全文获取类型
收费全文 | 5444篇 |
免费 | 339篇 |
国内免费 | 1篇 |
专业分类
5784篇 |
出版年
2024年 | 8篇 |
2023年 | 51篇 |
2022年 | 93篇 |
2021年 | 187篇 |
2020年 | 134篇 |
2019年 | 158篇 |
2018年 | 208篇 |
2017年 | 199篇 |
2016年 | 234篇 |
2015年 | 325篇 |
2014年 | 373篇 |
2013年 | 420篇 |
2012年 | 425篇 |
2011年 | 408篇 |
2010年 | 251篇 |
2009年 | 205篇 |
2008年 | 286篇 |
2007年 | 246篇 |
2006年 | 252篇 |
2005年 | 192篇 |
2004年 | 173篇 |
2003年 | 155篇 |
2002年 | 145篇 |
2001年 | 99篇 |
2000年 | 55篇 |
1999年 | 67篇 |
1998年 | 21篇 |
1997年 | 21篇 |
1996年 | 32篇 |
1995年 | 17篇 |
1994年 | 21篇 |
1993年 | 14篇 |
1992年 | 29篇 |
1991年 | 27篇 |
1990年 | 21篇 |
1989年 | 16篇 |
1988年 | 19篇 |
1987年 | 22篇 |
1986年 | 20篇 |
1985年 | 23篇 |
1984年 | 17篇 |
1983年 | 10篇 |
1982年 | 11篇 |
1981年 | 8篇 |
1980年 | 10篇 |
1979年 | 11篇 |
1976年 | 6篇 |
1975年 | 6篇 |
1968年 | 9篇 |
1966年 | 5篇 |
排序方式: 共有5784条查询结果,搜索用时 0 毫秒
11.
Antonio Campos-Neto Fernanda W. M. Lima Arnaldo F. B. Andrade 《The Journal of eukaryotic microbiology》1985,32(1):84-88
A rapid and simple method for the purification of amastigotes of Trypanosoma cruzi from spleens of infected mice is described. A protein A-Scpharose 4B immunoadsorbent column bound with antisera to epimastigotes of T. cruzi was used to purify the tissue forms of this parasite. Host cells and debris are not retained, and parasites can be eluted in high yields and purity. Studies of surface glycoproteins and glycolipids of the purified amastigotes with 18 lectins of various specificities revealed the presence on the parasites of receptors for N-acetylglucosamine, N-acetylgalactosamine, D-galactose, and D-mannose binding lectins. 相似文献
12.
13.
Summary The effect of Triton X-100 on the activities of acid phosphatases from wheat germ, potato and human prostate was tested using -glycerophosphate, p-nitro-phenyl phosphate and naphthol AS BI phosphate as substrates. There was little effect on -glycerophosphatase activity at the concentrations of Triton X-100 tested. However at low concen trations of the detergent there was a stimulation of the activities of p-nitrophenyl phosphatase and naphthol phosphatase which were inhibited with the higher concentrations. Triton X-100 was found to enhance colour production between naphthol AS BI and fast red violet LB.Further evidence is presented confirming the presence of more than one acid phosphatase from each of the sources employed. 相似文献
14.
Shlomo Nir Nejat Düzgünes Maria C. Pedroso De Lima Dick Hoekstra 《Cell biochemistry and biophysics》1990,17(2):181-201
The fusion of viruses with cells and liposomes is reviewed with focus on the analysis of the final extents and kinetics of
fusion.Influenza virus andSendai virus exhibit 100% of fusion capacity with cells at pH 5 and pH 7.5, respectively. On the other hand, there may be in certain
cases, a limit on the number of virions that can fuse with a single cell, that is significantly below the limit on binding.
It still remains to be resolved whether this limit reflects a limited number of possible fusion sites, or a saturation limit
on the amount of viral glycoproteins that can be incorporated in the cellular membrane, like the case of virus fusion with
pure phospholipid vesicles, in which the fusion products were shown to consist of a single virus and several liposomes. Both
viruses demonstrate incomplete fusion activity towards liposomes of a variety of compositions. In the case ofSendai virus, fusion inactive virions bind essentially irreversibly to liposomes. Yet, preliminary results revealed that such bound,
unfused virions can be released by sucrose gradient centrifugation. The separated unfused virions subsequently fuse when incubated
with a “fresh” batch of liposomes. We conclude, therefore, that the fraction of initially bound unfused virions does not consist
of dective particles, but rather of particles bound to liposomes via “inactive” sites.
Details of the low pH inactivation of fusion capacity ofinfluenza virus towards cells and liposomes are presented. This inactivation is caused by protonation and exposure of the hydrophobic
segment of HA2, and affects primarily the fusion rate constants. Some degree of inactivation also occurs when virions are bound to cellular
membranes. 相似文献
15.
