全文获取类型
收费全文 | 191篇 |
免费 | 23篇 |
出版年
2023年 | 3篇 |
2022年 | 3篇 |
2021年 | 7篇 |
2020年 | 9篇 |
2019年 | 5篇 |
2018年 | 9篇 |
2017年 | 9篇 |
2016年 | 7篇 |
2015年 | 14篇 |
2014年 | 12篇 |
2013年 | 16篇 |
2012年 | 15篇 |
2011年 | 5篇 |
2010年 | 5篇 |
2009年 | 7篇 |
2008年 | 7篇 |
2007年 | 5篇 |
2006年 | 6篇 |
2005年 | 6篇 |
2004年 | 11篇 |
2003年 | 9篇 |
2002年 | 5篇 |
2001年 | 3篇 |
2000年 | 3篇 |
1999年 | 4篇 |
1998年 | 1篇 |
1997年 | 1篇 |
1996年 | 3篇 |
1994年 | 1篇 |
1993年 | 1篇 |
1992年 | 3篇 |
1990年 | 1篇 |
1989年 | 3篇 |
1988年 | 2篇 |
1987年 | 2篇 |
1986年 | 1篇 |
1985年 | 2篇 |
1984年 | 1篇 |
1982年 | 2篇 |
1981年 | 1篇 |
1980年 | 1篇 |
1979年 | 1篇 |
1978年 | 1篇 |
1976年 | 1篇 |
排序方式: 共有214条查询结果,搜索用时 31 毫秒
91.
Background
Mycobacteria have developed a number of pathways that provide partial protection against both reactive oxygen species (ROS) and reactive nitrogen species (RNS). We recently identified a locus in Mycobacterium marinum, mel2, that plays a role during infection of macrophages. The molecular mechanism of mel2 action is not well understood. 相似文献92.
Trine M. Reine Tram Thu Vuong Trond G. Jenssen Svein O. Kolset 《Biochimica et Biophysica Acta (BBA)/General Subjects》2014
Background
Endothelial cells have important functions in e.g. regulating blood pressure, coagulation and host defense reactions. Serglycin is highly expressed by endothelial cells, but there is limited data on the roles of this proteoglycan in immune reactions.Methods
Cultured primary human endothelial cells were exposed to proinflammatory agents lipopolysaccharide (LPS) and interleukin 1β (IL-1β). The response in serglycin synthesis, secretion and intracellular localization and effect on the proteoglycan binding chemokines CXCL-1 and CXCL-8 were determined by qRT-PCR, Western blotting, immunocytochemistry, ELISA and serglycin knockdown experiments.Results
Both LPS and IL-1β increased the synthesis and secretion of serglycin, while only IL-1β increased serglycin mRNA expression. Stimulation increased the number of serglycin containing vesicles, with a greater portion of large vesicles after LPS treatment. Also, increased intracellular and secreted levels of CXCL-1 and CXCL-8 were observed. The increase in CXCL-8 secretion was unchanged in serglycin knockdown cells. However, the increase in CXCL-1 secretion from IL-1β stimulation was reduced 27% in serglycin knockdown cells; while the LPS-induced secretion was not affected. In serglycin expressing cells CXCL-1 positive vesicles were evenly distributed throughout the cytoplasm, while confided to the Golgi region in serglycin knockdown cells. This was the case only for IL-1β stimulated cells. LPS-induced CXCL-1 distribution was unaffected by serglycin expression.Conclusions
These results suggest that different signaling pathways are involved in regulating secretion of serglycin and partner molecules in activated endothelial cells.General significance
This knowledge increases our understanding of the roles of serglycin in immune reactions. This article is part of a Special Issue entitled: Matrix-mediated cell behaviour and properties. 相似文献93.
