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Borgoyakova M. B. Karpenko L. I. Rudometov A. P. Shanshin D. V. Isaeva A. A. Nesmeyanova V. S. Volkova N. V. Belenkaya S. V. Murashkin D. E. Shcherbakov D. N. Volosnikova E. A. Starostina E. V. Orlova L. A. Danilchenko N. V. Zaikovskaya A. V. Pyankov O. V. Ilyichev A. A. 《Molecular Biology》2021,55(6):889-898
Molecular Biology - The development of preventive vaccines became the first order task in the COVID-19 pandemic caused by SARS-CoV-2. This paper reports the construction of the pVAX-RBD plasmid... 相似文献
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Carmen Benítez-Benítez Santiago Martín-Bravo Charlotte S. Bjorå Sebastian Gebauer rew L. Hipp Matthias H. Hoffmann Modesto Luceño Tyril M. Pedersen Anton Reznicek Eric Roalson Polina Volkova Okihito Yano Daniel Spalink Pedro Jiménez-Mejías 《植物分类学报:英文版》2021,59(4):642-667
Carex section Phacocystis (Cyperaceae) is one of the most diverse and taxonomically complex groups of sedges (between 116 and 147 species), with a worldwide distribution in a wide array of biomes. It has a very complicated taxonomic history, with numerous disagreements among different treatments. We studied the biogeography and niche evolution in a phylogenetic framework to unveil the relative contribution of geographical and ecological drivers to diversification of the group. We used a large species sampling of the section (82% of extant species) to build a phylogeny based on four DNA regions, constrained with a phylogenomic HybSeq tree and dated with six fossil calibrations. Our phylogenetic results recovered section Phacocystis s.s. (core Phacocystis) as sister to section Praelongae. Ancestral area reconstruction points toward the N Pacific as the cradle for the crown diversification of section Phacocystis during the Middle Miocene. Wide distributions were recurrently inferred across deep nodes. Large Northern Hemisphere lineages with geographical congruence were retrieved, pointing toward the importance of allopatric divergence at deep phylogenetic levels, whereas within-area speciation emerges as the predominant pattern at shallow phylogenetic level. The Southern Hemisphere (Neotropics, SW Pacific) was colonized several times from the Northern Hemisphere. The global expansion of Carex section Phacocystis did not entail major ecological changes along the inner branches of the phylogeny. Nevertheless, ecological differentiation seems to gain importance toward recent times. 相似文献
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Volkova L. A. Urmantseva V. V. Burgutin A. B. Nosov A. M. 《Russian Journal of Plant Physiology》2021,68(6):1098-1106
Russian Journal of Plant Physiology - The effect of exogenous furostanol glycosides (FG) on the activity of redox enzymes was investigated in suspension cell culture of alfalfa (Medicago sativa... 相似文献
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L. A. Volkova V. V. Urmantseva A. B. Burgutin A. M. Nosov 《Russian Journal of Plant Physiology》2013,60(2):235-243
Biological activity of the extract from golden root (Rhodiola rosea L.) roots, containing the complex of phenylpropanoids (CPP), was studied on the cell culture of yam (Dioscorea deltoidea Wall) under normal conditions and abiotic stress. The high radical-binding capacity of CPP relative to anion- and hydroxyl-radicals was observed. Having a high level of antiradical protection, CPP at a high concentration(100 μM) exerted prooxidant effect, causing a decrease in D. deltoidea cell viability and a decrease in the activities of antioxidant enzymes: superoxide dismutase, guaiacol-dependent peroxidase, and catalase, with the exception of ascorbate peroxidase. At treatment with 100 μM CPP, oxidase (prooxidant) activity of peroxidase increased by three times. The low CPP concentration (2 μM) did not induce substantial changes in the activities of tested enzymes and also a substantial increase in the oxidase activity of peroxidase. Under conditions of oxidative stress induced by paraquat and high temperature, CPP manifested adaptogenic action, increasing cell viability; however, under hyperosmotic stress, it was not efficient. CPP was most efficient at a low concentration after cell pre-incubation with it for 5 days. In this case, the amount of primary and secondary POL products increased. Shortening pre-cultivation with CPP reduced its defensive effect. 相似文献
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Yahya Sohrabi Helena Havelková Tetyana Kobets Matyá? ?íma Valeriya Volkova Igor Grekov Ta?ána Jaro?íková Iryna Kurey Jarmila Vojtí?ková Milena Svobodová Peter Demant Marie Lipoldová 《PLoS neglected tropical diseases》2013,7(7)
