Proliferation of piriform neurons in the rat cerebellar cortex in the pre- and postnatal periods of development]

The method of histoautoradiography with the use of H3-thymidine was applied to the study; there were established the periods of appearance in the cerebellar anlage of albino rats of neuroblasts differentiated into the piriform neurones of the cerebellar cortex; dynamics of the proliferative activity of these cellular elements in the course of the pre- and postnatal periods of development of the experimental animals was investigated. On the basis of the material obtained a conclusion was drawn that the last cell divisions (the result of cell differentiation were Purkinje's cells of the cerebellar cortex) stopped by the 13th--15th day of the embryonic development. No incorporation of the labeled precursor into the DNA of the nuclei of the differentiating piriform neurons occurred later.     

Relationship between Neutrophins and Gut Microbiome in Various Metabolic Types of Obesity

Journal of Evolutionary Biochemistry and Physiology - Obesity is associated with a gut microbiome imbalance and the risk of neurological complications. However, the risk of complications is...     

Erratum to: Effect of the Entomopathogenic Fungus Beauveria bassiana on the Development of Faba Bean (Vicia faba) Diseases in the Field Conditions

Doklady Biochemistry and Biophysics - An Erratum to this paper has been published: https://doi.org/10.1134/S1607672922330018     

Cell mechanisms for apoptosis induction in K562 human erythroleukemia cell line treated with quinoline-N-oxide derivatives

The effect of two quinoline-N-oxide derivatives (2-(4′-nitrostyryl)-quinoline-l-oxide (2-NSQO) and 4-(4′-nitrostyryl)-quinoline-1-oxide (4-NSQO)) on modulation of microsomal NADPH oxidoreductase activity, nicotinamide coenzyme concentrations, and induction of apoptosis in K562 human erythroleukemia cells has been. 4-NSQO at the concentration of (10 μM) and 2-NSQO (10 μM) inhibited the activity of microsomal NADPH cytochrome c reductases in tumor cells by 15 and 50% respectively. Treatment of cells with these compounds for two days resulted in the activation of caspase-9 and caspase-3, the increase in ethidium bromide and 4′,6-diamidino-2-phenylindole (DAPI) fluorescence upon DNA binding, and induction of apoptosis. The latter was preceded by the decrease in intracellular nicotinamide coenzyme concentrations. The results obtained allow considering 4-NSQO (and its structural analogs) as perspective compounds for further experimental studies as antitumor agent with low toxic effect on tissues of an organism.     

Full genomic sequence of the prototype strain (M64) of Rio Bravo virus

Rio Bravo virus (RBV) is a member of the family Flaviviridae, genus Flavivirus. It belongs to a group of viruses in the genus with no known vector. In this report, we analyze the complete genome of the prototype RBV, strain M64.     

Structure of replication initiation region in <Emphasis Type="Italic">Pseudomonas</Emphasis> IncP-7 streptomycin resistance plasmid Rms148

Pseudomonas’ IncP-7 plasmids play significant role in the environmental biodegradative potential and sometimes carry antibiotic-resistance genes. Rms148 plasmid was used as archetypal P-7 plasmid in microbiological incompatibility studies for more then 30 years. However, the structure of its basic replicon was not described until now; furthermore, the phylogenetic relationships between all known plasmids within the IncP-7 group have not yet been studied. In the course of our study, we have constructed two pairs of primers to amplify the main components of the region of the initiation of P-7 replication, and the subsequent screening of repA intragenic polymorphism was performed using the laboratory collection of IncP-7 plasmids. The minimal replicon of Rms148 was determined and its nucleotide sequence was found to be 81–83% identical to repA-oriV of known P-7 plasmids and is considered to fall into a separate clade of the corresponding phylogenetic tree. Additionally, repA group members seem to be more conservative than the putative oriV region. The estimated amino acid sequence and predicted secondary and tertiary structures of Rms148 RepA protein allowed us to make the assumption that the initiation of replication in plasmids of the P-7 incompatibility group is described by the same model as for the unclassified cryptic plasmid pPS10.     

Synthesis of novel МТ3 receptor ligands via an unusual Knoevenagel condensation

New 5-acetamido-substituted melatonin derivatives were efficiently synthesized in excellent yields via Knoevenagel condensation.The relative binding affinity of new synthesized compounds to MT3 receptor was tested via enzymatic assays and the X-ray structures of the most potent compounds were determined in complex with MT3.