Laura Martinez-Rubio ?ystein Evensen Aleksei Krasnov Sven Martin J?rgensen Simon Wadsworth Kari Ruohonen Jose LG Vecino Douglas R Tocher 《BMC genomics》2014,15(1)
Background
Cardiomyopathy syndrome (CMS) is a severe cardiac disease of Atlantic salmon (Salmo salar) recently associated with a double-stranded RNA virus, Piscine Myocarditis Virus (PMCV). The disease has been diagnosed in 75-85 farms in Norway each year over the last decade resulting in annual economic losses estimated at up to €9 million. Recently, we demonstrated that functional feeds led to a milder inflammatory response and reduced severity of heart lesions in salmon experimentally infected with Atlantic salmon reovirus, the causal agent of heart and skeletal muscle inflammation (HSMI). In the present study we employed a similar strategy to investigate the effects of functional feeds, with reduced lipid content and increased eicosapentaenoic acid levels, in controlling CMS in salmon after experimental infection with PMCV.Results
Hepatic steatosis associated with CMS was significantly reduced over the time course of the infection in fish fed the functional feeds. Significant differences in immune and inflammatory responses and pathology in heart tissue were found in fish fed the different dietary treatments over the course of the infection. Specifically, fish fed the functional feeds showed a milder and delayed inflammatory response and, consequently, less severity of heart lesions at earlier and later stages after infection with PMCV. Decreasing levels of phosphatidylinositol in cell membranes combined with the increased expression of genes related with T-cell signalling pathways revealed new interactions between dietary lipid composition and the immune response in fish during viral infection. Dietary histidine supplementation did not significantly affect immune responses or levels of heart lesions.Conclusions
Combined with the previous findings on HSMI, the results of the present study highlight the potential role of clinical nutrition in controlling inflammatory diseases in Atlantic salmon. In particular, dietary lipid content and fatty acid composition may have important immune-modulatory effects in Atlantic salmon that could be potentially beneficial in fish balancing the immune and tissue responses to viral infections.Electronic supplementary material
The online version of this article (doi:10.1186/1471-2164-15-462) contains supplementary material, which is available to authorized users. 相似文献94.
95.
Novel quantitative trait loci for broad-based resistance to soybean cyst nematode (Heterodera glycines Ichinohe) in soybean PI 567516C 总被引:1,自引:0,他引:1
Tri D. Vuong David A. Sleper James G. Shannon Henry T. Nguyen 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2010,121(7):1253-1266
Soybean cyst nematode (SCN, Heterodera glycines Ichinohe) is the most destructive pest of soybean worldwide. Host plant resistance is an effective approach to control this
pest. Plant introduction PI 567516C has been reported to be highly resistant to multiple-HG types of SCN. The objectives of
this study were to identify and map novel quantitative trait loci (QTL) for SCN resistance to six HG types (also known as
races 1, 2, 3, 5, 14, and LY1). Mapping was conducted using 250 F2:3 progeny derived from a Magellan (susceptible) × PI 567516C (resistant) cross. F6:7 recombinant inbred lines (RILs) developed from the F2:3 progeny were employed to confirm the putative QTL identified. A total of 927 polymorphic simple sequence repeats (SSR) and
single nucleotide polymorphism (SNP) markers were genotyped. Following the genetic linkage analysis, permutation tests and
composite interval mapping were performed to identify and map QTL. Four QTL were associated with resistance to either multiple-
or single-SCN HG types. Two QTL for resistance to multiple-SCN HG types were mapped to Chromosomes 10 and 18 and have not
been reported in other SCN resistance sources. New QTL were confirmed by analysis of 250 F6:7 RILs from the same population. SSR and SNP markers closely associated with these QTL can be useful for the development of
near-isogenic lines for fine-mapping and positional cloning of candidate genes for SCN resistance. 相似文献
96.
97.
Wu X Vuong TD Leroy JA Grover Shannon J Sleper DA Nguyen HT 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2011,122(6):1179-1187
Soybean BAC-based physical maps provide a useful platform for gene and QTL map-based cloning, EST mapping, marker development,
genome sequencing, and comparative genomic research. Soybean physical maps for “Forrest” and “Williams 82” representing the
southern and northern US soybean germplasm base, respectively, have been constructed with different fingerprinting methods.
