全文获取类型
收费全文 | 661518篇 |
免费 | 72782篇 |
国内免费 | 358篇 |
专业分类
734658篇 |
出版年
2016年 | 6897篇 |
2015年 | 9502篇 |
2014年 | 11274篇 |
2013年 | 16580篇 |
2012年 | 18204篇 |
2011年 | 18611篇 |
2010年 | 12720篇 |
2009年 | 11759篇 |
2008年 | 16723篇 |
2007年 | 17535篇 |
2006年 | 16576篇 |
2005年 | 16065篇 |
2004年 | 15936篇 |
2003年 | 15804篇 |
2002年 | 15331篇 |
2001年 | 25924篇 |
2000年 | 26021篇 |
1999年 | 21107篇 |
1998年 | 7862篇 |
1997年 | 8314篇 |
1996年 | 8060篇 |
1995年 | 7514篇 |
1994年 | 7427篇 |
1993年 | 7459篇 |
1992年 | 18712篇 |
1991年 | 18329篇 |
1990年 | 18213篇 |
1989年 | 17940篇 |
1988年 | 16840篇 |
1987年 | 16233篇 |
1986年 | 15138篇 |
1985年 | 15691篇 |
1984年 | 13106篇 |
1983年 | 11437篇 |
1982年 | 8952篇 |
1981年 | 8257篇 |
1980年 | 7682篇 |
1979年 | 12927篇 |
1978年 | 10116篇 |
1977年 | 9523篇 |
1976年 | 9208篇 |
1975年 | 9970篇 |
1974年 | 10910篇 |
1973年 | 10683篇 |
1972年 | 9944篇 |
1971年 | 8994篇 |
1970年 | 7855篇 |
1969年 | 7852篇 |
1968年 | 7242篇 |
1967年 | 6223篇 |
排序方式: 共有10000条查询结果,搜索用时 9 毫秒
991.
Severe aortic valve stenosis (AVS) and mitral valve regurgitation (MVR) often coexist. Although a fully percutaneous treatment for the two conditions, by means of transcatheter aortic valve implantation (TAVI) followed by MitraClip, can be appealing in selected high-risk candidates, critical and strategical reasoning should be applied. In a 3-year period we have developed a single-centre experience of 14 patients who were managed with a staged percutaneous approach to treat severe AVS and MVR. The average interval from TAVI to MitraClip repair was 101 ± 12 days. Success for TAVI was 100% and 92.9% (13/14) for MitraClip. At late follow-up, 3 patients developed MVR 3+. Estimated 1?year survival was 66.5%. Freedom from 1?year endpoint (death, stroke, major bleeding, myocardial infarction, and cardiac re-hospitalisation) was 57.9%.In our view, a fully transcatheter approach for mitro-aortic pathology is feasible and should be performed only as a staged procedure in those patients that remain symptomatic, in spite of successful TAVI. It should be emphasised that although the periprocedural success rate is satisfactory, follow-up mortality and re-hospitalisation rates remain high, even at mid-term follow-up. This most probably results from the advanced clinical picture at time of referral for treatment. 相似文献
992.
993.
994.
995.
W. H. Inman 《BMJ (Clinical research ed.)》1994,309(6954):608-609
996.
997.
The active moieties of the anti-biofilm natural compounds zosteric (ZA) and salicylic (SA) acids have been covalently immobilized on a low density polyethylene (LDPE) surface. The grafting procedure provided new non-toxic eco-friendly materials (LDPE-CA and LDPE-SA) with anti-biofilm properties superior to the conventional biocide-based approaches and with features suitable for applications in challenging fields where the use of antimicrobial agents is limited. Microbiological investigation proved that LDPE-CA and LDPE-SA: (1) reduced Escherichia coli biofilm biomass by up to 61% with a mechanism that did not affect bacterial viability; (2) significantly affected biofilm morphology, decreasing biofilm thickness, roughness, substratum coverage, cell and matrix polysaccharide bio-volumes by >80% and increasing the surface to bio-volume ratio; (3) made the biofilm more susceptible to ampicillin and ethanol. Since no molecules were leached from the surface, they remained constantly effective and below the lethal level; therefore, the risk of inducing resistance was minimized. 相似文献
998.
999.
A sucrase gene from Leuconostoc mesenteroides was cloned and expressed in Escherichia coli. The cloned enzyme did not show dextransucrase or sucrose phosphorylase activity. HPLC and GC-MS analyses of the sucrase products indicated the presence of fructose and glucose in equimolar amounts. IPTG induction did not increase sucrase activity in E. coli indicating that the cloned gene may be transcribed from its own promoter. To our knowledge, this is the first sucrase cloned from L. mesenteroides that has invertase activity. 相似文献
1000.
Hydrogen production by rumen holotrich protozoa: effects of oxygen and implications for metabolic control by in situ conditions 总被引:1,自引:0,他引:1
Experiments with washed suspensions of holotrich protozoa (Isotricha spp. and Dasytricha ruminantium) showed that both organisms have an efficient O2-scavenging capability (apparent Km values 2.3 and 0.3 microM, respectively). Reversible inhibition of H2 production increased almost linearly with increasing O2 up to 1.5 microM; higher levels of O2 gave irreversible inhibition. In situ determinations of H2, CH4, O2 and CO2 in ovine rumen liquor, using a membrane inlet mass spectrometer probe, indicated that O2 was present before feeding at 1-1.5 microM and decreased to undetectable levels (less than 0.25 microM) within 25 min after feeding. A transient increase in O2 concentration after feeding occurred only in defaunated animals and resulted in suppression of CH4 and CO2 production. The presence of washed holotrich protozoa decreases the O2 sensitivity of CH4 production by suspensions of a cultured methanogenic bacterium Methanosarcina barkeri. It is concluded that holotrich protozoa play a role in ruminal O2 utilization as well as in the production of fermentation end products (especially short-chain volatile fatty acids) utilized by the ruminant and H2 utilized by methanogenic bacteria. These hydrogenosome-containing protozoa thus both control patterns of fermentation by influencing O2 levels, and are themselves regulated by the low ambient O2 concentrations they experience in the rumen. 相似文献