全文获取类型
收费全文 | 215篇 |
免费 | 13篇 |
出版年
2023年 | 1篇 |
2022年 | 1篇 |
2021年 | 6篇 |
2020年 | 3篇 |
2018年 | 1篇 |
2017年 | 2篇 |
2016年 | 8篇 |
2015年 | 11篇 |
2014年 | 13篇 |
2013年 | 14篇 |
2012年 | 23篇 |
2011年 | 16篇 |
2010年 | 15篇 |
2009年 | 9篇 |
2008年 | 14篇 |
2007年 | 10篇 |
2006年 | 16篇 |
2005年 | 10篇 |
2004年 | 4篇 |
2003年 | 12篇 |
2002年 | 8篇 |
2001年 | 2篇 |
2000年 | 5篇 |
1999年 | 3篇 |
1998年 | 1篇 |
1997年 | 5篇 |
1996年 | 1篇 |
1995年 | 2篇 |
1994年 | 1篇 |
1991年 | 1篇 |
1990年 | 1篇 |
1989年 | 1篇 |
1988年 | 3篇 |
1985年 | 2篇 |
1984年 | 1篇 |
1983年 | 1篇 |
1957年 | 1篇 |
排序方式: 共有228条查询结果,搜索用时 62 毫秒
221.
Fire ephemerals are few in the boreal forest despite a long history of recurrent fires, which suggests such a life-history pose problems here. We analysed the fate of recruiting populations of two rare and fire-dependent annual Geranium species at burnt forest sites in South-eastern Sweden, to extract vital information on their life-history. Seedlings emerged from the soil seed bank only in the year of fire but spread over several weeks. At sites that burnt early in the season, some seedlings exhibited a summer-annual life-cycle, but those were less successful than plants at the same sites that delayed reproduction until the following year (winter-annuals). Herbivory was frequent in the fire year and until the following spring, but later almost absent, and thus hit seed production in summer-annuals badly. Winter mortality was highly variable for rosette-stage winter-annuals, with some populations nearly obliterated. Reproductive success varied greatly between populations mainly due to pre-reproductive mortality, with a return of 0.2–395 (average 79) seeds per seedling. The vast majority of seeds (92–100 %) were produced by the primary generation, emerging from the seed bank. Out of this first seed crop, 0.2–2.5 % germinated within the study period, resulting in secondary generations. Plants in these later generations were small and produced few seeds, showing that the opportunity for high reproductive success is essentially restricted to one year only. This makes populations highly vulnerable to local near-complete reproductive failure due to winter mortality and herbivory and may be the ultimate reason why strict fire ephemerals are so few in northern forests. 相似文献
222.
223.
Karsten Pedersen Johanna Arlinger Susanne Ekendahl Lotta Hallbeck 《FEMS microbiology ecology》1996,19(4):249-262
Abstract: A total of 155 16S rRNA genes that were cloned from unattached and attached bacteria in nine boreholes down to 626 m below ground were partially sequenced. Attached bacteria were examined with scanning electron microscopy (SEM). The distribution of the 16S rRNA genes found was related to the different types of groundwaters studied. Several of the sequences obtained could be identified on genus level as one of the genera Acinetobacter, Bacillus, Desulfovibrio or Thiomicrospira . The 16S rRNA genes from 20 selected isolates were closely related to the sulphate reducers Desulfomicrobium baculatum or Desulfovibrio sp., the iron reducer Shewanella putrefaciens , or distantly related to the Gram-positive genus Eubacterium . Viable counts confirmed the presence of sulphate-reducing bacteria. 相似文献
224.
225.
