全文获取类型
收费全文 | 864篇 |
免费 | 119篇 |
出版年
2019年 | 10篇 |
2016年 | 14篇 |
2015年 | 13篇 |
2014年 | 20篇 |
2013年 | 27篇 |
2012年 | 49篇 |
2011年 | 39篇 |
2010年 | 17篇 |
2009年 | 25篇 |
2008年 | 28篇 |
2007年 | 58篇 |
2006年 | 35篇 |
2005年 | 24篇 |
2004年 | 30篇 |
2003年 | 32篇 |
2002年 | 36篇 |
2001年 | 24篇 |
2000年 | 20篇 |
1999年 | 23篇 |
1998年 | 11篇 |
1995年 | 10篇 |
1994年 | 15篇 |
1993年 | 10篇 |
1992年 | 13篇 |
1991年 | 20篇 |
1990年 | 15篇 |
1989年 | 20篇 |
1988年 | 14篇 |
1987年 | 15篇 |
1986年 | 12篇 |
1985年 | 17篇 |
1984年 | 22篇 |
1983年 | 9篇 |
1982年 | 10篇 |
1981年 | 19篇 |
1980年 | 12篇 |
1979年 | 16篇 |
1978年 | 11篇 |
1977年 | 9篇 |
1976年 | 8篇 |
1975年 | 11篇 |
1974年 | 15篇 |
1973年 | 11篇 |
1972年 | 15篇 |
1971年 | 6篇 |
1970年 | 12篇 |
1969年 | 8篇 |
1968年 | 10篇 |
1967年 | 6篇 |
1966年 | 10篇 |
排序方式: 共有983条查询结果,搜索用时 375 毫秒
81.
Watson KG Cameron R Fenton RJ Gower D Hamilton S Jin B Krippner GY Luttick A McConnell D MacDonald SJ Mason AM Nguyen V Tucker SP Wu WY 《Bioorganic & medicinal chemistry letters》2004,14(6):1589-1592
A set of trimeric and tetrameric derivatives 6-11 of the influenza virus neuraminidase inhibitor zanamivir 1 have been synthesized by coupling a common monomeric zanamivir derivative 3 onto various multimeric carboxylic acid core groups. These discrete multimeric compounds are all significantly more antiviral than zanamivir and also show outstanding long-lasting protective activity when tested in mouse influenza infectivity experiments. 相似文献
82.
83.
Ohren JF Chen H Pavlovsky A Whitehead C Zhang E Kuffa P Yan C McConnell P Spessard C Banotai C Mueller WT Delaney A Omer C Sebolt-Leopold J Dudley DT Leung IK Flamme C Warmus J Kaufman M Barrett S Tecle H Hasemann CA 《Nature structural & molecular biology》2004,11(12):1192-1197
MEK1 and MEK2 are closely related, dual-specificity tyrosine/threonine protein kinases found in the Ras/Raf/MEK/ERK mitogen-activated protein kinase (MAPK) signaling pathway. Approximately 30% of all human cancers have a constitutively activated MAPK pathway, and constitutive activation of MEK1 results in cellular transformation. Here we present the X-ray structures of human MEK1 and MEK2, each determined as a ternary complex with MgATP and an inhibitor to a resolution of 2.4 A and 3.2 A, respectively. The structures reveal that MEK1 and MEK2 each have a unique inhibitor-binding pocket adjacent to the MgATP-binding site. The presence of the potent inhibitor induces several conformational changes in the unphosphorylated MEK1 and MEK2 enzymes that lock them into a closed but catalytically inactive species. Thus, the structures reported here reveal a novel, noncompetitive mechanism for protein kinase inhibition. 相似文献
84.
Follicular dendritic cell dedifferentiation by treatment with an inhibitor of the lymphotoxin pathway dramatically reduces scrapie susceptibility 总被引:10,自引:0,他引:10 下载免费PDF全文
Transmissible spongiform encephalopathies (TSEs) may be acquired peripherally, in which case infectivity usually accumulates in lymphoid tissues before dissemination to the nervous system. Studies of mouse scrapie models have shown that mature follicular dendritic cells (FDCs), expressing the host prion protein (PrP(c)), are critical for replication of infection in lymphoid tissues and subsequent neuroinvasion. Since FDCs require lymphotoxin signals from B lymphocytes to maintain their differentiated state, blockade of this stimulation with a lymphotoxin beta receptor-immunoglobulin fusion protein (LT beta R-Ig) leads to their temporary dedifferentiation. Here, a single treatment with LT beta R-Ig before intraperitoneal scrapie inoculation blocked the early accumulation of infectivity and disease-specific PrP (PrP(Sc)) within the spleen and substantially reduced disease susceptibility. These effects coincided with an absence of FDCs in the spleen for ca. 28 days after treatment. Although the period of FDC dedifferentiation was extended to at least 49 days by consecutive LT beta R-Ig treatments, this had little added protective benefit after injection with a moderate dose of scrapie. We also demonstrate that mature FDCs are critical for the transmission of scrapie from the gastrointestinal tract. Treatment with LT beta R-Ig before oral scrapie inoculation blocked PrP(Sc) accumulation in Peyer's patches and mesenteric lymph nodes and prevented neuroinvasion. However, treatment 14 days after oral inoculation did not affect survival time or susceptibility, suggesting that infectivity may have already spread to the peripheral nervous system. Although manipulation of FDCs may offer a potential approach for early intervention in peripherally acquired TSEs, these data suggest that the duration of the treatment window may vary widely depending on the route of exposure. 相似文献
85.