Background
L. tropica can cause both cutaneous and visceral leishmaniasis in humans. Although the L. tropica-induced cutaneous disease has been long known, its potential to visceralize in humans was recognized only recently. As nothing is known about the genetics of host responses to this infection and their clinical impact, we developed an informative animal model. We described previously that the recombinant congenic strain CcS-16 carrying 12.5% genes from the resistant parental strain STS/A and 87.5% genes from the susceptible strain BALB/c is more susceptible to L. tropica than BALB/c. We used these strains to map and functionally characterize the gene-loci regulating the immune responses and pathology.Methods
We analyzed genetics of response to L. tropica in infected F2 hybrids between BALB/c×CcS-16. CcS-16 strain carries STS-derived segments on nine chromosomes. We genotyped these segments in the F2 hybrid mice and tested their linkage with pathological changes and systemic immune responses.Principal Findings
We mapped 8 Ltr (Leishmania tropica response) loci. Four loci (Ltr2, Ltr3, Ltr6 and Ltr8) exhibit independent responses to L. tropica, while Ltr1, Ltr4, Ltr5 and Ltr7 were detected only in gene-gene interactions with other Ltr loci. Ltr3 exhibits the recently discovered phenomenon of transgenerational parental effect on parasite numbers in spleen. The most precise mapping (4.07 Mb) was achieved for Ltr1 (chr.2), which controls parasite numbers in lymph nodes. Five Ltr loci co-localize with loci controlling susceptibility to L. major, three are likely L. tropica specific. Individual Ltr loci affect different subsets of responses, exhibit organ specific effects and a separate control of parasite load and organ pathology.Conclusion
We present the first identification of genetic loci controlling susceptibility to L. tropica. The different combinations of alleles controlling various symptoms of the disease likely co-determine different manifestations of disease induced by the same pathogen in individual mice. 相似文献18.
Abstract This study reports rate-of-dialysis of an iodinated N-methyl-D-aspartate antagonist drug, [125-1] MK-801, from solutions of lipid vesicles and from proteoliposomes containing purified membrane proteins. A 170 kd protein precipitated from proteoliposomes cross reacts with monoclonal antibodies against cloned NMDA-NR2(A) and NR2(B) subunits. Drug binding in proteoliposomes includes contributions from lipid and from protein, in addition to lipid. A significant change in drug binding was observed in proteoliposomes in response to 10 uM agonist, NMDA. Rate-of-dialysis from agonist-stimulated proteoliposomes was sensitive to perturbation by decreased aqueous ligand concentration in a manner consistent with a lipid-mediated receptor/antagonist equilibrium. 相似文献
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Alexander G. Volkov Chrystelle L. Vilfranc Veronica A. Murphy Colee M. Mitchell Maia I. Volkova Lawrence O’Neal Vladislav S. Markin 《Journal of plant physiology》2013
The electrical phenomena and morphing structures in the Venus flytrap have attracted researchers since the nineteenth century. We have observed that mechanical stimulation of trigger hairs on the lobes of the Venus flytrap induces electrotonic potentials in the lower leaf. Electrostimulation of electrical circuits in the Venus flytrap can induce electrotonic potentials propagating along the upper and lower leaves. The instantaneous increase or decrease in voltage of stimulating potential generates a nonlinear electrical response in plant tissues. Any electrostimulation that is not instantaneous, such as sinusoidal or triangular functions, results in linear responses in the form of small electrotonic potentials. The amplitude and sign of electrotonic potentials depend on the polarity and the amplitude of the applied voltage. Electrical stimulation of the lower leaf induces electrical signals, which resemble action potentials, in the trap between the lobes and the midrib. The trap closes if the stimulating voltage is above the threshold level of 4.4 V. Electrical responses in the Venus flytrap were analyzed and reproduced in the discrete electrical circuit. The information gained from this study can be used to elucidate the coupling of intracellular and intercellular communications in the form of electrical signals within plants. 相似文献
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