全文获取类型
收费全文 | 1710篇 |
免费 | 73篇 |
出版年
2022年 | 7篇 |
2021年 | 16篇 |
2020年 | 8篇 |
2019年 | 8篇 |
2018年 | 16篇 |
2017年 | 11篇 |
2016年 | 27篇 |
2015年 | 45篇 |
2014年 | 62篇 |
2013年 | 80篇 |
2012年 | 128篇 |
2011年 | 95篇 |
2010年 | 74篇 |
2009年 | 71篇 |
2008年 | 98篇 |
2007年 | 111篇 |
2006年 | 89篇 |
2005年 | 115篇 |
2004年 | 103篇 |
2003年 | 115篇 |
2002年 | 91篇 |
2001年 | 20篇 |
2000年 | 20篇 |
1999年 | 21篇 |
1998年 | 42篇 |
1997年 | 15篇 |
1996年 | 21篇 |
1995年 | 20篇 |
1994年 | 22篇 |
1993年 | 21篇 |
1992年 | 15篇 |
1991年 | 15篇 |
1990年 | 7篇 |
1989年 | 11篇 |
1988年 | 7篇 |
1986年 | 9篇 |
1985年 | 11篇 |
1984年 | 15篇 |
1983年 | 12篇 |
1982年 | 15篇 |
1981年 | 15篇 |
1980年 | 10篇 |
1979年 | 7篇 |
1978年 | 10篇 |
1977年 | 5篇 |
1976年 | 9篇 |
1975年 | 7篇 |
1974年 | 9篇 |
1970年 | 3篇 |
1966年 | 3篇 |
排序方式: 共有1783条查询结果,搜索用时 58 毫秒
881.
Degrelle SA Jaffrezic F Campion E Lê Cao KA Le Bourhis D Richard C Rodde N Fleurot R Everts RE Lecardonnel J Heyman Y Vignon X Yang X Tian XC Lewin HA Renard JP Hue I 《PloS one》2012,7(6):e38309
Somatic cell nuclear transfer (SCNT) is the most efficient cell reprogramming technique available, especially when working with bovine species. Although SCNT blastocysts performed equally well or better than controls in the weeks following embryo transfer at Day 7, elongation and gastrulation defects were observed prior to implantation. To understand the developmental implications of embryonic/extra-embryonic interactions, the morphological and molecular features of elongating and gastrulating tissues were analysed. At Day 18, 30 SCNT conceptuses were compared to 20 controls (AI and IVP: 10 conceptuses each); one-half of the SCNT conceptuses appeared normal while the other half showed signs of atypical elongation and gastrulation. SCNT was also associated with a high incidence of discordance in embryonic and extra-embryonic patterns, as evidenced by morphological and molecular "uncoupling". Elongation appeared to be secondarily affected; only 3 of 30 conceptuses had abnormally elongated shapes and there were very few differences in gene expression when they were compared to the controls. However, some of these differences could be linked to defects in microvilli formation or extracellular matrix composition and could thus impact extra-embryonic functions. In contrast to elongation, gastrulation stages included embryonic defects that likely affected the hypoblast, the epiblast, or the early stages of their differentiation. When taking into account SCNT conceptus somatic origin, i.e. the reprogramming efficiency of each bovine ear fibroblast (Low: 0029, Med: 7711, High: 5538), we found that embryonic abnormalities or severe embryonic/extra-embryonic uncoupling were more tightly correlated to embryo loss at implantation than were elongation defects. Alternatively, extra-embryonic differences between SCNT and control conceptuses at Day 18 were related to molecular plasticity (high efficiency/high plasticity) and subsequent pregnancy loss. Finally, because it alters re-differentiation processes in vivo, SCNT reprogramming highlights temporally and spatially restricted interactions among cells and tissues in a unique way. 相似文献
882.
883.
P Maiuri E Terriac P Paul-Gilloteaux T Vignaud K McNally J Onuffer K Thorn PA Nguyen N Georgoulia D Soong A Jayo N Beil J Beneke JC Hong Lim C Pei-Ying Sim YS Chu;WCR participants A Jiménez-Dalmaroni JF Joanny JP Thiery H Erfle M Parsons TJ Mitchison WA Lim AM Lennon-Duménil M Piel M Théry 《Current biology : CB》2012,22(17):R673-R675
Motility is a common property of animal cells. Cell motility is required for embryogenesis [1], tissue morphogenesis [2] and the immune response [3] but is also involved in disease processes, such as metastasis of cancer cells [4]. Analysis of cell migration in native tissue in vivo has yet to be fully explored, but motility can be relatively easily studied in vitro in isolated cells. Recent evidence suggests that cells plated in vitro on thin lines of adhesive proteins printed onto culture dishes can recapitulate many features of in vivo migration on collagen fibers [5,6]. However, even with controlled in vitro measurements, the characteristics of motility are diverse and are dependent on the cell type, origin and external cues. One objective of the first World Cell Race was to perform a large-scale comparison of motility across many different adherent cell types under standardized conditions. To achieve a diverse selection, we enlisted the help of many international laboratories, who submitted cells for analysis. The large-scale analysis, made feasible by this competition-oriented collaboration, demonstrated that higher cell speed correlates with the persistence of movement in the same direction irrespective of cell origin. 相似文献
884.
