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131.
Valkonen A Lahtinen M Virtanen E Kaikkonen S Kolehmainen E 《Biosensors & bioelectronics》2004,20(6):1233-1241
Simple bile acid amide synthesis of lithocholic and deoxycholic acids with 2-aminoethanol and 3-aminopropanol are reported. The structural properties of these amides were examined by NMR spectroscopic, ESI-TOF mass spectral, and X-ray crystallographic methods. The gelation properties of these amides in common organic solvents and in three different water solutions were also investigated using Tyndall effect, SEM, TEM, and optical microscopy. 2-Hydroxyethylamides were found to be effective gelators in chlorinated organic solvents and 3-hydroxypropylamides in aromatic solvents. Both derivatives thicken neutral and acidic water solutions. 相似文献
132.
Modulation of humoral immune response through probiotic intake 总被引:7,自引:0,他引:7
Thirty healthy volunteers were randomised into three different treatment groups and consumed Lactobacillus GG, Lactococcus lactis or placebo (ethyl cellulose) for 7 days. On days 1, 3 and 5, an attenuated Salmonella typhi Ty21a oral vaccine was given to all subjects to mimic an enteropathogenic infection. All subjects responded well to the vaccine, but no significant differences were observed in numbers of IgA-, IgG- and IgM-secreting cells among the different groups. There was a trend towards a greater increase in specific IgA among the subjects receiving the vaccine in combination with Lactobacillus GG. Those receiving L. lactis with their vaccine evinced significantly higher CR3 receptor expression on neutrophils than those receiving either the placebo or Lactobacillus GG. These results indicate that probiotics may influence differently the immune response to oral S. typhi vaccine and that the immunomodulatory effect of probiotics is strain-dependent. 相似文献
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134.
Differential expression of the Arabidopsis cytochrome c genes Cytc-1 and Cytc-2. Evidence for the involvement of TCP-domain protein-binding elements in anther- and meristem-specific expression of the Cytc-1 gene
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The promoters of the Arabidopsis (Arabidopsis thaliana) cytochrome c genes, Cytc-1 and Cytc-2, were analyzed using plants transformed with fusions to the beta-glucuronidase coding sequence. Histochemical staining of plants indicated that the Cytc-1 promoter directs preferential expression in root and shoot meristems and in anthers. In turn, plants transformed with the Cytc-2 promoter fusions showed preferential expression in vascular tissues of cotyledons, leaves, roots, and hypocotyls, and also in anthers. Quantitative measurements in extracts prepared from different organs suggested that expression of Cytc-1 is higher in flowers, while that of Cytc-2 is higher in leaves. The analysis of a set of deletions and site-directed mutants of the Cytc-1 promoter indicated that a segment located between -147 and -156 from the translation start site is required for expression and that site II elements (TGGGCC/T) located in this region, coupled with a downstream internal telomeric repeat (AAACCCTAA), are responsible for the expression pattern of this gene. Proteins present in cauliflower nuclear extracts, as well as a recombinant protein from the TCP-domain family, were able to specifically bind to the region required for expression. We propose that expression of the Cytc-1 gene is linked to cell proliferation through the elements described above. The fact that closely located site II motifs are present in similar locations in several genes encoding proteins involved in cytochrome c-dependent respiration suggests that these elements may be the target of factors that coordinate the expression of nuclear genes encoding components of this part of the mitochondrial respiratory chain. 相似文献
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The tritrophic interactions between plants, herbivores and avian predators are complex and prone to trophic cascades. We conducted a meta-analysis of original articles that have studied birds as predators of invertebrate herbivores, to compare top-down trophic cascades with different plant responses from different environments and climatic areas. Our search found 29 suitable articles, with a total of 81 separate experimental study set-ups. The meta-analysis revealed that plants benefited from the presence of birds. A significant reduction was observed in the level of leaf damage and plant mortality. The presence of birds also positively affected the amount of plant biomass, whereas effects on plant growth were negligible. There were no differences in the effects between agricultural and natural environments. Similarly, plants performed better in all climatic areas (tropical, temperate and boreal) when birds were present. Moreover, both mature plants and saplings gained benefits from the presence of birds. Our results show that birds cause top-down trophic cascades and thus they play an integral role in ecosystems. 相似文献
138.
Gavin M. Lewis Monica Macal Charles R. Hesser Elina I. Zu?iga 《Journal of virology》2015,89(6):3343-3355
139.
Population and reproductive biology of four Potamolithus species, two epigean (P. ribeirensis and Potamolithus sp. 1) and two subterranean (P. troglobius and Potamolithus sp. 2), from the Upper Ribeira Valley, southeast Brazil, were studied, from June 1996 to June 1997, using 1 m2 quadrats and counting all individuals found in these quadrats. P. ribeirensis showed the highest variation in population densities (from 0 – after floods – to 467 ind.m?2), with average ranges from 16.0 to 284.8 ind.m?2. The densities of the two troglobitic species (average ranges for Potamolithus sp. 2 and P. troglobius, from 17.6 to 58.4 and from 0 to 61.5 ind.m?2, respectively), higher than those of Potamolithus sp. 1 (average from 0 to 9.3 ind.m?2), were high for cave organisms. P. ribeirensis and the two troglobitic species showed higher densities in warmer, wetter months, while Potamolithus sp. 1 showed higher densities in cooler, less rainy months. The troglobitic species were non-seasonal, since high percentages of mature females were observed throughout the year. In the epigean species, the reproductive periods showed no detectable pattern: in 1996 P. ribeirensis had a peak in winter and in the next year (1997) in spring and summer. Potamolithus sp. 1 had reproductive peaks in fall and winter. 相似文献
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