全文获取类型
收费全文 | 1454286篇 |
免费 | 136187篇 |
国内免费 | 1522篇 |
专业分类
1591995篇 |
出版年
2021年 | 17881篇 |
2019年 | 16143篇 |
2018年 | 17817篇 |
2017年 | 16549篇 |
2016年 | 28053篇 |
2015年 | 42413篇 |
2014年 | 50509篇 |
2013年 | 76692篇 |
2012年 | 38602篇 |
2011年 | 27383篇 |
2010年 | 43984篇 |
2009年 | 45249篇 |
2008年 | 26059篇 |
2007年 | 24075篇 |
2006年 | 29299篇 |
2005年 | 30477篇 |
2004年 | 29516篇 |
2003年 | 27333篇 |
2002年 | 25293篇 |
2001年 | 41579篇 |
2000年 | 39232篇 |
1999年 | 37162篇 |
1998年 | 26237篇 |
1997年 | 26139篇 |
1996年 | 25593篇 |
1995年 | 23630篇 |
1994年 | 23481篇 |
1993年 | 22660篇 |
1992年 | 32087篇 |
1991年 | 30502篇 |
1990年 | 28782篇 |
1989年 | 29530篇 |
1988年 | 27367篇 |
1987年 | 25999篇 |
1986年 | 24404篇 |
1985年 | 26158篇 |
1984年 | 25083篇 |
1983年 | 22240篇 |
1982年 | 21847篇 |
1981年 | 20919篇 |
1980年 | 19513篇 |
1979年 | 21523篇 |
1978年 | 19634篇 |
1977年 | 18659篇 |
1976年 | 17756篇 |
1975年 | 17543篇 |
1974年 | 18239篇 |
1973年 | 18495篇 |
1972年 | 15826篇 |
1971年 | 14545篇 |
排序方式: 共有10000条查询结果,搜索用时 8 毫秒
991.
Murine Cytomegalovirus m02 Gene Family Protects against Natural Killer Cell-Mediated Immune Surveillance 下载免费PDF全文
Sofia A. Oliveira Se-Ho Park Peter Lee Albert Bendelac Thomas E. Shenk 《Journal of virology》2002,76(2):885-894
The murine cytomegalovirus m02 gene family encodes putative type I membrane glycoproteins named m02 through m16. A subset of these genes were fused to an epitope tag and cloned into an expression vector. In transfected and murine cytomegalovirus-infected cells, m02, m04, m05, m06, m07, m09, m10, and m12 localized to cytoplasmic structures near the nucleus, whereas m08 and m13 localized to a filamentous structure surrounding the nucleus. Substitution mutants lacking the m02 gene (SMsubm02) or the entire m02 gene family (SMsubm02-16) grew like their wild-type parent in cultured cells. However, whereas SMsubm02 was as pathogenic as the wild-type virus, SMsubm02-16 was markedly less virulent. SMsubm02-16 produced less infectious virus in most organs compared to wild-type virus in BALB/c and C57BL/6J mice, but it replicated to wild-type levels in the organs of immunodeficient gamma(c)/Rag2 mice, lacking multiple cell types including natural killer cells, and in C57BL/6J mice depleted of natural killer cells. These results argue that one or more members of the m02 gene family antagonize natural killer cell-mediated immune surveillance. 相似文献
992.
Castilleja chambersii (Scrophulariaceae), a new rare species from the northern Coast Range of Oregon
Castilleja chambersii is described from several collections made in the Coast Range of southwestern Clatsop County, Oregon. The new species is
a member of subgenusCastilleja and is most closely related toC. parviflora andC. rupicola. This rare species is known from only three small and geographically restricted populations. Two new meiotic chromosome counts
ofn=12 are reported for the new species. 相似文献
993.
994.
995.
