全文获取类型
收费全文 | 214篇 |
免费 | 10篇 |
国内免费 | 3篇 |
出版年
2023年 | 1篇 |
2021年 | 2篇 |
2020年 | 4篇 |
2019年 | 3篇 |
2018年 | 3篇 |
2017年 | 3篇 |
2016年 | 6篇 |
2015年 | 10篇 |
2014年 | 16篇 |
2013年 | 13篇 |
2012年 | 21篇 |
2011年 | 24篇 |
2010年 | 15篇 |
2009年 | 12篇 |
2008年 | 11篇 |
2007年 | 10篇 |
2006年 | 3篇 |
2005年 | 6篇 |
2004年 | 5篇 |
2003年 | 1篇 |
2001年 | 1篇 |
2000年 | 3篇 |
1999年 | 7篇 |
1998年 | 1篇 |
1996年 | 1篇 |
1993年 | 1篇 |
1992年 | 2篇 |
1991年 | 1篇 |
1990年 | 2篇 |
1989年 | 6篇 |
1988年 | 1篇 |
1987年 | 5篇 |
1986年 | 3篇 |
1984年 | 1篇 |
1981年 | 2篇 |
1980年 | 3篇 |
1979年 | 1篇 |
1977年 | 2篇 |
1975年 | 1篇 |
1974年 | 2篇 |
1973年 | 3篇 |
1972年 | 1篇 |
1966年 | 1篇 |
1965年 | 1篇 |
1964年 | 1篇 |
1935年 | 1篇 |
1929年 | 1篇 |
1927年 | 1篇 |
1924年 | 1篇 |
1911年 | 1篇 |
排序方式: 共有227条查询结果,搜索用时 78 毫秒
101.
102.
A new repetitive element of the CR1 family downstream of the chicken vitellogenin gene. 总被引:5,自引:2,他引:3
We have analyzed a repetitive DNA sequence found in the 3'-flanking region of the chicken vitellogenin gene. By its sequence, the repetitive DNA has been identified as a hitherto unreported member of the chicken CR1 family of repetitive elements. The CR1 sequence displays the structural characteristics of a long terminal repeat located at the 3' end of an avian retrovirus. The CR1 element lies 2.2 kb downstream of the vitellogenin gene and 'points' away from the gene rather than toward it. In this respect, this element differs from other CR1 repeats. The CR1 element is embedded in a region showing changes in chromatin structure implying a potential role for this sequence in determining the structural state of the local chromatin. 相似文献
103.
104.
K. N. Faber S. Westra H. R. Waterham I. Keizer-Gunnink W. Harder G. AB M. Veenhuis 《Applied microbiology and biotechnology》1996,45(1-2):72-79
We describe the synthesis and purification of two functional peptides, namely human insulin-like growth factor II (IGF-II)
and Xenopus laevis magainin II in Hansenula polymorpha after their synthesis as hybrid proteins fused to the C terminus of endogenous amine oxidase. The hybrid genes, placed under
control of the H. polymorpha alcohol oxidase promoter (PAOX), were integrated into the genomic alcohol oxidase locus, yielding stable production strains. High-level synthesis of the
fusion proteins, exceeding 20% of total cellular protein, was obtained when the transformed strains were grown in methanol-limited
chemostat cultures; when expressed by itself, i.e. in the absence of the amine oxidase gene, IGF-II could not be recovered
from crude cell extracts, probably as a result of rapid proteolytic degradation. Accumulation in peroxisomes did not significantly
affect the IGF-II protein stability when expressed in the absence of the carrier protein. Apparently, fusion to the large
(±78 kDa) amine oxidase carrier particularly stabilizes the peptides and prevents them from proteolysis. After partial purification,
the fusion partners were readily separated by factor Xa treatment.
Received: 16 June 1995 / Accepted: 20 September 1995 相似文献
105.
S Jahan S Singh A Srivastava V Kumar D Kumar A Pandey CS Rajpurohit AR Purohit VK Khanna AB Pant 《Molecular neurobiology》2018,55(4):2828-2839
106.
