全文获取类型
收费全文 | 1491篇 |
免费 | 101篇 |
国内免费 | 2篇 |
出版年
2023年 | 18篇 |
2022年 | 40篇 |
2021年 | 55篇 |
2020年 | 26篇 |
2019年 | 35篇 |
2018年 | 40篇 |
2017年 | 34篇 |
2016年 | 53篇 |
2015年 | 93篇 |
2014年 | 74篇 |
2013年 | 120篇 |
2012年 | 117篇 |
2011年 | 149篇 |
2010年 | 55篇 |
2009年 | 73篇 |
2008年 | 80篇 |
2007年 | 72篇 |
2006年 | 66篇 |
2005年 | 45篇 |
2004年 | 62篇 |
2003年 | 38篇 |
2002年 | 41篇 |
2001年 | 24篇 |
2000年 | 30篇 |
1999年 | 23篇 |
1998年 | 12篇 |
1997年 | 2篇 |
1996年 | 5篇 |
1995年 | 3篇 |
1994年 | 3篇 |
1993年 | 8篇 |
1992年 | 10篇 |
1991年 | 11篇 |
1990年 | 12篇 |
1989年 | 9篇 |
1988年 | 10篇 |
1987年 | 8篇 |
1986年 | 1篇 |
1985年 | 4篇 |
1984年 | 7篇 |
1983年 | 8篇 |
1982年 | 4篇 |
1981年 | 4篇 |
1979年 | 1篇 |
1976年 | 3篇 |
1974年 | 1篇 |
1972年 | 2篇 |
1971年 | 3篇 |
排序方式: 共有1594条查询结果,搜索用时 109 毫秒
111.
Christopher S. Bland Eric T. Wang Anthony Vu Marjorie P. David John C. Castle Jason M. Johnson Christopher B. Burge Thomas A. Cooper 《Nucleic acids research》2010,38(21):7651-7664
Recent genome-wide analyses have elucidated the extent of alternative splicing (AS) in mammals, often focusing on comparisons of splice isoforms between differentiated tissues. However, regulated splicing changes are likely to be important in biological transitions such as cellular differentiation, or response to environmental stimuli. To assess the extent and significance of AS in myogenesis, we used splicing-sensitive microarray analysis of differentiating C2C12 myoblasts. We identified 95 AS events that undergo robust splicing transitions during C2C12 differentiation. More than half of the splicing transitions are conserved during differentiation of avian myoblasts, suggesting the products and timing of transitions are functionally significant. The majority of splicing transitions during C2C12 differentiation fall into four temporal patterns and were dependent on the myogenic program, suggesting that they are integral components of myogenic differentiation. Computational analyses revealed enrichment of many sequence motifs within the upstream and downstream intronic regions near the alternatively spliced regions corresponding to binding sites of splicing regulators. Western analyses demonstrated that several splicing regulators undergo dynamic changes in nuclear abundance during differentiation. These findings show that within a developmental context, AS is a highly regulated and conserved process, suggesting a major role for AS regulation in myogenic differentiation. 相似文献
112.
Selvaraj T Kim SK Kim YH Jeong YS Kim YJ Phuong ND Jung KH Kim J Yun HD Kim H 《Journal of microbiology (Seoul, Korea)》2010,48(6):856-861
A non-cellulosomal xylanase from Clostridium thermocellum, XynX, consists of a family-22 carbohydratebinding module (CBM22), a family-10 glycoside hydrolase (GH10) catalytic module, two family-9 carbohydrate-binding modules (CBM9-I and CBM9-II), and an S-layer homology (SLH) module. E. coli BL21(DE3) (pKM29), a transformant carrying xynX', produced several truncated forms of the enzyme. Among them, three major active species were purified by SDS-PAGE, activity staining, gel-slicing, and diffusion from the gel. The truncated xylanases were different from each other only in their C-terminal regions. In addition to the CBM22 and GH10 catalytic modules, XynX(1) had the CBM9-I and most of the CBM9-II, XynX(2) had the CBM9-I and about 40% of the CBM9-II, and XynX(3) had about 75% of the CBM9-I. The truncated xylanases showed higher binding capacities toward Avicel than those toward insoluble xylan. XynX(1) showed a higher affinity toward Avicel (70.5%) than XynX(2) (46.0%) and XynX(3) (42.1%); however, there were no significant differences in the affinities toward insoluble xylan. It is suggested that the CBM9 repeat, especially CBM9-II, of XynX plays a role in xylan degradation in nature by strengthening cellulose binding rather than by enhancing xylan binding. 相似文献
113.
