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81.
82.
Laktionov P Chelobanov B Rykova E Vlassov V 《Nucleosides, nucleotides & nucleic acids》2001,20(4-7):859-862
Oligonucleotides (ODNs) conjugated to 4-[(N-2-chloroethyl-N-methyl)amino] benzylamine were used to investigate ODN-binding proteins in cells of different origin. The data obtained demonstrate that 68, 46, 38 and 28 kDa ODN-binding proteins are universal for tested cell lines. 相似文献
83.
The interaction of antisense oligodeoxyribonucleotides with yeast tRNA(Phe) was investigated. 14-15-mers complementary to the 3'-terminal sequence including the ACCA end bind to the tRNA under physiological conditions. At low oligonucleotide concentrations the binding occurs at the unique complementary site. At higher oligonucleotide concentrations, the second oligonucleotide molecule binds to the complex due to non-perfect duplex formation in the T-loop stabilized by stacking between the two bound oligonucleotides. In these complexes the acceptor stem is open and the 5'-terminal sequence of the tRNA is accessible for binding of a complementary oligonucleotide. The results prove that the efficient binding of oligonucleotides to the 3'-terminal sequence of the tRNA occurs through initial binding to the single-stranded sequence ACCA followed by invasion in the acceptor stem and strand displacement. 相似文献
84.
Koval'ov N Kuznetsova I Burakova E Sil'nikov V Zenkova M Vlassov V 《Nucleosides, nucleotides & nucleic acids》2004,23(6-7):977-981
Cationic compounds containing benzene ring substituted with the bis-quaternary salt of diazabicyclo[2.2.2]octane (DABCO) bearing a polymethylene fragment at the bridge positions display ribonuclease activity. Efficacy of the catalysis is affected by geometry of the cationic structures and the size of the attached aliphatic fragment. The cleavage occurs primarily within CA sequences. The compounds do not possess tradition groups participating in the transesterification step of RNA cleavage reaction, therefore a speculative mechanism of cleavage could be inducing a conformational stress on the RNA sugar phosphate backbone providing fragility to phosphodiester bonds. 相似文献
85.
Silencing of c-myc expression in tumor cells by siRNA 总被引:1,自引:0,他引:1
Kabilova TO Chernolovskaya EL Vladimirova AV Vlassov VV 《Nucleosides, nucleotides & nucleic acids》2004,23(6-7):867-872
86.
Laktionov PP Chelobanov BP Kharkova MV Rykova EY Pyshnyi DV Pyshnaya IA Marcus K Meyer HE Vlassov VV 《Nucleosides, nucleotides & nucleic acids》2003,22(5-8):1715-1719
Affinity modified with Flu-DAP-p(N)16degU oligonucleotide-binding proteins were isolated by affinity chromatography using Ultrogel A2-anti fluorescein antibodies. After separation by SDS-PAGE the proteins with molecular masses about 68 kDa were MS/MS sequenced and identified as keratin K1, keratin K10, keratin K2e and albumin. 相似文献
87.
A. V. Cherepanova S. N. Tamkovich V. V. Vlassov P. P. Laktionov 《Biochemistry (Moscow) Supplemental Series B: Biomedical Chemistry》2007,1(4):299-304
The review summarizes literature data on (deoxy)ribonuclease activity in blood of healthy donors and patients with diseases. Special attention is paid to methodological aspects of the analysis of deoxyribonuclease activity and factors, which interfere with enzyme activity in blood. 相似文献
88.
We report on the formation of novel RNA molecules in a recombination-like, nonenzymatic reaction proceeding in the complex of partially complementary RNA-oligonucleotides under very simple conditions. Analysis of the isolated products demonstrated that at least 5% of the formed linkages are of the (natural) 3',5'-phosphodiester type. We suggest that similar reactions could contribute to the development of the 'RNA world', but could also proceed in vivo within variously structured RNA or RNA complexes containing loops, bulges, or dangling ends, providing an emergence of novel RNA sequences. 相似文献
89.
Miroshnichenko S. K. Amirloo B. Bichenkova E. V. Vlassov V. V. Zenkova M. A. Patutina O. A. 《Russian Journal of Bioorganic Chemistry》2019,45(6):803-812
Russian Journal of Bioorganic Chemistry - Nowadays, application of miRNases—artificial ribonucleases aimed at degradation of noncoding RNAs, in particular, miRNAs—represents one of the... 相似文献
90.
T. V. Abramova J. P. Leonetti V. V. Vlassov B. Lebleu 《Russian Journal of Bioorganic Chemistry》2000,26(3):174-182
Dinucleoside phosphates that harbor phosphate groups transiently blocked (caged) byo-nitrobenzyl oro-nitroveratryl residues were synthesized. It was shown that the conditions of the UV-induced deprotection largely depend on
the nature of the protective group. The phosphotriesters obtained were resistant toward snake venom phosphodiesterase and
nucleases of the cellular extract. The synthesis of the dinucleoside phosphates containing a photolabile group preceeded the
incorporation of the modified blocks into extended oligonucleotides by the phosphoramidite method. 相似文献