Reginald B. Cocroft 《Journal of Insect Behavior》2003,16(1):79-95
Many membracid treehoppers are attended by honeydew-harvesting ants. Ant mutualism often favors group living, which will in turn influence social interactios and communication. I investigated aspects of life history that underlie the social behavior of an aggregating, ant-attended treehopper. The number of adults, and their patterns of distribution, changes dramatically over the course of a season. Despite the relatively low vagility and high persistence in the same clump of host plants, individuals encounter a wide range of social environments. This aggregating species differs from solitary species in the clumped distribution of females, and possibly in the intensity of acoustic competition among males, but both aggregating and solitary species exhibit large temporal changes in density. A high degree of temporal and spatial variation in the social environment is probably characteristic of many insects and may be an important source of selection on insect communication. 相似文献
996.
Andrew T. Hurly 《Animal behaviour》2003,66(4):751-761
I developed two versions of the twin threshold model (TTM) to assess risk-sensitive foraging decisions by rufous hummingbirds. The model incorporates energy thresholds for both starvation and reproduction and assesses how three reward distributions with a common mean but different levels of variance interact with these critical thresholds to determine fitness. Fitness, a combination of survival and reproduction, is influenced by both the amount of variance in the distributions and the relative position of the common mean between the thresholds. The model predicts that risk-intermediate foraging is often the optimal policy, and that risk aversion is favoured as the common mean of the distributions approaches the starvation threshold, whereas risk preference is favoured as the common mean approaches the reproduction threshold. Tests with free-living hummingbirds supported these predictions. Hummingbirds were presented with three distributions of nectar rewards that had a common mean but Nil, Moderate or High levels of variance. Birds preferred intermediate levels of variance (Moderate) when presented with all three rewards simultaneously, and became more risk-averse as the mean of the distributions was decreased but more risk-prone as the mean was increased. Birds preferred Nil when it was paired with Moderate or with High, but preferred Moderate in the presence of Nil and High together. This reversal of preference is a violation of regularity, conventionally interpreted as irrational choice behaviour. I provide an alternative version of the TTM demonstrating that violations of regularity can occur when relative instead of absolute evaluation mechanisms are used. 相似文献
997.
Tissue culture techniques for the propagation and conservation of endemic or threatened plants can be used to complement the methods usually applied in ex situ conservation. Thus, Minuartia valentina (Caryophyllaceae), an endangered plant species endemic to the Valencia Community (Eastern Spain), was successfully regenerated through shoot proliferation from wild plants growing in their natural area. Nodal segments, 10~mm long, were cut from rametes of adult material, sterilised and established in vitro. Equally successful shoot multiplication was achieved on Murashige and Skoog (MS) medium with 80 mg l-1 phloroglucinol in combination with either 1 mg l-1 6-benzylaminopurine or 1 mg l-1 kinetin. Excised shoots were rooted in MS medium supplemented with an auxin (indole acetic acid, indole-3-butyric acid, or napththalene acetic acid). Shoots rooted well (96–100%) within three weeks in all auxin treatments. However, the use of napththalene acetic acid was discarded because this auxin delayed root differentiation, and induced adventitious root malformation. Rooted plantlets were transferred to pots and 85% of them acclimatized successfully four weeks after transfer to greenhouse conditions, where they exhibited normal morphology and growth. 相似文献
998.
999.
1000.
Published gene frequency data, checked for consistency of allele definitions across laboratories and for comparability of
geographically identical samples, were pooled into a data set containing frequencies at nine loci for each of 20 populations
that encompassed 10 macaque species. Genetic distances were calculated by the methods of Kidd and Cavalli-Sforza (1974). These
distances were used to construct phylogenetic trees and to evaluate the relationships between divergence times and effective
population sizes. Inter-and intraspecific genetic distances and the groupings defined by phenetic tree analyses support Fooden’s
(1976) classification of the genus Macacainto four species groups. A paleozoogeographical model of Asia including the known times of major sea-level changes allows
us to explain Macacainto four species groups. A paleozoogeographical model of Asia including the known times of major sea-level changes allows
us to explain qualitatively the inferred evolutionary relationships among macaque species. Many assumptions are required in
order to estimate the variables necessary in the quantitative prediction of genetic differences for a comparison between any
two populations. Examination of those assumptions demonstrates the need for more accurate genetic as well as paleozoogeographic
information.
An erratum to this article is available at . 相似文献