A tumor-associated beta 1 integrin mutation that abrogates epithelial differentiation control 总被引:3,自引:0,他引:3 下载免费PDF全文
Evans RD Perkins VC Henry A Stephens PE Robinson MK Watt FM 《The Journal of cell biology》2003,160(4):589-596
SCC4 human keratinocytes are derived from a squamous cell carcinoma of the tongue and undergo very little spontaneous differentiation. Introduction of a wild-type beta 1 integrin subunit into SCC4 cells stimulates differentiation, suggesting either that the cells have a defect in the integrin signaling pathways that control differentiation or that the beta1 subunit itself is defective. Here we describe a heterozygous mutation in the SCC4 beta 1 subunit. The mutation, T188I, maps to the I-like domain. It results in constitutive activation of ligand binding, irrespective of the partner alpha subunit, in solid phase assays with recombinant protein and in living cells. The mutation promotes cell spreading, but not proliferation, motility, or invasiveness. It results in sustained activation of Erk MAPK independent of cell spreading. When introduced into SCC4 keratinocytes, the wild-type beta1 integrin stimulates differentiation, whereas the mutant is inactive. Activation of beta 1 integrins in normal keratinocytes also suppresses differentiation. These results establish, for the first time, mutation as a mechanism by which integrins can contribute to neoplasia, because the degree of differentiation in epithelial cancers is inversely correlated with prognosis. They also provide new insights into how integrins regulate keratinocyte differentiation. 相似文献
86.
87.
The effects of the Fanconi anemia zinc finger (FAZF) on cell cycle, apoptosis, and proliferation are differentiation stage-specific 总被引:5,自引:0,他引:5
Dai MS Chevallier N Stone S Heinrich MC McConnell M Reuter T Broxmeyer HE Licht JD Lu L Hoatlin ME 《The Journal of biological chemistry》2002,277(29):26327-26334
88.
FGFR1 is required for the development of the auditory sensory epithelium 总被引:12,自引:0,他引:12
The mammalian auditory sensory epithelium, the organ of Corti, comprises the hair cells and supporting cells that are pivotal for hearing function. The origin and development of their precursors are poorly understood. Here we show that loss-of-function mutations in mouse fibroblast growth factor receptor 1 (Fgfr1) cause a dose-dependent disruption of the organ of Corti. Full inactivation of Fgfr1 in the inner ear epithelium by Foxg1-Cre-mediated deletion leads to an 85% reduction in the number of auditory hair cells. The primary cause appears to be reduced precursor cell proliferation in the early cochlear duct. Thus, during development, FGFR1 is required for the generation of the precursor pool, which gives rise to the auditory sensory epithelium. Our data also suggest that FGFR1 might have a distinct later role in intercellular signaling within the differentiating auditory sensory epithelium. 相似文献
89.
Overcoming the constraints of long range radio telemetry from animals: getting more useful data from smaller packages 总被引:2,自引:1,他引:1
Many species carry out their most interesting activities wherethey cannot readily be observed or monitored. Marine mammalsare extreme among this group, accomplishing their most astoundingactivities both distant from land and deep in the sea. Collection,storage and transmission of data about these activities areconstrained by the energy requirements and size of the recordingloggers and transmitters. The more bits of information collected,stored and transmitted, the more battery is required and thelarger the tag must be. We therefore need to be selective aboutthe information we collect, while maintaining detail and fidelity.To accomplish this in the study of marine mammals, we have designed"intelligent" data logger/transmitters that provide context-drivendata compression, data relay, and automated data base storage.We later combine these data with remotely sensed environmentalinformation and other oceanographic data sets to recreate theenvironmental context for the animal's activity, and we displaythe combined data using computer animation techniques. In thisway, the system can provide near real time "observation" ofanimal behavior and physiology from the remotest parts of theglobe. 相似文献
90.
In metazoans, splicing of introns from pre-mRNAs can occur by two pathways: the major U2-dependent or the minor U12-dependent pathways. Whereas the U2-dependent pathway has been well characterized, much about the U12-dependent pathway remains to be discovered. Most of the information regarding U12-type introns has come from in vitro studies of a very few known introns of this class. To expand our understanding of U12-type splicing, especially to test the hypothesis that the simple base-pairing mechanism between the intron and U12 snRNA defines the branchpoint of U12-dependent introns, additional in vitro splicing substrates were created from three putative U12-type introns: the third intron of the Xenopus RPL1 a gene (XRP), the sixth intron of the Xenopus TFIIS.oA gene (XTF), and the first intron of the human Sm E gene (SME). In vitro splicing in HeLa nuclear extract confirmed U12-dependent splicing of each of these introns. Surprisingly, branchpoint mapping of the XRP splicing intermediate shows use of the upstream rather than the downstream of two consecutive adenosines within the branchpoint sequence (BPS), contrary to the prediction based on alignment with the sixth intron of human P120, a U12-dependent intron whose branch site was previously determined. Also, in the SME intron, the position of the branchpoint A residue within the region base paired with U12 differs from that in P120 and XTF. Analysis of these three additional introns therefore rules out simple models for branchpoint selection by the U12-type spliceosome. 相似文献