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排序方式: 共有557条查询结果,搜索用时 15 毫秒
61.
T D Volkova O M Vol'pina M N Zhmak V T Ivanov M F Vorovich A V Timofeev 《Bioorganicheskaia khimiia》2001,27(3):174-179
Six peptide fragments of the envelope protein E of the tick-borne encephalitis virus involving the predicted T-helper epitopes were synthesized. Their ability to induce antibodies without conjugation with any high-molecular-mass carrier was studied in mice of three lines. Five of six synthesized peptides exhibited immunogenic properties, which differed in dependence on the haplotype of immunized mice. The peptide binding to the antiviral antibodies was studied, and two peptides were revealed that demonstrated a high ability to recognize the viral antibodies in the horse and human sera. These peptides are promising for the development of diagnostic agents for the tick-borne encephalitis virus. 相似文献
62.
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64.
M. B. Oboznaya N. M. Vladimirova M. A. Titova T. D. Volkova D. O. Koroev A. A. Ryabokon’ A. A. Egorov S. S. Rybakov O. M. Vol’pina 《Russian Journal of Bioorganic Chemistry》2008,34(6):674-682
Antibodies to the prion protein (PrP), particularly, monoclonal antibodies, are necessary tools in the diagnostics and study of prion diseases and potential means of their immunotherapy. For the production of monoclonal antibodies, BALB/c mice were immunized by a recombinant bovine PrP. Three stable hybridomas producing antibodies of IgM class were prepared. The antibodies were bound to PrP in a solid-phase enzyme immunoassay and immunoblotting. The epitope mapping accomplished with the use of synthetic peptides showed that an epitope located in region 25–36 of PrP corresponds to one antibody, and epitopes located in region 222–229, to the other two. The antibodies to fragment 222–229 purified by affinity chromatography recognized with a high specificity conglomerates of a pathogenic prion in the brain tissue of cows suffering from spongiform encephalopathy. Thus, in nontransgenic mice, PrP-specific monoclonal antibodies were produced, useful in studies and diagnostics of prion diseases. 相似文献
65.
S. V. Bitarishvili P. Yu. Volkova S. A. Geras’kin 《Russian Journal of Plant Physiology》2018,65(3):446-454
We investigated the effect of γ-irradiation (4–50 Gy) of barley seeds (Hordeum vulgare L., cv. Nur) on the content of endogenous phytohormones–stimulators of plant growth and development: indol-3-acetic acid (IAA), indolyl-3-butyric acid (IBA), zeatin and abscisic acid (ABA). The ratio (IAA + IBA + zeatin)/ABA from the third to the seventh day of germination has been measured. It was shown that the changes in the content of phytohormones as a function of the radiation dose were nonlinear. In the dose range of 4–20 Gy, phytohormones balance was changed due to increased content of growth stimulators and decreased ABA content. Using a dose of 50 Gy led primarily to a decrease in the content of growth stimulators and an increase in ABA content, and the ratio (IAA + IBA + zeatin)/ABA shifted toward ABA content. 相似文献
66.
Entomological Review - A new genus, Wittinia gen. n., is proposed for Megalopygidae moths restricted to the Western Hemisphere. Two species are included: Wittinia cremor sp. n., the type species... 相似文献
67.
Selective Electroreduction of CO2 toward Ethylene on Nano Dendritic Copper Catalysts at High Current Density 下载免费PDF全文
Christian Reller Ralf Krause Elena Volkova Bernhard Schmid Sebastian Neubauer Andreas Rucki Manfred Schuster Günter Schmid 《Liver Transplantation》2017,7(12)
In situ deposited copper nanodendrites are herein proven to be a highly selective electrocatalyst which is capable of reducing CO2 to ethylene by reaching a Faradaic efficiency of 57% at a current density of 170 mA cm?2. It is found that the desired structures are formed in situ under acidic pH conditions at high electrode potentials more negative than ?2 V versus Ag/AgCl. Detailed investigations on the preparation, characterization, and advancement of electrode materials and of the electrolyte have been performed. Catalyst degradation effects are intensively followed by scanning electron microscopy (SEM) and high‐resolution transmission electron microscopy (HR‐TEM) characterization methods and found to be a major root course for selectivity losses. 相似文献
68.
The objective of the present investigation was to study the influence of size, nature, and topology of substituents on the
thermodynamic characteristics of sublimation, fusion, solubility, solvation, and partitioning processes of some drug and druglike
molecules. Thermodynamic functions of sublimation process 2-acetaminophen and 3-acetaminophen were obtained on the basis of
temperature dependencies of vapor pressure by the transpiration method. Thermodynamic characteristics of solubility processes
in water, n-octanol, and n-hexane were calculated from the temperature dependencies of solubility using the solubility saturation method. For evaluation
of fusion parameters, differential scanning calorimetry was used. A new approach to distinguishing specific and nonspecific
energetic terms in the crystal lattice was developed. Specific and nonspecific solvation terms were distinguished using the
transfer from the “inert” n-hexane to the other solvents. For the acetaminophen compounds and for some related drug molecules, the correlation between
melting points and a parameter describing the ratio between specific and nonspecific interaction in the crystal lattices was
obtained. A diagram enabling analysis of the mechanism of the partitioning process was applied. It was found that for isomers
of benzoic acids and for acetaminophens, the position of substituents affects the mechanism of the partitioning process but
not the extent of partitioning ( values). In contrast to this, an increased size of substituents (parabens) leads to essential changes in values, but the mechanism of the partitioning process stays the same. 相似文献
69.
Zholobov VE Dvorak SI Uliukin IM Vinogradova EN Volkova GV Tiusova OV 《Zhurnal mikrobiologii, epidemiologii, i immunobiologii》2008,(3):79-81
Dynamics of HIV-infection epidemic in St. Petersburg was analyzed. New features of epidemic process, which were not observed in St. Petersburg earlier, whereas noted in other territories of Russia, were revealed and described. 相似文献
70.
E. G. Volkova S. Y. Kurchashova E. V. Sheval V. Y. Polyakov 《Biochemistry (Moscow) Supplemental Series A: Membrane and Cell Biology》2009,3(4):404-409
The nuclear pore complexes are complex protein structures located in the nuclear envelope, where they control the nuclear-cytoplasmic
transport, and inside the stacks of endoplasmic reticulum cisternae, annulate lamellae. After overexpression of some nucleoporins,
numerous granules are visible in the cytoplasm. According to the published data, these granules are the annulate lamellae.
In the current paper, the structural organization of POM121-containing granules was analyzed using correlative light and electron
microscopy. The ultrastructural study demonstrates that POM121-containing granules are not annulate lamellae but aggregates
of endoplasmic reticulum membranes. Thus, overexpressed POM121 is not able to induce the annulate lamella formation. The mechanisms
of self-organization of non-functional structures (such as the aggregates of endoplasmic reticulum membranes described here)
and possible involvement of these mechanisms in the formation of cellular structures are discussed. 相似文献