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91.
92.
第三脑室注射组胺及其受体激动剂对五肽促胃液素诱导的大鼠胃酸分泌的影响 总被引:1,自引:0,他引:1
本文报道第三脑室注射组胺(0.25—2.0μg/5μl)对五肽促胃液素诱导的胃酸分泌的双重影响。雄性Wistar大鼠,重200—300g,戊巴比妥钠腹腔麻醉。用37℃生理盐水通过恒流泵进行连续胃灌流。在静脉恒速灌注五肽促胃液素(7.5μg/kg·h)的基础上,第三脑室注射组胺(0.25μg/5μl)或H_1受体激动剂2-Pyridylethylamine(PEA,10μg/5μl),10min后总酸排出量即开始减少,90min仍未恢复。组胺剂量增至1.0μg或2.0μg时,出现双重效应。部份动物(分别占73%或50%)胃酸分泌减少,另一部分动物(27%或50%)胃酸分泌增多。H_2受体激动剂dimaprit(10μg/5μl)或impromidine(0.1μg/5μl)对胃酸分泌无明显影响。苯海拉明(16μg/0.2ml或32μg/0.2ml,i.m.)预处理可分别取消组胺和PEA的抑胃酸效应。这些结果提示:脑内组胺可能参与胃酸分泌中枢调节。其抑制效应似通过H_1受体介导;双重效应的机制有待进一步研究。 相似文献
93.
94.
趾钩是鳞翅目幼虫分类上常用的特征之一。从本世纪40年代起,神冈等对家蚕幼虫腹足趾钩进行了广泛的研究,中岛(1956)对柞蚕幼虫腹足趾钩有过简短的报告。柞蚕幼虫腹足趾钩不但与地理品种分化有关,而且,由于柞蚕幼虫在野外山林中生活,柞蚕取食等活动,均靠趾钩把握柞枝,趾钩与柞蚕把握力有着密切关系。现将柞蚕幼虫腹足趾钩的形态特征扼要报告如下。 相似文献
95.
作者曾于1986年首次报道了在我国四川省汶川县卧龙地区发现的指虻属(Isshikia)一新种——汶川指虻。本文再次记述了在我国海南省发现的另一指虻属新种——海南指虻。另外同时记述在我国西藏地区发现的瘤虻属(Hybomitra)一新种——黑须瘤虻。模式标本均保存于中国科学院动物研究所。 相似文献
96.
X Tang H Tashiro T Eki Y Murakami E Soeda T Sakakura P C Watkins K Yokoyama 《Genomics》1992,14(1):185-187
Sequence-tagged sites (STSs) are short stretches of DNA that can be specifically detected by the polymerase chain reaction (PCR) and can be used to construct long-range physical maps of chromosomal DNA. These STSs can be detected by PCR assays developed by reference to data obtained from the sequencing of restriction fragment length polymorphism-DNA markers for chromosome 21, which were derived from recombinant lamba-phage and plasmid clones made from DNA of a human-hamster hybrid cell line. In this report, we describe the generation of 19 new STSs that are specific for human chromosome 21. 相似文献
97.
Effects of microsite light availability on the growth and survival of transplantedQuercus serrata Thunb. seedlings in aMiscanthus sinensis Anderss. grass canopy were investigated by a “plant's eye view” approach. Diffuse site factors, i.e., the fractional transmissions
of diffuse photosynthetic photon flux density, were estimated at 15 cm aboveground in 108 microsites where the seedlings grew.
Microsite diffuse site factors were significantly different between surviving and dead seedlings during the experiment period
from April to October (F[1,14]=10.9, P<0.01). Relative growth rate of dry weight for individual seedlings positively depended on the diffuse site factors
(r2=0.482, P<0.001 in May; r2=0.312, P<0.001 in October). Only 16 seedlings produced their second stem flush within the grass canopy. The ratio of height
to dry weight of the second stem flush was significantly higher for the seedlings grew in shady microsites than for those
in less shady microsites (r2=0,471, P<0.01 in May). This study suggests that the microsite heterogeneity of light availability is one of the important
factors affecting the establishment of tree seedlings in patchy grasslands. 相似文献
98.