114.
115.
Heterosubtypic immunity to influenza A virus infection requires a properly diversified antibody repertoire
下载免费PDF全文
![点击此处可从《Journal of virology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Nguyen HH Zemlin M Ivanov II Andrasi J Zemlin C Vu HL Schelonka R Schroeder HW Mestecky J 《Journal of virology》2007,81(17):9331-9338
Heterosubtypic immunity (HSI) is defined as cross-protection to infection with an influenza A virus serotype other than the one used for primary infection. Although HSI has been thought to be mediated by serotype cross-reactive cytotoxic T lymphocytes (CTL) that recognize conserved epitopes of structural proteins, recent studies suggest that antibodies (Abs) may make a significant contribution. In this study, we provide further evidence for the role of Abs in HSI using transgenic mice lacking terminal deoxyribonucleotidyltransferase (TdT), which adds N nucleotides to V-D and D-J junctions of the complementary determining region 3 (CDR3) (TdT(-/-)) and mice with altered Ab repertoires due to replacement of the complete locus of heavy chain diversity segments (D(H)) with an altered D(H) segment (namely, Delta D-iD). Both types of mice failed to generate complete HSI, although they were able to mount protective immunity to a homologous challenge. Lower levels of virus-specific antibodies along with more severely impaired HSI were observed in TdT(-/-) mice compared to those in Delta D-iD mice, while CTL activity remained unchanged in both types of mice. These findings indicate that a properly diversified antibody repertoire is required for HSI and that N addition by TdT is a more effective mechanism in the induction of a properly diversified antibody repertoire and, therefore, complete HSI. The results suggest that the diversity of the antibody repertoire as determined by the composition of the D region of HCDR3 and by N addition are among the mechanisms selected for in evolution to create a favorable environment to resolve infections with mutated viruses. 相似文献
116.
We describe the construction of six novel plasmid-based IPTG-inducible expression vectors for Bacillus subtilis and related species. While one vector allows intracellular production of recombinant proteins, the second provides a strong
secretion signal. The third vector allows addition of the c-Myc epitope tag, and the remaining three vectors provide the purification tags His and Strep. The versatility of all six
vectors was demonstrated by the insertion of several reporter genes and by their regulated overexpression. Recombinant proteins
with a His- or Strep-tag could be purified to near homogeneity in a single step. 相似文献
117.
Gore VK Ma VV Tamir R Gavva NR Treanor JJ Norman MH 《Bioorganic & medicinal chemistry letters》2007,17(21):5825-5830
A novel series of 4,5-biarylimidazoles as TRPV1 antagonists were designed based on the previously reported 4,6-disubstituted benzimidazole series. The analogs were evaluated for their ability to block capsaicin- or acid-induced calcium influx in TRPV1-expressing CHO cells. These studies led to the identification of a highly potent and orally bioavailable TRPV1 antagonist, imidazole 33. 相似文献
118.
Vu AT Campbell AN Harris HA Unwalla RJ Manas ES Mewshaw RE 《Bioorganic & medicinal chemistry letters》2007,17(14):4053-4056
A new class of estrogen receptor beta (ERbeta) ligands based on the 6H-chromeno[4,3-b]quinoline scaffold has been prepared. Several C7-substituted analogues displayed high affinity and modest selectivity for ERbeta. 相似文献
119.
120.
Binh Tran Quang Linh Duong Tuan Chung Le Thi Kim Phuong Pham Tran Nga Bui Thi Thuy Ngoc Nguyen Anh Thuyen Tran Quang Tung Do Dinh Nhung Bui Thi 《Biochemical genetics》2022,60(2):707-719
Biochemical Genetics - The study aimed to evaluate the contribution of the FTO A/T polymorphism (rs9939609) to the prediction of the future type 2 diabetes (T2D). A population-based prospective... 相似文献