全文获取类型
收费全文 | 2315篇 |
免费 | 162篇 |
专业分类
2477篇 |
出版年
2016年 | 32篇 |
2015年 | 33篇 |
2014年 | 39篇 |
2013年 | 126篇 |
2012年 | 78篇 |
2011年 | 82篇 |
2010年 | 61篇 |
2009年 | 55篇 |
2008年 | 84篇 |
2007年 | 84篇 |
2006年 | 78篇 |
2005年 | 82篇 |
2004年 | 100篇 |
2003年 | 105篇 |
2002年 | 94篇 |
2001年 | 43篇 |
2000年 | 55篇 |
1999年 | 33篇 |
1998年 | 26篇 |
1997年 | 21篇 |
1996年 | 33篇 |
1994年 | 30篇 |
1993年 | 33篇 |
1992年 | 61篇 |
1991年 | 44篇 |
1990年 | 22篇 |
1989年 | 40篇 |
1988年 | 31篇 |
1987年 | 32篇 |
1986年 | 37篇 |
1985年 | 32篇 |
1984年 | 29篇 |
1983年 | 36篇 |
1981年 | 26篇 |
1980年 | 26篇 |
1979年 | 29篇 |
1978年 | 27篇 |
1977年 | 27篇 |
1976年 | 28篇 |
1975年 | 21篇 |
1974年 | 40篇 |
1973年 | 27篇 |
1972年 | 36篇 |
1971年 | 33篇 |
1970年 | 17篇 |
1968年 | 24篇 |
1967年 | 24篇 |
1966年 | 25篇 |
1965年 | 16篇 |
1961年 | 19篇 |
排序方式: 共有2477条查询结果,搜索用时 15 毫秒
931.
932.
933.
Nutrient enrichment enhances hidden differences in phenotype to drive a cryptic plant invasion 总被引:1,自引:0,他引:1
Christine Holdredge Mark D. Bertness Eric Von Wettberg Brian R. Silliman 《Oikos》2010,119(11):1776-1784
Many mechanisms of invasive species success have been elucidated, but those driving cryptic invasions of non‐native genotypes remain least understood. In one of the most successful cryptic plant invasions in North America, we investigate the mechanisms underlying the displacement of native Phragmites australis by its Eurasian counterpart. Since invasive Phragmites’ populations have been especially prolific along eutrophic shorelines, we conducted a two‐year field experiment involving native and invasive genotypes that manipulated nutrient level and competitor identity (inter‐ and intra‐genotypic competition) to assess their relative importance in driving the loss of native Phragmites. Inter‐genotypic competition suppressed aboveground biomass of both native and invasive plants regardless of nutrient treatment (~ 27%), while nutrient addition disproportionately enhanced the aboveground biomass (by 67%) and lateral expansion (by > 3 × farther) of invasive Phragmites. Excavation of experimental plots indicated that nutrient addition generates these differences in aboveground growth by differentially affecting rhizome production in invasive vs native plants; invasive rhizome biomass and rhizome length increased by 595% and 32% with nutrient addition, respectively, while natives increased by only 278% and 15%. Regardless of nutrient level, native rhizomes produced twice as many roots compared to invasives, which field surveys revealed are heavily infected with mycorrhizal symbionts. These results suggest that native Phragmites competes well under nutrient‐limited conditions because its rhizomes are laden with nutrient‐harvesting roots and mycorrhizae. Invasive Phragmites’ vigorous aboveground response to nutrients and scarcity of lateral roots, in contrast, may reflect its historic distribution in eutrophic Eurasian wetlands and correspond to its prevalence in New England marshes characterized by elevated nutrient availability and relaxed nutrient competition. These findings reveal that discrete differences in phenotype can interact with anthropogenic modification of environmental conditions to help explain the success of cryptic invaders. 相似文献
934.
Prof. Dr L. Von Ubisch 《Acta biotheoretica》1943,7(3-4):163-182
Summary Great theoretical value has always been attached toBoveri's discovery as regards chromatin diminution inAscaris, for this discovery appeared to expose the mechanism causing the propagative cells, in which all chromatin remains, to originate an entirely new organism, whereas the soma cells of which the chromosomes have been diminished are only capable of specific differentiation.Boveri was further able to show that, not only do the soma and propagative nuclei differ from each other, but that rather the character of the cell-plasma decides whether the nucleus diminishes or not. Considerations are brought forward to prove that the diminisher hypothesis as given byKing andBeams, is untenable. Diminution has always been supposed to result in the loss of the totipotence of the diminished nuclei. In the light of recent chromosome research this course of events does not seem probable. Therefore the following theory is brought forward: When the egg ofAscaris is split, blastomeres originate, which differ from each other as to the plasma. In proportion to the quality of the surrounding plasma, now these now other genes of the totipotent nucleus react in different cells, thus originating the specific differentiation of the cell in question. This cooperation between plasma and nucleus is, however, at first obstructed in the large collective chromosomes ofAscaris, by the presence of their thickened ends. In order to keep up this cooperation a certain quality of plasma is needed, situated in a certain horizontal zone of the egg. As soon as, in the course of the division, nuclei come to rest in different zones, a diminution occurs, and differentiation of the cells in question sets in. Towards the end of the embryonic development the ends of the chromosomes are lost in the propagative cells, and also in these cells differentiation occurs.
