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61.
The contribution deals with the phytocenological analysis of theRondeletio correifoliae-Andropogonetum multinervosi, an endemic savanna association occuring on the Siguanea Hills (Sierra de la Siguanea) near the Colony Hotel. 相似文献
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Lj. Vitale M. Renko B. Lenarčič V. Turk M. Pokorny 《Applied microbiology and biotechnology》1986,23(6):449-455
Summary A leucine aminopeptidase was purified to homogeneity fromStreptomyces rimosus culture filtrates, which are waste broth of oxytetracycline bioproduction process. Purification procedure includes ultrafiltration
and chromatography on CM-Sephadex, AH-Sepharose and FPLC Mono S column.
The enzyme is a monomer with molecular weight of 27,500 Daltons and pI of 7.3, stable in broad pH range and up to 70°C. It
is a metallo enzyme dependent on Ca2+ ions for its full activity. By its specificity it is a true aminopeptidase active on amino acid amide, arylamide, peptide
and ester bonds. The hydrolysing activity shows preference for leucine at the N-terminal position of substrates, also acts
on aromatic acids and methionine, but does not release glycine, proline, acidic amino acids orD-amino acid residues. 相似文献
64.
Lipopolysaccharide core mutants of Salmonella typhimurium containing D-glycero-D-manno-heptose 总被引:1,自引:0,他引:1
Mutants resistant to several hydrophobic membrane antagonists were isolated from a "deep rough" (rfaC) mutant of Salmonella typhimurium. The resistance was due to an alteration in the core region lipopolysaccharide composition as evidenced by altered bacteriophage and complement sensitivity and by compositional analysis. The principal change in carbohydrate composition was the predominance of the unusual heptose isomer D-glycero-D-manno-heptose. The unusually wide pleiotropic phenotype of this organism is suggested to be due to a fundamental change in the properties of the bacterial outer membrane. 相似文献
65.
Agrobacterium radiobacter, strain B6 (a strain isolated in this laboratory, which limited the occurrence of damping-off of sugar beet and influenced growth of plants in hot-house and field experiments) was found to produce an acidic exopolysaccharide in a mineral medium with various carbon sources. Hydrolyzates of the polysaccharide contained glucose, galactose, glycerol, succinic acid and pyruvic acid, whose quantitative content varied according to the carbon source used. The polysaccharide isolated from the medium containing glucose exhibited the highest physiological activity. Seeds germinated best and sugar beet roots were found to grow most rapidly in a medium containing 0.2 % (W/W) of the polysaccharide. The roots exposed for 3 d in this medium grew 2.7-fold as compared with non-treated plants. Higher sumbers of microorganisms were detected on the surface of roots treated with the polysaccharide. Growth of roots was also stimulated when immersing the seeds (30 min) in a 0.2 –0.4 % solution of this polysaccharide. After a two-fold treatment the roots were less damaged by the fungusPythium ultimum. Plants from seeds treated with the polysaccharide grew in the field soil more rapidly than the non-treated plants but worse than after bacterization of the seeds byA. radiobacter B6 and were only partially protected against the damping-off of sugar beet. 相似文献
66.
Oudin’s principle of single immunodiffusion in agar gel was modified for quantitative determination of IgA in bronchoalveolar lavage (BAL) of normal 20–25 g mice. The reaction took place at 25 °C in 0.3 % agarose with 16.7 % pig serum against mouse IgA, and was evaluated on the basis of a relationship between the progress of the precipitin zone and the square root of time. The linear dependence of the derived constantk on the logarithmic concentration of antibody in the sample permitted to express the results as titre, corresponding to a dilution wherek = 0. Examination of seven samples of pooled blood serum of normal mice shoved taht (1) the IgA level was practically constant, (2) serum IgA possessed under given conditions similar properties as IgA from the bronchoalveolar secretion; it is therefore possible to employ pooled sera as a reliable control of the immunodiffusion system in ease of lack of reference standards with defined IgA content. Examination of 82 individual BAL samples of normal mice revealed that the mean IgA concentration in 2.5 mL samples was almost 1000 times lower than in blood serum. 相似文献
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Vera Hadačová Květa Vacková Eva Klozová M. Kutáček Květa Pitterová 《Biologia Plantarum》1983,25(3):209-215
In partly purified protein complexes obtained from 22 species of theAllium genus and 6 cultivars ofAllium cepa the activity of cholinesterases was detected and measured using the method of Ellman et al. The degree of its inhibition with 10-4 M neostigmine was also tested. It was found that the activity of cholinesterase differed in individual species up to two hundred times, while the differences in the inhibitory activity of 10-4 M neostigmine occurred only in a few cases. Individual sections and cultivars could not be characterized on the basis of the differences in the activities of the cholinesterases. Of all the sections that ofPhyllodolon shows the highest average activity. In the case of the tested cultivars distinctly the lowest activity was observed in cv. Kastická. The values of the enzymatic activity measured by Ellman’s method in this plant material include the activity of specific and unspecific cholinesterases and the part uninhibitable by neostigmine. 相似文献
70.
Eva Klozová Jiřina Švachulová J. Smartt E. Hadač Věra Turková Véra Hadačová 《Biologia Plantarum》1983,25(4):266-273
The seed protein patterns of 12Arachis species were compared by polyacrylamide gel electrophoresis (PAGE), similarities between patterns were measured by the Jaccard
index. Results obtained confirm the close relationships established between members of the genus on morphological grounds
and support the more recent classification schemes.A. villosa andA. correntina could well be regarded as distinct species on grounds of protein differences whileA. macedoi andA. villosulicarpa (although members of the same section, Extranervosae) show considerable differentiation of their protein patterns. Surprisingly,
the formA. ×batizogaea showed less similarity in protein pattern to those of its parental species than might have been expected. The principle value
of seed protein pattern data appears to be in distinguishing species within sections. 相似文献