全文获取类型
收费全文 | 10083篇 |
免费 | 728篇 |
国内免费 | 6篇 |
专业分类
10817篇 |
出版年
2022年 | 60篇 |
2021年 | 115篇 |
2020年 | 73篇 |
2019年 | 86篇 |
2018年 | 119篇 |
2017年 | 95篇 |
2016年 | 170篇 |
2015年 | 341篇 |
2014年 | 377篇 |
2013年 | 537篇 |
2012年 | 640篇 |
2011年 | 632篇 |
2010年 | 462篇 |
2009年 | 381篇 |
2008年 | 570篇 |
2007年 | 550篇 |
2006年 | 515篇 |
2005年 | 524篇 |
2004年 | 518篇 |
2003年 | 515篇 |
2002年 | 451篇 |
2001年 | 112篇 |
2000年 | 103篇 |
1999年 | 131篇 |
1998年 | 167篇 |
1997年 | 98篇 |
1996年 | 124篇 |
1995年 | 110篇 |
1994年 | 94篇 |
1993年 | 111篇 |
1992年 | 106篇 |
1991年 | 86篇 |
1990年 | 83篇 |
1989年 | 76篇 |
1988年 | 92篇 |
1987年 | 76篇 |
1986年 | 59篇 |
1985年 | 83篇 |
1984年 | 112篇 |
1983年 | 94篇 |
1982年 | 108篇 |
1981年 | 94篇 |
1980年 | 94篇 |
1979年 | 73篇 |
1978年 | 82篇 |
1977年 | 84篇 |
1976年 | 71篇 |
1975年 | 64篇 |
1974年 | 74篇 |
1973年 | 65篇 |
排序方式: 共有10000条查询结果,搜索用时 0 毫秒
101.
102.
103.
Philip N. Sawyer Joel Levine Roger Mazlen Ignatius Valmont 《The Journal of general physiology》1961,45(2):181-196
Experiments giving evidence of active Na and Cl ion fluxes across large canine blood vessel walls (aorta, vena cava) in vitro have been presented. The information has been obtained using ion tracer techniques after Ussing and with diffusion cells of the Hogben type. The available data suggest that the membranes are satisfactorily oxygenated by the bathing solutions saturated with oxygen at atmospheric pressure. Evidence is offered which indicates that active ion transport does occur across the aorta and vena cava in in vitro experiments. Under the conditions of the experiment net Na and Cl flux takes place from intima to adventitia across the aorta, and from adventitia to intima across the vena cava at low measured potential differences. The possible relationships of derangement of active ion transport mechanisms, produced by electric currents and tissue injury potential differences, to intravascular thrombosis are alluded to. It would appear that sodium and chloride fluxes across large blood vessel walls in vitro occur at least in part as the result of metabolic processes and cannot be explained simply on the basis of diffusion across a semipermeable membrane. 相似文献
104.
It is important to make a diagnosis of prostatic carcinoma as early as possible, because early treatment gives the best results whether radical prostatectomy is done or endocrine therapy used. Open perineal biopsy is the most accurate method of making a diagnosis. Perineal needle biopsy or the newer approach of transrectal needle biopsy is probably about 75 per cent accurate in making a diagnosis.Ten-year survival with conservative therapy, as determined in a review of a series of cases, was 50 per cent—about the same as that following radical prostatectomy; but the patients with prostatectomy are clinically free of malignant disease whereas the former are not. Radical prostatectomy is indicated in a few selected cases.The results from endocrine therapy begun immediately after diagnosis are significantly better than those from delayed treatment. Orchiectomy and estrogens promise a little longer survival than estrogens alone. 相似文献
105.
Summary An investigation was made of 5 species of blue-green algae reported to contain gas-vacuoles. All organisms were grown and harvested under standard conditions. Gas-vacuoles were characterised as reddish structures which are destroyed by applying pressure. Using a simple direct preparation technique gascylinders were observed with the transmission electron microscope in gas-vacuolate cells. Gas-vacuoles were present in the strains of Anabaena flos-aquae, Gloeotrichia echinulata and Oscillatoria agardhii studied and absent from Microcystis aeruginosa and Nostoc linckia. The reddish, refractile central area of N. linckia and M. aeruginosa cells was tentatively identified as nucleoplasm. Gas-vacuoles are collections of gas-cylinders 70 m wide, which in A. flos-aquae and G. echinulata are clearly bounded by photosynthetic lamellae and associated with -granules. The presence of bounding photosynthetic lamellae in these species is suggested as a causal factor of the unusual optical properties of their gas-vacuoles. The range of lengths of gas-cylinders in G. echinulata and O. agardhii is from 100 m to 500 m and in A. flos-aquae it is from 100 m to 1300 m. The percentage of cell volume occupied by gas-vacuoles was estimated by direct measurement. In A. flos-aquae and G. echinulata it was 22%. In O. agardhii gas-cylinders were not clearly associated with photosynthetic lamellae and -granules and occupied 39% of cell volume. Gascylinder membranes showed reasonable preservation in KMnO4 and excellent preservation in OsO4. The widths of membranes after treatment with these two fixatives was 3 m and 2 m respectively. 相似文献
106.
Roger Höglund 《Cell and tissue research》1967,76(4):568-576
Summary Carotid body tissue from horse and dog has been investigated ultrastructurally. Several cell types are recognized: glomus cells which are regarded as chemoreceptors, sustentacular cells which enclose the glomus cells, and nerve fibers.The glomus cells contain electron dense granules which are interpreted as packages of biogenic monoamines. There are both dark and light glomus cells, the former containing more granules and ribosomes. Invaginations of the plasma membranes as well as free coated vesicles are often seen in the cytoplasm of glomus cells. Nerves within the glomus lobules are generally wrapped by sustentacular cells, but nerve endings are also seen in close contact with the glomus cells. Some endings contain synaptic vesicles as well as a great concentration of mitochondria. The corresponding fibers are thought to be efferent. Another type of contact is interpreted as en passant synapses of afferent fibers.The author wishes to express his gratitude to Professor L. Nicander who initiated this project and took most of the micrographs and to Professor Nils Obel and associate Professor Gustav Björk at the Royal Veterinary College for their valuable help with the surgical procedure and to Dr. Martin Ritzén of the Royal Medical College for making the tests for biogenic monoamines. 相似文献
107.
108.
109.
110.
Michael G. Ritchie Roger K. Butlin Godfrey M. Hewitt 《Journal of evolutionary biology》1989,2(5):339-352
Two subspecies of the grasshopper Chorthippus parallelus meet and form a hybrid zone in the Pyrenees. Both strong hybrid dysfunction and a behavioural difference occur, which would seem to make the zone a suitable candidate for speciation by reinforcement. One of the classic ways of looking for this is to test for increased levels of assortative mating between populations from close to the region of contact. Here we show that, with virgin insects, such assortment decreases as one approaches the centre of the zone in C. parallelus. The pattern is different upon remating, with non-virgins showing a pattern more like that predicted by reinforcement. Overall there is little evidence for reinforcement. We argue that other tests of the model may be more appropriate for stable hybrid zones. 相似文献