Résumé On a toujours attribué une haute signification théorique à la découverte deBoveri, de la diminution des chromatines chez l'ascaris puisqu'elle semblait expliquer le mécanisme qui cause que les cellules propagatrices où séjourne, la totalité de l'effectif des chromatines, font de nouveau sortir d'elles-mêmes un organisme entier, pendant que les cellules somatiques, dont les chromosomes ont été diminués, ne sont plus capables qu'à produire une différenciation spécifique. De plus,Boveri a pu démontrer, que les noyaux somatiques et propagateurs ne sont pas, à peu de chose près, différents, mais que c'est bien plus la qualité du plasme des cellules qui décide si les noyaux qui leur ont été distribués, diminuent ou non. Des arguments sont apportés, qui démontrent que l'hypothèse du diminisher échafaudée parKing etBeams est insoutenable. En général on s'est représenté. l'action de la diminution de telle façon que la totipotentialité des noyaux diminués a été perdue. Cependant, le fait n'est pas acceptable quand on le considère à la lumière de la recherche moderne sur les chromosomes. Par conséquent, nous proposons l'opinion suivante: Dans l'uf de l'ascaris, des blastomères différant plasmatiquement les uns des autres prennent naissance pendant le fendage. Selon l'état du plasme environnant, dans les cellules individuelles on voit réagir certain gène, dans les autres un autre gène du noyau totipotentiel, causant ainsi la différenciation des cellules visées. Cette collaboration entre le plasme et le noyau est cependant premièrement bloquée dans les grands chromosomes collectifs de l'ascaris par la présence des bouts de chromosomes épaissis. Pour leur conservation une certaine qualité de plasme est requise qui se trouve dans une zone horizontale déterminée de l'uf. Dès que, dans le procès du fendage, des noyaux arrivent dans d'autres zones du plasme, la diminution a lieu, et la différenciation des cellules visées s'opère. Cependant, après la conclusion du développement embryonal, les bouts des chromosomes se perdent dans les cellules propagatrices et la différenciation a lieu aussi dans ces cellules.相似文献
935.
E I C F Von Ledebur J P Almeida E S Loss G F Wassermann 《Hormones et métabolisme》2002,34(10):550-555
For this study, we investigated the changes in the electrophysiological parameters of Sertoli cells in seminiferous tubules from 17 - 19 day-old rats induced by testosterone. Using conventional intracellular microelectrode techniques, we analysed the membrane potential and its input resistance. The entire tubules were fixed in a superfusion chamber continuously perfused with Krebs-Ringer bicarbonate buffer (pH 7.4, 32 degrees C). Visual control of cell impalement was achieved using an inverted microscope. The parameters analysed were passed through an amplifier and recorded using a proprietary software system. The topical application of testosterone (0.1 to 10 microM) led to an immediate (within 30 seconds) and significant dose-dependent depolarization of the membrane potential of the cell at all concentrations used. Concomitantly, the input resistance of the cell membrane underwent a significant increment at 30 seconds. These changes returned to resting values after washout. Topical administration of 17beta-estradiol or progesterone (10 microM) did not change the membrane potential. The addition of the K +ATP channel agonist diazoxide to the perfusion buffer nullified the depolarization effect of testosterone at 30 seconds. This result suggests that the immediate action of testosterone is associated with the closing of K +ATP channels, thereby depolarizing the membrane. 相似文献
936.
The possible involvement of protein phosphatase 1 in thrombin-induced Ca2+ influx of human platelets
Kouhei Murata Masato Sakon Jun-Ichi Kambayashi Masao Yukawa Yoshiko Yano Kazumasa Fujitani Tomio Kawasaki Eiichi Shiba Takesada Mori 《Journal of cellular biochemistry》1993,51(4):442-445
Protein phosphatase 1 is considered to be involved in thrombin-induced platelet activation (Murata et al., Biochem Int 26:327–334, 1992). To clarify the mechanism, we examined the effects of protein phosphatase 1 and 2A inhibitors (calyculin A, tautomycin, okadaic acid) on Ca2+ influx. In the presence of 1 mM Ca2+, thrombin- (0.1 U/ml) induced platelet aggregation and ATP release were inhibited by calyculin A, while this inhibitory effect was abolished in the absence of Ca2+ (EGTA 1 mM). Furthermore, thrombin-induced Mn2+ influx but not intracellular Ca2+ mobilization was inhibited by calyculin A in a dose-related manner. Calyculin A also blocked the ongoing Ca2+ influx when added 3 min after thrombin stimulation. Similar inhibitory effects were observed with okadaic acid and tautomycin in the same potency sequence as the reported one for protein phosphatase 1 (calyculin A > tautomycin > okadaic acid). These results suggest that the anti-platelet effects of phosphatase inhibitors are due to the inhibition of Ca2+ influx and that protein phosphatase 1 plays a key role in the regulation of receptor operated Ca2+ channel of human platelets. 相似文献
937.
