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301.
Anna Akhmanova Frank G. J. Voncken Ken M. Hosea Harry Harhangi Jan T. Keltjens Huub J. M. op den Camp Godfried D. Vogels & Johannes H. P. Hackstein 《Molecular microbiology》1999,32(5):1103-1114
The chytrid fungi Piromyces sp. E2 and Neocallimastix sp. L2 are obligatory amitochondriate anaerobes that possess hydrogenosomes. Hydrogenosomes are highly specialized organelles engaged in anaerobic carbon metabolism; they generate molecular hydrogen and ATP. Here, we show for the first time that chytrid hydrogenosomes use pyruvate formate-lyase (PFL) and not pyruvate:ferredoxin oxidoreductase (PFO) for pyruvate catabolism, unlike all other hydrogenosomes studied to date. Chytrid PFLs are encoded by a multigene family and are abundantly expressed in Piromyces sp. E2 and Neocallimastix sp. L2. Western blotting after cellular fractionation, proteinase K protection assays and determinations of enzyme activities reveal that PFL is present in the hydrogenosomes of Piromyces sp. E2. The main route of the hydrogenosomal carbon metabolism involves PFL; the formation of equimolar amounts of formate and acetate by isolated hydrogenosomes excludes a significant contribution by PFO. Our data support the assumption that chytrid hydrogenosomes are unique and argue for a polyphyletic origin of these organelles. 相似文献
302.
Reversible activation of allantoate amidohydrolase by acid-pretreatment and other properties of the enzyme 总被引:5,自引:0,他引:5
G D Vogels 《Biochimica et biophysica acta》1966,113(2):277-291
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J T Keltjens H J Kraft W G Damen C van der Drift G D Vogels 《European journal of biochemistry》1989,184(2):395-403
The hydrogen-dependent reduction of methylcoenzyme M catalyzed by coenzyme-depleted cell-free extracts of Methanobacterium thermoautotrophicum was stimulated by micromolar concentrations of a UDP-disaccharide present in the organism. The compound was isolated and identified as UDP-1-O-alpha-D-2-acetamido-2-deoxyglucopyranose (UDPGlcpNAc) glycosidically linked to 2-acetamido-2-deoxymannopyranosyluronic acid. Maximal stimulation was observed when both the UDP-disaccharide and mercaptoheptanoylthreonine phosphate were present in the reaction mixtures. The UDP derivative isolated was not specific in its action: other UDP-sugars tested in micromolar concentrations stimulated the methylcoenzyme M reduction to the same extent. The activated sugars presumably substitute for ATP, which is usually required in much higher concentrations to activate the methylcoenzyme M reductase system. 相似文献
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306.
Improved identification of methanogenic bacteria by fluorescence microscopy. 总被引:21,自引:9,他引:12
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Methanogenic bacteria can be tentatively identified by fluorescence microscopy. This technique was improved by carefully selecting a series of excitation and barrier filters that matched the excitation and emission spectra of some unique coenzymes viz., F420 and F350, in methanogenic bacteria. 相似文献
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Cells ofPseudomonas aeruginosa were grown in a synthetic medium containing allantoin as the sole source of carbon, nitrogen and energy. The specific activity of allantoicase in the cell-free extract amounted to 7 units/mg protein. The enzyme was purified 190 times by treatment of the extract at 72 C in the presence of Mn2+ions and chromatography on Ecteola-cellulose. 相似文献
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