Fouchet D Verrier D Ngoubangoye B Souquière S Makuwa M Kazanji M Gonzalez JP Pontier D 《Proceedings. Biological sciences / The Royal Society》2012,279(1742):3426-3435
Understanding how pathogens spread and persist in the ecosystem is critical for deciphering the epidemiology of diseases of significance for global health and the fundamental mechanisms involved in the evolution of virulence and host resistance. Combining long-term behavioural and epidemiological data collected in a naturally infected mandrill population and a Bayesian framework, the present study investigated unknown aspects of the eco-epidemiology of simian immunodeficiency virus (SIV), the recent ancestor of HIV. Results show that, in contrast to what is expected from aggressive and sexual transmission (i.e. the two commonly accepted transmission modes for SIV), cases of SIVmnd-1 subtype were significantly correlated among related individuals (greater than 30% of the observed cases). Challenging the traditional view of SIV, this finding suggests the inheritance of genetic determinants of susceptibility to SIV and/or a role for behavioural interactions among maternal kin affecting the transmission of the virus, which would highlight the underappreciated role of sociality in the spread of infectious diseases. Outcomes of this study also provide novel insights into the role of host social structure in the evolution of pathogens. 相似文献
885.
886.
Cambier S Gonzalez P Mesmer-Dudons N Brèthes D Fujimura M Bourdineaud JP 《Biometals》2012,25(1):165-180
The neurotoxic compound methylmercury (MeHg) is a commonly encountered pollutant in the environment, and constitutes a hazard
for wildlife and human health through fish consumption. To study the neurotoxic impact of MeHg on piscivorous fish, we contaminated
the model fish species Danio rerio for 25 and 50 days with food containing 13.5 μg/g dry weight (dw) of MeHg (0.6 μg MeHg/fish/day), an environmentally relevant
dose leading to brain mercury concentrations of 30 ± 4 μg of Hg g−1 (dw) after 25 days of exposure and 46 ± 7 μg of Hg g−1 (dw) after 50 days. Brain mitochondrial respiration was not modified by exposure to MeHg, contrary to what happens in skeletal
muscles. A 6-fold increase in the expression of the sdh gene encoding the succinate dehydrogenase Fe/S protein subunit was detected in the contaminated brain after 50 days of exposure.
An up regulation of 3 genes, atp2b3a, atp2b3b, and slc8a2b, encoding for calcium transporters was noticed after 25 days of exposure but the atp2b3a and atp2b3b were repressed and the slc8a2b gene expression returned to its basal level after 50 days, suggesting a perturbation of calcium homeostasis. After 50 days,
we detected the up regulation of glial fibrillary acidic protein and glutathione S-transferase genes (gfap and gst), along with a repression of the glutathione peroxidase gene gpx1. These results match well with a MeHg-induced onset of oxidative stress and inflammation. A transmission electron microscopic
observation confirmed an impairment of the optical tectum integrity, with a decrease of the nucleal area in contaminated granular
cells compared to control cells, and a lower density of cells in the contaminated tissue. A potential functional significance
of such changes observed in optical tectum when considering wild fish contaminated in their natural habitat might be an impaired
vision and therefore a lowered adaptability to their environment. 相似文献
887.
888.
889.
890.
JP Maalouf Y Le Bagousse-Pinguet L Marchand B Touzard R Michalet 《Annals of botany》2012,110(4):821-828
Background and Aims There is still debate regarding the direction and strength of plant interactions under intermediate to high levels of stress. Furthermore, little is known on how disturbance may interact with physical stress in unproductive environments, although recent theory and models have shown that this interplay may induce a collapse of plant interactions and diversity. The few studies assessing such questions have considered the intensity of biotic interactions but not their importance, although this latter concept has been shown to be very useful for understanding the role of interactions in plant communities. The objective of this study was to assess the interplay between stress and disturbance for plant interactions in dry calcareous grasslands. Methods A field experiment was set up in the Dordogne, southern France, where the importance and intensity of biotic interactions undergone by four species were measured along a water stress gradient, and with and without mowing disturbance. Key Results The importance and intensity of interactions varied in a very similar way along treatments. Under undisturbed conditions, plant interactions switched from competition to neutral with increasing water stress for three of the four species, whereas the fourth species was not subject to any significant biotic interaction along the gradient. Responses to disturbance were more species-specific; for two species, competition disappeared with mowing in the wettest conditions, whereas for the two other species, competition switched to facilitation with mowing. Finally, there were no significant interactions for any species in the disturbed and driest conditions. Conclusions At very high levels of stress, plant performances become too weak to allow either competition or facilitation and disturbance may accelerate the collapse of interactions in dry conditions. The results suggest that the importance and direction of interactions are more likely to be positively related in stressful environments. 相似文献