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111.
猪源肠球菌的分离及生物特性的初报 总被引:4,自引:0,他引:4
本研究从猪粪便中分离了肠球菌两珠,经菌种鉴定两珠菌均为屡肠球菌。其生物特性的研究表明:可耐受15的胆盐和6%NaCI高盐,在pH3.0条件下可存活,对绝大多数抗生素耐药。 相似文献
112.
A commercially available assay for aldosterone that requires no extraction was found to be valid without modification for rat serum. The assay was performed in duplicate on as little as 0.5 cc of rat serum and gave linear results from 5 ng/dl to 500 ng/dl, (i.e., the physiological range of the rat). Samples above 150 ng/dl had to be diluted in this assay. The cost per sample if performed in duplicate and in batches was approximately one dollar. We found that this assay, which was developed for and is marketed for determining aldosterone concentration in human samples, offered a cost effective assay for aldosterone determination in the rat. 相似文献
113.
Biomarkers have been used by pathologists to aid the diagnosis of tumors for almost three decades. Their use has resulted in the re-evaluation and reclassification of several types of tumors. Currently, biomarkers are required to differentiate certain specific tumors with similar histologic patterns. Additional uses of biomarkers in the characterization of neoplastic processes are discussed including their use in prognosis, detecting early neoplastic processes, identifying tumor recurrence, measuring the effectiveness of various therapies (surrogate end point biomarkers), and identifying targets for novel therapies including immunotherapy and gene therapy. We propose that these newer uses of biomarkers will be just as important to pathology in the future as the uses of biomarkers in diagnosis have been over the past two decades. 相似文献
114.
Personnel in medical, veterinary or research laboratories may be exposed to a wide variety of pathogens that range from deadly to debilitating. For some of these pathogens, no treatment is available, and in other cases the treatment does not fully control the disease. It is important that personnel in laboratories that process human or microbiological specimens follow universal precautions when handling tissues, cells, or microbiological specimens owing to the increasing numbers of individuals infected with hepatitis C and HIV in the US and the possibility that an individual may be asymptomatic when a specimen is obtained. Similar precautions must be followed in laboratories that use animal tissues owing to the possibility of exposure to agents that are pathogenic in humans. Personnel with conditions associated with immunosuppression should evaluate carefully whether or not specific laboratory environments put them at increased risk of disease. We offer here some general approaches to identifying biohazards and to minimizing the potential risk of exposure. The issues discussed can be used to develop a general safety program as required by regulatory or accrediting agencies, including the Occupational Safety and Health Administration. 相似文献
115.
116.
William E. Grizzle 《Biotechnic & histochemistry》1997,72(2):59-61
The papers in this symposium were presented at the 1996 Annual Meeting of the Biological Stain Commission. The authors hope that the discussion of biomarkers in the papers included in this issue and the literature reviewed in them will be useful to diagnostic pathologists as well as to investigators studying neoplastic processes in general. 相似文献
117.
W. C. Bell R. B. Myers T. O. Hosein D. K. Oelschlager W. E. Grizzle 《Biotechnic & histochemistry》2006,78(1):11-16
The mechanisms by which androgens stimulate proliferation of prostate cancer cells are poorly understood. It has been proposed that androgen stimulation may induce the mitogen-activated protein (MAP) kinase system in prostate cancer cells and lead to cellular proliferation. We attempted to evaluate the role of the extracellular signal-regulated kinase (ERK) pathway in the stimulation by androgens of prostate cancer cell proliferation. Androgen-sensitive prostate cancer cell line (LNCaP) cells plated on sterile glass coverslips were treated with 10-8 M dihydrotestosterone (DHT) or epidermal growth factor (EGF) (10 ng/ml) for periods ranging from 1 min to 96 h. The proliferative index of the cells, evaluated by immunoperoxidase staining of cells with an antibody to Ki-67, was increased at least two-fold at all time points from 5 min to 48 h following exposure to either DHT or EGF. Immunohistochemical evaluation of ERK1/2 and pERK (activated ERK) demonstrated high levels of ERK1/2 in untreated LNCaP cells, while pERK was expressed at much lower levels. Following treatment with DHT, no change in staining intensity for either ERK1/2 or pERK was observed, while treatment with EGF resulted in no change in ERK1/2, but significantly increased cytoplasmic staining for pERK at all time points beyond 2 min. These results were confirmed by Western blot analysis of ERK1/2 and pERK expression in these cell lines following treatment with DHT or EGF. Our findings suggest that the proliferative response of prostate cancer cells to androgens, unlike the proliferative response to EGF, is not mediated by the activation of ERK1/2, and that currently undefined pathways other than those involving ERK1/2 are involved. 相似文献