These physical maps are complementary for coverage of gaps on the 20 soybean linkage groups. More than 5,000 genetic markers
have been anchored onto the Williams 82 physical map, but only a limited number of markers have been anchored to the Forrest
physical map. A mapping population of Forrest × Williams 82 made up of 1,025 F8 recombinant inbred lines (RILs) was used to construct a reference genetic map. A framework map with almost 1,000 genetic
markers was constructed using a core set of these RILs. The core set of the population was evaluated with the theoretical
population using equality, symmetry and representativeness tests. A high-resolution genetic map will allow integration and
utilization of the physical maps to target QTL regions of interest, and to place a larger number of markers into a map in
a more efficient way using a core set of RILs. 相似文献
98.
Sialyl Lewis(x) (SLe(x), Siaα2-3Galβ1-4(Fucα1-3)GlcNAcβOR) is an important sialic acid-containing carbohydrate epitope involved in many biological processes such as inflammation and cancer metastasis. In the biosynthetic process of SLe(x), α2-3-sialyltransferase-catalyzed sialylation generally proceeds prior to α1-3-fucosyltransferase-catalyzed fucosylation. For the chemoenzymatic synthesis of SLe(x) containing different sialic acid forms, however, it would be more efficient if diverse sialic acid forms are transferred in the last step to the fucosylated substrate Lewis(x) (Le(x)). An α2-3-sialyltransferase obtained from myxoma virus-infected European rabbit kidney RK13 cells (viral α2-3-sialyltransferase (vST3Gal-I)) was reported to be able to tolerate fucosylated substrate Le(x). Nevertheless, the substrate specificity of the enzyme was only determined using partially purified protein from extracts of cells infected with myxoma virus. Herein we demonstrate that a previously reported multifunctional bacterial enzyme Pasteurella multocida sialyltransferase 1 (PmST1) can also use Le(x) as an acceptor substrate, although at a much lower efficiency compared to nonfucosylated acceptor. In addition, N-terminal 30-amino-acid truncated vST3Gal-I has been successfully cloned and expressed in Escherichia coli Origami? B(DE3) cells as a fusion protein with an N-terminal maltose binding protein (MBP) and a C-terminal His(6)-tag (MBP-Δ30vST3Gal-I-His(6)). The viral protein has been purified to homogeneity and characterized biochemically. The enzyme is active in a broad pH range varying from 5.0 to 9.0. It does not require a divalent metal for its α2-3-sialyltransferase activity. It has been used in one-pot multienzyme sialylation of Le(x) for the synthesis of SLe(x) containing different sialic acid forms with good yields. 相似文献
99.
Pham Hong Ngoc Thuy Vuong Quan Van Bowyer Michael C. Scarlett Christopher J. 《Molecular biology reports》2020,47(2):897-905
Molecular Biology Reports - Pancreatic cancer (PC) is one of the leading causes of cancer death in Western societies. The absence of specific symptoms, late diagnosis and the resistance towards... 相似文献
100.
Jian-Yong Zeng Thi-Minh-Dien Vuong Jia-Xing Guo Jiang-Hong Shi Zhong-Bin Shi Guo-Cai Zhang Jie Zhang 《Archives of insect biochemistry and physiology》2020,104(3):e21691
In the present study, diel pattern in gut microbial communities in insects were evaluated. Lymantria dispar asiatica fourth instar larvae (72 ± 2 hr after molting) at noon (LdD) and midnight (LdN) were used for a comparative analysis of the gut microbial community. Ten bacterial operational taxonomic units (OTUs) were shared between LdD and LdN samples. One bacterial OTU was specific to LdD. The dominant gut microbes were OTU72 in LdD and OTU75 in LdN. A linear discriminant analysis effect size cladogram suggested that ten bacterial OTUs maintain significant differences in relative abundances between LdD and LdN. These results agreed with the discrete ellipses between LdD and LdN in principal coordinates analysis plots. Additionally, using phylogenetic investigation of communities by reconstruction of unobserved states, the gut microbial community was assigned to 23 functional terms, among which 22 exhibited significant differences between LdD and LdN. To conclude, the present study documented a diel pattern in the gut microbial community of L. dispar asiatica larvae. 相似文献