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排序方式: 共有302条查询结果,搜索用时 15 毫秒
31.
Mohammed Mamdani Vernell Williamson Gowon O. McMichael Tana Blevins Fazil Aliev Amy Adkins Laura Hack Tim Bigdeli Andrew D. van der Vaart Bradley Todd Web Silviu-Alin Bacanu Gursharan Kalsi COGA Consortium Kenneth S. Kendler Michael F. Miles Danielle Dick Brien P. Riley Catherine Dumur Vladimir I. Vladimirov 《PloS one》2015,10(9)
32.
The functional heterogeneity of circulating polymorphonuclear leukocytes in acute myocardial infarct
M V Kre?nina G I Klebanov S V Kuznetsov R M Vakoliuk Iu A Vladimirov 《Biulleten' eksperimental'no? biologii i meditsiny》1989,108(12):673-676
Cytofluorometric analysis, using immune complexes, consisting of human IgG and fluorescein isothiocianate (FITC)-labelled monospecific rabbit antibodies to human IgG, as fluorescent markers for polymorphonuclear leukocytes (PML), and microscopic analysis of modified NST-test probes with NADH2 or NADFH2 were employed to investigate PML functional heterogeneity of healthy individuals and patients with acute myocardial infarction. It was shown, that circular PML make up heterogeneous cell population in the following parameters: Fc gamma-receptors expression, oxidative derivates generation and cell oxidases activity and the degree of heterogeneity may change during nonspecific inflammatory process, which accompanies acute myocardial infarction. 相似文献
33.
Plants of the genus Linaria are scientifically interesting owing to the presence of natural-transgenic forms containing a set of agrobacterial genes (so-called cT-DNA). Many Linaria species are valuable ornamental plants. However, until recently, the literature did not describe the method of genetic transformation of plants of the genus Linaria. In this paper, we present the method of in vitro agrobacterial transformation of plants of Moroccan toadflax (Linaria maroccana Hook. f.). Method of adaptation for in vivo conditions is also described. This technique will be used in further studies of the functioning of сT-DNA and can also be recommended for solving applied problems. 相似文献
34.
E. V. Navolotskaya V. B. Sadovnikov D. V. Zinchenko V. I. Vladimirov Y. A. Zolotarev 《Russian Journal of Bioorganic Chemistry》2018,44(4):403-407
We prepared 125I-labeled cholera toxin B subunit (125I-labeled CT-B, specific activity 98 Ci/mmol) and found that its binding to rat IEC-6 intestinal epithelial cells was high-affinity (Kd 1.9 nM). The binding of labeled protein was completely inhibited by unlabeled thymosin-α1 (TM-α1), interferon-α2 (IFN-α2), and synthetic peptide LKEKK, which corresponds to residues 16–20 in TM-α1 and 131–135 in IFN-α2 (Ki 1.2, 0.9, and 1.6 nM, respectively), but was not inhibited by synthetic peptide KKEKL with inverted amino acid sequence (Ki > 10 μM). Thus, TM-α1, IFN-α2, and the LKEKK peptide bind with high affinity and specificity to CT-B receptor on rIEC-6 cells. It was found that CT-B and the LKEKK peptide at concentrations of 10–1000 nM increased nitric oxide production and soluble guanylate cyclase activity in the cells in a dose-dependent manner. 相似文献
35.
Kapralov AA Kurnikov IV Vlasova II Belikova NA Tyurin VA Basova LV Zhao Q Tyurina YY Jiang J Bayir H Vladimirov YA Kagan VE 《Biochemistry》2007,46(49):14232-14244
Activation of peroxidase catalytic function of cytochrome c (cyt c) by anionic lipids is associated with destabilization of its tertiary structure. We studied effects of several anionic phospholipids on the protein structure by monitoring (1) Trp59 fluorescence, (2) Fe-S(Met80) absorbance at 695 nm, and (3) EPR of heme nitrosylation. Peroxidase activity was probed using several substrates and protein-derived radicals. Peroxidase activation of cyt c did not require complete protein unfolding or breakage of the Fe-S(Met80) bond. The activation energy of cyt c peroxidase changed in parallel with stability energies of structural regions of the protein probed spectroscopically. Cardiolipin (CL) and phosphatidic acid (PA) were most effective in inducing cyt c peroxidase activity. Phosphatidylserine (PS) and phosphatidylinositol bisphosphate (PIP2) displayed a significant but much weaker capacity to destabilize the protein and induce peroxidase activity. Phosphatidylinositol trisphosphate (PIP3) appeared to be a stronger inducer of cyt c structural changes than PIP2, indicating a role for the negatively charged extra phosphate group. Comparison of cyt c-deficient HeLa cells and mouse embryonic cells with those expressing a full complement of cyt c demonstrated the involvement of cyt c peroxidase activity in selective catalysis of peroxidation of CL, PS, and PI, which corresponded to the potency of these lipids in inducing cyt c's structural destabilization. 相似文献
36.
We have demonstrated that hypochlorite (HOCI/OCl-) and hypobromite (HOBr/OBr-) can react with tert-butyl hydroperoxide with close rate constants (k(HOCl) = 10,8 M(-1) x s(1); k(HOBr) = 8,9 M(-1) x (s(-1)). By means of the spin trap 4-pyridyl-1-oxide-N-tert-butyl nitron we have found that both reactions proceed through decomposition of tert-butyl hydroperoxide and generation of tert-butyl peroxyl (OOC(CH3)3) and tert-butoxyl (OC(CH3)3) radicals, the ratio of their the concentrations being dependent on the concentration of tert-butyl hydroperoxide. Thus, hypobromite, similar to hypochlorite, is a precursor of free radicals produced in the reaction with organic hydroperoxides. This reaction can be of great importance in the intensification of free radical processes, namely, in lipid peroxidation at the stage of chain branching. 相似文献
37.
