiana K Seixas Karine M Forster Caroline Rizzi Cláudia P Hartleben Alan JA McBride Odir A Dellagostin 《Microbial cell factories》2010,9(1):98
Background
Leptospirosis, a zoonosis caused by Leptospira spp., is recognized as an emergent infectious disease. Due to the lack of adequate diagnostic tools, vaccines are an attractive intervention strategy. Recombinant proteins produced in Escherichia coli have demonstrated promising results, albeit with variable efficacy. Pichia pastoris is an alternative host with several advantages for the production of recombinant proteins. 相似文献110.