Jan Herzog Alexander Mook Lotta Guhl Miriam Bäumler Matthias H. Beck Dirk Weuster-Botz Frank R. Bengelsdorf An-Ping Zeng 《Engineering in Life Science》2023,23(1):e2100169
Acetobacterium woodii is known to produce mainly acetate from CO2 and H2, but the production of higher value chemicals is desired for the bioeconomy. Using chain-elongating bacteria, synthetic co-cultures have the potential to produce longer-chained products such as caproic acid. In this study, we present first results for a successful autotrophic co-cultivation of A. woodii mutants and a Clostridium drakei wild-type strain in a stirred-tank bioreactor for the production of caproic acid from CO2 and H2 via the intermediate lactic acid. For autotrophic lactate production, a recombinant A. woodii strain with a deleted Lct-dehydrogenase complex, which is encoded by the lctBCD genes, and an inserted D-lactate dehydrogenase (LdhD) originating from Leuconostoc mesenteroides, was used. Hydrogen for the process was supplied using an All-in-One electrode for in situ water electrolysis. Lactate concentrations as high as 0.5 g L–1 were achieved with the AiO-electrode, whereas 8.1 g L–1 lactate were produced with direct H2 sparging in a stirred-tank bioreactor. Hydrogen limitation was identified in the AiO process. However, with cathode surface area enlargement or numbering-up of the electrode and on-demand hydrogen generation, this process has great potential for a true carbon-negative production of value chemicals from CO2. 相似文献
226.
The changes in agricultural practices during the last century have led to a drastic decrease in the number of traditionally managed hay meadows. Also, traditional management practices are often applied more cursorily in the remaining meadows. In combination with an increase in aerial anthropogenic nitrogen deposition, this has led to a loss of biodiversity. To investigate whether the current management is sufficient for maintaining viable populations of a typical meadow plant, Succisa pratensis, we experimentally reinforced the raking and mowing parts of the traditional management over four years in a two-by-two factorial experiment in three traditionally managed wooded hay meadows on the Baltic island of Gotland, Sweden. We found decreased litter and hay production in two of the three studied meadows as a result of our treatments. Plant sizes and asymptotic population growth rates (??) of S. pratensis increased, particularly in plots receiving the combined raking and mowing treatment. Stochastic long-term population growth rates (?? s ) increased under the reinforced management: projected population sizes 50?years into the future showed a three-fold increase in raked plots and a 17-fold increase in plots that were both raked and mown. Because we found positive responses even in these seemingly well-managed meadows we conclude that it is essential that management is carried out more thoroughly to ensure viable population sizes. Our conclusion applies to most semi-natural grasslands receiving anthropogenic nitrogen, or where traditional management practices are less rigorously applied. We also suggest using biomass estimation instead of vegetation height as a measure of management strength. 相似文献
227.
Lotta J. Happonen Esko Oksanen Lassi Liljeroos Adrian Goldman Tommi Kajander Sarah J. Butcher 《Journal of virology》2013,87(15):8388-8398
Biochemical reactions powered by ATP hydrolysis are fundamental for the movement of molecules and cellular structures. One such reaction is the encapsidation of the double-stranded DNA (dsDNA) genome of an icosahedrally symmetric virus into a preformed procapsid with the help of a genome-translocating NTPase. Such NTPases have been characterized in detail from both RNA and tailed DNA viruses. We present four crystal structures and the biochemical activity of a thermophilic NTPase, B204, from the nontailed, membrane-containing, hyperthermoacidophilic archaeal dsDNA virus Sulfolobus turreted icosahedral virus 2. These are the first structures of a genome-packaging NTPase from a nontailed, dsDNA virus with an archaeal host. The four structures highlight the catalytic cycle of B204, pinpointing the molecular movement between substrate-bound (open) and empty (closed) active sites. The protein is shown to bind both single-stranded and double-stranded nucleic acids and to have an optimum activity at 80°C and pH 4.5. The overall fold of B204 places it in the FtsK-HerA superfamily of P-loop ATPases, whose cellular and viral members have been suggested to share a DNA-translocating mechanism. 相似文献
228.
Nematophagous fungi in the rhizosphere of agricultural crops 总被引:1,自引:0,他引:1