M. V. Bilenko Yu. A. Vladimirov S. A. Pavlova Nguyen Thi Thu Thuy Tran Thi Hai Yen 《Biochemistry (Moscow) Supplemental Series B: Biomedical Chemistry》2009,3(1):64-70
Production of reactive oxygen species (ROS) by macrophages derived from blood monocytes of healthy donors (MPN) and patients with ischemic heart disease (IHD) (MPIHD) before, during, and after their incubation with low-density lipoprotein (LDL) isolated from blood plasma of healthy donors (LDLN) and patients with a high cholesterol level (LDLH) was investigated by the method of luminol-dependent (spontaneous) and stimulated chemiluminescence (CL) using opsonized zymosan (OZ) or phorbol-12-myristate-13-acetate (PMA) as the CL stimulators. It was shown that proper, luminol-dependent, and zymosan-or PMA-stimulated chemiluminescence of MPIHD was 1.4-, 1.8-, 2.7-, and 1.6-fold higher than the same types of chemiluminescence of MPN, respectively, (p<0.05–0.01). Although the effect of OZ on MPN and MPIHD was more potent than that of PMA (by 4.3- and 3.2-fold, respectively), but it appeared in 2.5–3.0 times slower than that of PMA. LDLN and LDLH incubated with MPN for the first 15 and 60 min caused the 1.4- and 2.5-increase of the luminol-dependent CL of MPN; the same treatment of MPIHD did not influence ROS production by these cells. Repeated increase in the OZ-stimulated CL of MPN was also observed after preincubation for 15–180 min with LDLN and LDLH followed by LDL removal, subsequent MPN washing and addition of Hanks solution and OZ; the repeated increase in OZ-stimulated CL of MPN was only observed after incubation with LDLH than with LDLN. No increase of CL was observed in experiments with MPIHD. Thus, more intensive chemiluminescence of macrophages obtained from blood of patients with IHD suggests their in vivo stimulation. LDLN and LDLH may cause both primary and secondary (after preincubation) stimulating effect on CL of MPN but not of MPIHD. Thus, the analysis of macrophage chemiluminescence is a sensitive test for evaluation the degree of macrophage stimulation; it may be effectively used for monitoring of effectiveness of medical treatment of patients. 相似文献
38.
N. R. Kildeeva P. A. Perminov L. V. Vladimirov V. V. Novikov S. N. Mikhailov 《Russian Journal of Bioorganic Chemistry》2009,35(3):360-369
The regularities of the reaction of aminopolysaccharide chitosan with glutaraldehyde (GA) have been considered. The equilibrium forms of GA in water have been thoroughly studied by NMR spectroscopy. It has been established that at pH 5.6, the exchange of the protons of O=CHCH2 groups for deuterium occurs, indicating the presence of an anion, a product of the first stage of the aldol reaction; at pH > 7.2, the formation of the products of an aldol reaction and aldol condensation takes place. The kinetics of the reaction between the amino groups of chitosan and GA, the kinetics of gel formation in chitosan solutions in the presence of GA, and the kinetics of changes in the rigidity of gels formed have been studied by UV spectroscopy. IR spectra of cross-linked chitosan have been obtained. It has been shown that chitosan catalyzes the polymerization of GA to form irregular products; in this process, the length of oligomeric chains in modified or cross-linked chitosan and the concentration of conjugated bonds increase with the GA concentration and pH of the reaction medium. 相似文献
39.
Yu. A. Vladimirov E. V. Proskurnina E. M. Demin N. S. Matveeva O. B. Lubitskiy A. A. Novikov D. Yu. Izmailov A. N. Osipov V. P. Tikhonov V. E. Kagan 《Biochemistry. Biokhimii?a》2009,74(3):301-307
Formation of free radicals in mitochondria plays a key role in the development of apoptosis, which includes formation of superoxide by the respiratory chain, formation of radicals by cytochrome c-cardiolipin complex in the presence of hydrogen peroxide or lipids, and chain lipid peroxidation resulting in cytochrome c release from mitochondria and initiation of the apoptotic cascade. In this work the effect of taxifolin (dihydroquercetin) and some other antioxidants on these three radical-producing reactions was studied. Peroxidase activity of the complex of cytochrome c with dioleyl cardiolipin estimated by chemiluminescence with luminol decreased by 50% with quercetin, taxifolin, rutin, Trolox, and ionol at concentrations 0.7, 0.7, 0.8, 3, and 10 μM, respectively. The lipid radical production detected by coumarin C-525-activated chemiluminescence decreased under the action of rutin and taxifolin in a dose-dependent manner, so that a 50% inhibition of chemiluminescence was observed at the antioxidant concentrations of 3.7 and 10 μM, respectively. Thus, these two radical-producing reactions responsible for apoptosis onset are inhibited by antioxidants at rather low concentrations. Experiments performed on liver slices and mash showed that taxifolin, quercetin, naringenin, and Trolox have low inhibitory effect on the lucigenin-dependent chemiluminescence in the tissue only at concentrations higher than 100 μM. 相似文献
40.
The review gives concepts of analysis of kinetics of complex reactions with the participation of free-radicals. The concepts are based on the comparison of the kinetic curves of the chemiluminescent reactions in the presence of a physical enhancer coumarin C-525 with the simulated kinetic functions. This method was applied to the investigation of the mechanism of a branched-chain reaction of lipid peroxidation in biological and phospholipid membranes as well as to the effect of antioxidants and determination of their activities. It was also used for studying the reaction of the formation of free radicals in the complex of cytochrome c with cardiolipin. This reaction plays a key role in the initiation of apoptosis. 相似文献