Several CD4 domains can play a role in human immunodeficiency virus infection in cells. 总被引:5,自引:4,他引:1
L Poulin L A Evans S B Tang A Barboza H Legg D R Littman J A Levy 《Journal of virology》1991,65(9):4893-4901
The human immunodefiency virus (HIV) uses the human CD4 glycoprotein as a receptor for infection of susceptible cells. Cells expressing a series of mutated forms of the CD4 gene have shown a variability in their ability to support replication of three HIV type 1 (HIV-1) and three HIV-2 strains. Moreover, when different stages of virus production were examined by a variety of assays, a consistent delay was observed in all cell lines containing CD4 mutants compared with those with intact full-length CD4. Cells expressing the CD4.415 mutant (modified at the serine 415 corresponding to a phosphorylation site of the cytoplasmic domain) showed only a minimal effect on virus replication. Cells expressing CD4.403 and CD4.401 mutants (lacking the whole cytoplasmic domain) manifested a moderate delay in production of virus progeny. The most substantial effect on HIV replication was observed in cells expressing a chimeric hybrid containing sequences corresponding to the first 177 residues of the N-terminal CD4 fused to CD8 sequences encoding the hinge, transmembrane, and cytoplasmic domains of the human CD8. Furthermore, in a cell-to-cell contact assay, fusion was absent when the CD4 proximal membrane domain was replaced by the CD8 counterpart. In addition, a strong correlation between the down-modulation of the surface CD4 and HIV expression was observed. These observations suggest that in addition to the known binding region, other domains of CD4 could play an important role in regulating HIV entry of cells. 相似文献
99.
Biologic markers in ethylene oxide-exposed workers and controls 总被引:2,自引:0,他引:2
J Mayer D Warburton A M Jeffrey R Pero S Walles L Andrews M Toor L Latriano L Wazneh D Tang 《Mutation research》1991,248(1):163-176
Ethylene oxide (EtO) is an alkylating agent and a model direct-acting mutagen and carcinogen. This study has evaluated a panel of biologic markers including EtO-hemoglobin adducts (EtO-Hb), sister-chromatid exchanges (SCEs), micronuclei, chromosomal aberrations (CAs), DNA single-strand breaks (SSB) and an index of DNA repair (ratio of UDS to NA-AAF-DNA binding) in the peripheral blood cells of 34 workers at a sterilization unit of a large university hospital and 23 controls working in the university library. Comprehensive environmental histories were obtained on each subject including detailed occupational and smoking histories. Industrial hygiene data obtained prior to the study and personal monitoring during the 8 years preceding the study showed that workers were subject to low-level exposure near or below the current Occupational Safety and Health Administration (OSHA) standard of 1 ppm (TWA). Personal monitoring data obtained during 2 weeks prior to blood sampling were uniformly less than 0.3 ppm (TWA). After adjusting for smoking, EtO workplace exposure was significantly (p less than 0.001) associated with EtO-Hb (a carcinogen-protein adduct) and 2 measures of SCEs [the average number of SCEs/cell (SCE50) and the number of high frequency cells (SCEHFC)]. There was an apparent suppression of DNA repair capacity in EtO-exposed individuals as measured by the DNA repair index; i.e., the ratio of unscheduled DNA synthesis (UDS) and NA-AAF-DNA binding (p less than 0.01). No association of DNA repair index with smoking was found. Another important finding of this study is the highly significant correlation between EtO-Hb adduct levels and SCEHFC (p less than 0.01) and SCEs (p less than 0.02) which provides evidence of a direct link between a marker of biologically effective dose and markers of genotoxic response. In contrast, micronuclei, CAs and SSBs were not significantly elevated in the workers. The activity of the u-isoenzyme of glutathione-S-transferase (GT) was measured as a possible genetic marker of susceptibility and a modulator of biomarker formation. However, possibly because of confounding by age, no significant relationships were found between GT and any of the exposure-related markers by ANOVA or among other independent variables by regression. This study demonstrates significant effects of low-level EtO exposure, independent of smoking history, near or below 1 ppm on multiple biomarkers and suggests that the current OSHA standard may not be adequately protective. Previously described effects of smoking on EtO-Hb adducts, SCEs and SCEHFC were also seen in this study.(ABSTRACT TRUNCATED AT 400 WORDS) 相似文献
100.
Helen L. Henry Chhanda Dutta Noreen Cunningham Raymond Blanchard Robert Penny Chilung Tang Gail Marchetto Shu-Yin Chou 《The Journal of steroid biochemistry and molecular biology》1992,41(3-8)
The synthesis of 1,25(OH)2D3 is a critical control point in the regulation of calcium metabolism, and possibly in the growth and differentiation of a number of cell types. This paper reviews our current understanding of the regulation of this process at the cellular and molecular levels, with the emphasis on the mechanisms of feedback control 1,25(OH)2D3 itself, control of parathyroid hormone, the roles of cyclic AMP dependent protein kinase and protein kinase C, and the interaction between the various intracellular regulators of 1,25(OH)2D3 production. 相似文献