Alain Delbarre Philippe Muller Viviane Imhoff Jean-Louis Morgat Hélène Barbier-Brygoo 《Planta》1994,195(2):159-167
Uptake and metabolism of exogenous naphthalene-1-acetic acid (NAA) and indole-3-acetic acid (IAA) have been studied in tobacco (Nicotiana tabacum L. cv. Xanthi) mesophyll protoplasts. Both auxins entered protoplasts by diffusion under the action of the transmembrane pH gradient without any detectable participation of an influx carrier. Molecules were accumulated by an anion-trapping mechanism and most of them were metabolized within hours, essentially as glucose-ester and amino-acid conjugates. Protoplasts were equipped with a functional auxin-efflux carrier as evidenced by the inhibitory effect of naphthylphtalamic acid on IAA efflux. Basically, similar mechanisms of NAA and IAA uptake occurred in protoplasts. However, the two auxins differed in their levels of accumulation, due to different membrane-transport characteristics, and the nature of the metabolites produced. This shows the need to estimate the accumulation and the metabolism of auxins when analyzing their effects in a given cell system. The internal auxin concentration could be modulated by changing the transmembrane pH gradient, giving an interesting perspective for discriminating between the effects of intra- and extracellular auxin on physiological processes.Abbreviations BA
benzoic acid
- Ci/Ce
accumulation ratio of auxin
- IAAasp
N-[3-indolylacetyl]-dl-aspartic acid
- NAA
naphthalene-1-acetic acid
- NAAasp
N-[1-naphthylacetyl]-l-aspartic acid
- NPA
N-1-naphthylphthalamic acid
The authors thank Dr. M. Caboche (I.N.R.A, Versailles, France) for his generous gifts of some amide derivatives of 1-NAA, Mr. P. Varennes and Dr. B. Das (I.C.S.N., C.N.R.S., Gif-sur-Yvette, France) for recording and interpreting the mass spectra of NAA glucose ester, and Prof. P. Manigault (Institut des Sciences Végétales, Gif-sur-Yvette) for microscopy measurements of protoplast dimensions. This work was supported by funds from the C.N.R.S, I.N.R.A, and E.E.C. 相似文献
16.
Janaine Almeida Neto Daniel Amando Nery Katia Simoni Bezerra Lima Maria Eduarda Gomes da Cruz Silva Tarcísio Cícero de Lima Araújo Nathália Andrezza Carvalho de Souza Rodolfo Hideki Vicente Nishimura Camila de Souza Araújo Ana Paula de Oliveira Jackson Roberto Guedes da Silva Almeida Larissa Araújo Rolim 《化学与生物多样性》2023,20(3):e202201039
This article describes the phytochemical study of Cannabis sativa roots from northeastern Brazil. The dried plant material was pulverized and subjected to exhaustive maceration with ethanol at room temperature, obtaining the crude ethanolic extract (Cs-EEBR). The volatile compounds were analyzed by gas chromatography coupled with mass spectrometry (GC/MS), which allowed to identify 22 compounds by comparing the linear retention index (LRI), the similarity index (SI) and the fragmentation pattern of the constituents with the literature. By this technique the major compounds identified were: friedelan-3-one and β-sitosterol. In addition, two fractions were obtained from Cs-EEBR by classical column chromatography and preparative thin layer chromatography. These fractions were analyzed by NMR and IR and together with the mass spectrometry data allowed to identify the compounds: epifriedelanol, friedelan-3-one, β-sitosterol and stigmasterol. The study contributed to the phytochemical knowledge of Cannabis sativa, specifically the roots, as there are few reports on the chemical constituents of this part of the plant. 相似文献
17.
Coelho-Rocha Nina Dias de Jesus Luís Cláudio Lima Barroso Fernanda Alvarenga Lima da Silva Tales Fernando Ferreira Enio Gonçalves José Eduardo dos Santos Martins Flaviano de Oliveira Carvalho Rodrigo Dias Barh Debmalya Azevedo Vasco Ariston de Carvalho 《Probiotics and antimicrobial proteins》2023,15(1):160-174
Probiotics and Antimicrobial Proteins - Beneficial effects of Lactiplantibacillus plantarum strains have been widely reported. Knowing that the effects of probiotic bacteria are strain-dependent,... 相似文献
18.
E. S. Dias E. F. Araújo W. V. Guimarães R. M. C. Muchovej 《World journal of microbiology & biotechnology》1996,12(6):625-628
Experiments were performed with the mycorrhizal fungus Suillus granulatus to define the parameters for production and regeneration of protoplasts. Protoplasts were released at frequencies between 1 and 3×107/ml from mycelium 3 to 7 days old. The best osmotic stabilizer for protoplast release was MgSO4 (0.7 m). To optimize protoplast release and regeneration an enzyme (Novozym 234) concentration 1.7 mg/ml was chosen, with a digestion time of 1 to 2 h. Regenerated colonies formed mycorrhizae within 60 days after inoculation in Pinus caribaea var. hondurensis seedlings. 相似文献
19.
20.
A spontaneous melanotic ependymoma was observed in the brain of an adult female Goeldi's marmoset (Callimico goeldii). The mass completely occupied the left lateral ventricle, rupturing the fornix and corpus callosum, and compressing the adjacent neuropil. Special histochemical techniques, including melanin bleach, periodic acid-Schiff, Perls iron and phosphotungstic acid hematoxylin, demonstrated the neoplasms to be an ependymoma with a rare melanotic differentiation. 相似文献