Neutral mutation and evolutionary progress The process and causes of regressive evolution are still under debate. Contrary to DARWIN'S original assumption, Neo-Darwinian proponents make selection responsible for reduction. Biologically functionless structures like eye and pigmentation in cave animals deliver excellent material to study this problem. Comparison of regressive (eye, pigmentation, aggression, dorsal light reaction) and constructive traits (gustatory equipment, egg yolk content, feeding behavior) in epigean and cave fish (Astyanax fasciatus, Characidae) reveal a high variability of the regressive features in the cave forms. Contrary to this, the constructive traits are characterized by a low variability in epigean and cave fish. This difference is attributed to the lack of selection on regressive structures. The existence of an intermediate cave population between epigean and true cave fish of A. fasciatus makes possible the study of evolutionary rates. It is shown that the regressive traits do not evolve more quickly than the constructive ones do. On the contrary, constructive traits like egg yolk content are even more rapid because they are of great biological value in the cave biotope. Especially energy economy is claimed by Neo-Darwinists to play a decisive role as a selective force. Comparison of the development of epi- and hypogean larvae of A. fasciatus shows that the formation of a smaller and less differentiated eye in the cave specimens has no effect on body growth. Furthermore, even behavioral traits like aggressiveness, schooling, dorsal light reaction, or negative phototaxis, which all are not performed in darkness by the epigean ancestor, become genetically reduced in the cave fish. The principles of regressive evolution, loss of selection and increase in variability, play a central role in evolution in general. When biota with empty niches are colonized, stabilizing selection relaxes from the special adaptations to the niche inhabited before by the invading species. Variability may arise in these and is permitted as long as fitness is guaranteed. Such processes characterize adaptive radiation. Examples are given by the species flocks on isolated islands or in chemically abnormal lakes like those of the East African Rift Valley. Only secondarily, on the basis of the arisen variability, does directional selection promote the newly developing species into different niches. The loss of stabilizing selection is an important factor for the evolutionary process to be open for evolutionary progress. 相似文献
938.
939.
H. Brandstetter R. A. Engh E. G. Von Roedern L. Moroder R. Huber W. Bode F. Grams 《Protein science : a publication of the Protein Society》1998,7(6):1303-1309
Matrix metalloproteinases (MMPs) are a family of zinc endopeptidases, which have been implicated in various disease processes. Various classes of MMP inhibitors, including hydroxamic acids, phosphinic acids, and thiols, have been previously described. Most of these mimic peptides, and most likely bind analogous to the corresponding peptide substrates. Among the hydroxamic acids, malonic acid derivatives have been used as MMP inhibitors, although optimization of their inhibition potency was not successful. Here we report the design of malonic acid-based inhibitors using the X-ray structure of a collagenase/inhibitor complex, which revealed a nonsubstrate-like binding mode. The proposed beta-type turn-like conformation for the improved inhibitors was confirmed by X-ray crystallography. The observation of nonsubstrate-like binding confirms the original strategy for structure-based modeling of improved malonic acid inhibitors, and explains kinetic data that are inconsistent with substrate-like binding. Detailed interactions for the improved inhibitors seen in the crystal structure also suggest possibilities for further modifications in cycles of structure based drug design. Indeed, we have designed nonpeptidic inhibitors with approximately 500-fold improved inhibition based on these structures. 相似文献
940.
Kazuo Yamaguchi Eiichi Fuse Michio Takashima Tohru Yasuzawa Takashi Kuwabara Satoshi Kobayashi 《Journal of chromatography. B, Analytical technologies in the biomedical and life sciences》1998,713(2):887
A sensitive method for the determination of an anti-cancer agent, DX-52-1 (7-cyanoquinocarcinol, I) and quinocarmycin (II) which is formed from I either by metabolism or degradation, in human plasma has been developed utilising liquid chromatography electrospray–ionization tandem mass spectrometry (LC–ESI-MS–MS). The procedure involves solid-phase extraction at pH 2 and low temperature (4–6°C) to prevent the decomposition of I to II, the separation by reversed-phase HPLC and the multiple reaction monitoring (MRM) by ESI-MS–MS. The mean precision and accuracy at the lower limit of quantitation (LLOQ) of I, 0.25 ng ml−1, were 8.7% and −10.8%, respectively. Since an interfering peak eluting slightly earlier than II was observed on the HPLC of blank plasma, the LLOQ of II was set at 5 ng ml−1 where the mean precision and accuracy were 15.6% and −9.8%. The results suggested that the method is useful for the simultaneous monitoring of Iand II in the clinical trials of I. 相似文献