Argiopinin-binding proteins from the membrane of the bull cerebral cortex

The 40 kDa argiopinin-binding glycoprotein has been isolated from the solubilised preparations of bovine cerebrum membranes by means of two-step biospecific chromatography on affinity sorbents with immobilized glutamate and argiopinins. This receptor component displays a specific L-[3H]glutamate binding with Kd = 0.18 +/- +/- 0.019 mumole and Bmax = 43 +/- 4.5 nmole/mg. Amino acid analysis reveals it to be a member of integral membrane proteins.     

Adaptation of Bio-Layer Interferometry for Quantitative Assessment of the Vascular Endothelial Growth Factor Content in Cell-Conditioned Culture Medium

Biophysics - Quantitative determination of cytokines, chemokines, growth factors, and other soluble protein molecules in various biological fluids is a routine task of modern diagnostics and...     

Method for determining kallikrein of tissue origin in blood plasma and its clinical significance

Based on a study of the kininogenase activity of the total plasma kallikrein in the presence of 3 concentrations of the soybean inhibitor trypsin (0.5, 1.0, 10.0 micrograms/ml) one can measure at a time the activity of tissue kallikrein (without specifying the source) and the activity of 3 forms of plasma kallikrein, including its adsorption on kaolin that characterizes the conformational structure of the enzyme. Examination of 10 healthy subjects and 136 patients revealed a 10 to 20-fold increase in the content of tissue kallikrein in plasma of 70% of diabetes mellitus patients and a 2.5 to 3-fold elevation in 50% of patients with chronic occupational bronchitis, and in 30% of patients suffering from chronic hepatitis. The method suggested makes it possible to have a better insight into the physiological and pathogenetic role of the kinin system and may be used for laboratory control over the treatment efficacy.     

Morphogenesis and histophysiology of the corpus luteum

A great amount of different problems on morphogenesis and histophysiology of the corpus luteum is presented, with an emphasis on light optic and ultrastructural data that characterize the developmental dynamics of the corpus luteum. The vascular reaction is described in details, beginning from the preovulatory period. The total high vascularization rate is demonstrated and certain information on ultrastructure of newly formed capillaries and macrophages is concerned with. For the first time the authors' data on intravascular macrophages are given. The role of macrophages in the function and structural dynamics of the corpus luteum is discussed. Owing to the results obtained histochemically, ultrastructurally and biochemically, the subject on dynamics of the corpus luteum hormonoproduction, on processes participating in the hormone secretion, as well as on the role of the interstitial tissue in the corpus luteum formation is considered. The data from the literature and those of the authors are presented concerning the means and ways of progesteron transport in the form of vesicles, granules, or by means of molecular diffusion. Participation of the corpus luteum macrophages (tissue and vascular ones) in processes of synthesis and transport of progesteron is analysed. The role of prostaglandins in the chain of regulation of development, function and involution of the corpus luteum is studied. The changes in balance of prostaglandins, when prostaglandin F2 is administered result in decreasing amount of progesterone in blood. In the experiment, synthesis of prostaglandins is blocked by indometacin administration and it causes certain disturbances in luteal transformation.