Effect of ML-236B (compactin) on biliary excretion of bile salts and lipids, and on bile flow, in the rat 总被引:2,自引:0,他引:2
H J Kempen J De Lange M P Vos-Van Holstein P Van Wachem R Havinga R J Vonk 《Biochimica et biophysica acta》1984,794(3):435-443
To assess the importance of de novo cholesterol synthesis for bile salt formation, the effects of ML-236B (an inhibitor of 3-hydroxy-3-methylglutaryl-coenzyme A reductase) on biliary excretion of bile salts and lipids were studied in rats with permanent catheters in bile duct, heart and duodenum. In rats having their bile diverted continuously for 8 days, duodenal administration of ML-236B (50 mg/kg) caused an immediate transient choleresis, which subsided after 2 h. Concomitant with the choleresis concentrations of bile salt, phospholipid and cholesterol fell, but this decrease was maintained for 6 h. Consequently, ML-236B inhibited biliary output salts and lipids from the second till the sixth hour after injection. The kinetics of biliary excretion of intravenously injected [14C]taurocholate were not affected by ML-236B administration. In rats having their biliary catheter connected to the duodenal catheter, or in rats with prolonged bile diversion but treated with mevalonolactone, ML-236B again caused a transient choleresis (having subsided after 2 h), but now did not affect biliary excretion of bile salts and lipids. It is concluded that (1) ML-236B causes a transient bile salt-independent choleresis, (2) ML-236B depresses excretion of bile salts and lipids by blocking mevalonate synthesis and not by blocking the bile salt or lipid transport, (3) biliary excretions of phospholipids and cholesterol partly depend on excretion of bile salt, and (4) in rats with a prolonged total bile diversion newly formed mevalonate is a major substrate for bile salt synthesis. 相似文献
107.
We describe a new scaffold-free three-dimensional (3D) cell culture model using cholesteryl ester based lyotropic liquid crystal (LC) substrates. Keratinocytes were deposited randomly on the LC surface where they self-assembled into 3D microtissues or keratinospheroids. The cell density required to form spheroids was optimized. We investigated cell viability using dead/live cell assays. The adhesion characteristics of cells within the microtissues were determined using histological sectioning and immunofluorescence staining. Fourier transform infrared spectroscopy (FTIR) was used to characterize the biochemistry of the keratinospheroids. We found that both cells and microtissues could migrate on the LC surface. The viability study indicated approximately 80% viability of cells in the microtissues up to 20 days of culture. Strong intercellular adhesion was observed in the stratification of the multi-layered microspheroids using field emission-scanning electron microscopy (FE-SEM) and histochemical staining. The cytoskeleton and vinculins of the cells in the microtissues were expressed diffusely, but the microtissues were enriched with lipids and nucleic acids, which indicates close resemblance to the conditions in vivo. The basic 3D culture model based on LC may be used for cell and microtissue migration studies in response to cytochemical treatment. 相似文献
108.
109.
Siti Mutripah Maria Dyah Nur Meinita Ji-Young Kang Gwi-Taek Jeong AB Susanto Romanus Edy Prabowo Yong-Ki Hong 《Journal of applied phycology》2014,26(1):687-693
Seaweeds, particularly species of red macroalgae, are promising resources for bioethanol production because of their exceptionally high carbohydrate content. Of 20 seaweeds evaluated, Palmaria palmata (Rhodymenia palmata) contained the highest carbohydrate content (469.8 mg g?1 seaweed) with a carrageenan content of 354 mg g?1 seaweed. Such a high carrageenan content makes the high-volume production of bioethanol feasible. Acid hydrolysis of P. palmata in 0.4 M H2SO4 at 125 °C for 25 min released 27 mg of glucose, 218.4 mg of reducing sugars, and 127.6 mg of galactose per gram of seaweed. Ethanol fermentation of these hydrolysis products using an inoculum concentration of 1.5 mg mL?1 at 30 °C and 72 h in a shaking incubator at 130 rpm yielded 17.3 mg of ethanol per gram of seaweed. 相似文献
110.
A new species of marine interstitial wormshrimp, Ingolfiella
maldivensis, is described from coral sand on the inner and outer reef off Magoodhoo island, Faafu atoll, Maldives. Six females were found and compared to other species from the Maldives and those bordering the Indian Ocean and beyond. Morphological resemblance ties it to a species from the Caribbean island of Curaçao. Both species are found in shallow sublittoral interstitial spaces. 相似文献