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排序方式: 共有180条查询结果,搜索用时 0 毫秒
41.
Complexity of the heat stress response in plants 总被引:11,自引:0,他引:11
42.
Kaleigh Canfield Jiaqi Li Owen M. Wilkins Meghan M Morrison Matthew Ung Wendy Wells Charlotte R. Williams Karen T Liby Detlef Vullhorst Andres Buonanno Huizhong Hu Rachel Schiff Rebecca S Cook Manabu Kurokawa 《Cell cycle (Georgetown, Tex.)》2015,14(4):648-655
Approximately 25% of breast cancers overexpress and depend on the receptor tyrosine kinase ERBB2, one of 4 ERBB family members. Targeted therapies directed against ERBB2 have been developed and used clinically, but many patients continue to develop resistance to such therapies. Although much effort has been focused on elucidating the mechanisms of acquired resistance to ERBB2-targeted therapies, the involvement of ERBB4 remains elusive and controversial. We demonstrate that genetic ablation of ERBB4, but not ERBB1-3, led to apoptosis in lapatinib-resistant cells, suggesting that the efficacy of pan-ERBB inhibitors was, at least in part, mediated by the inhibition of ERBB4. Moreover, ERBB4 was upregulated at the protein level in ERBB2+ breast cancer cell lines selected for acquired lapatinib resistance in vitro and in MMTV-Neu mice following prolonged lapatinib treatment. Knockdown of ERBB4 caused a decrease in AKT phosphorylation in resistant cells but not in sensitive cells, suggesting that ERBB4 activated the PI3K/AKT pathway in lapatinib-resistant cells. Importantly, ERBB4 knockdown triggered apoptosis not only in lapatinib-resistant cells but also in trastuzumab-resistant cells. Our results suggest that although ERBB4 is dispensable for naïve ERBB2+ breast cancer cells, it may play a key role in the survival of ERBB2+ cancer cells after they develop resistance to ERBB2 inhibitors, lapatinib and trastuzumab. 相似文献
43.
44.
Viivi Majava Chaozhan Wang Matti Myllykoski Salla M. Kangas Sung Ung Kang Nobuhiro Hayashi Peter Baumgärtel Anthony M. Heape Gert Lubec Petri Kursula 《Amino acids》2010,39(1):73-74
Myelin basic protein (MBP) is present between the cytoplasmic leaflets of the compact myelin membrane in both the peripheral
and central nervous systems, and characterized to be intrinsically disordered in solution. One of the best-characterized protein
ligands for MBP is calmodulin (CaM), a highly acidic calcium sensor. We pulled down MBP from human brain white matter as the
major calcium-dependent CaM-binding protein. We then used full-length brain MBP, and a peptide from rodent MBP, to structurally
characterize the MBP–CaM complex in solution by small-angle X-ray scattering, NMR spectroscopy, synchrotron radiation circular
dichroism spectroscopy, and size exclusion chromatography. We determined 3D structures for the full-length protein–protein
complex at different stoichiometries and detect ligand-induced folding of MBP. We also obtained thermodynamic data for the
two CaM-binding sites of MBP, indicating that CaM does not collapse upon binding to MBP, and show that CaM and MBP colocalize
in myelin sheaths. In addition, we analyzed the post-translational modifications of rat brain MBP, identifying a novel MBP
modification, glucosylation. Our results provide a detailed picture of the MBP–CaM interaction, including a 3D model of the
complex between full-length proteins. 相似文献
45.
46.
Alain Noncovich Chad Priest Jane Ung Andrew P. Patron Guy Servant Paul Brust Nicole Servant Nathan Faber Hanghui Liu Nicole S. Gonsalves Tanya L. Ditschun 《Bioorganic & medicinal chemistry letters》2017,27(16):3931-3938
The paper presents the activity trends for a novel series of phenoxyacetyl amides as human TRPM8 receptor agonists. This series encompasses in vitro activity values ranging from the micromolar to the picomolar levels. Sensory evaluation of these molecules highlights their relevance as cooling agents for oral applications. The positive outcome of the complete evaluation of N-(1H-pyrazol-3-yl)-N-(thiophen-2-ylmethyl)-2-(p-tolyloxy)acetamide resulted in its approval for Generally Recognized As Safe (GRAS) status by the Flavor & Extract Manufacturer Association (FEMA) as FEMA 4809. 相似文献
47.
Activation and Tyrosine Phosphorylation of 44-kDa Mitogen-Activated Protein Kinase (MAPK) Induced by Electroconvulsive Shock in Rat Hippocampus 总被引:1,自引:0,他引:1
Ung Gu Kang Kyung Sue Hong Hee Yeon Jung Yong Sik Kim Yeon-Sun Seong Yun Chung Yang Joo-Bae Park 《Journal of neurochemistry》1994,63(5):1979-1982
Abstract: Electroconvulsive shock (ECS) has been reported to induce the phosphorylation and activation of 42-kDa, but not 44-kDa, mitogen-activated protein kinase (MAPK) in rat hippocampus. We studied the activation and tyrosine phosphorylation of MAPKs in rat brain after ECS. We observed the increase of the activities of both 42- and 44-kDa MAPKs in rat hippocampus after ECS. The activities reached peak at 2 min and returned to basal levels by 15 min after ECS. We also observed the increased phsophorylation on the tyrosine residue of 42-kDa MAPK in rat hippocampus after ECS, but not on that of 44-kDa MAPK. However, when we examined the immunoprecipitated 44-kDa MAPK, we could demonstrate that the tyrosine phosphorylation of 44-kDa MAPK at 2 min after ECS was markedly increased, in accordance with the increase of kinase activity. These results indicate that ECS induces the transient activation and tyrosine phosphorylation of 44-kDa MAPK, as well as 42-kDa MAPK, in rat hippocampus, although the amount of tyrosine phosphorylation is far less and the kinase activity is lower in 44-kDa MAPK than in 42-kDa MAPK. 相似文献
48.
Stable genetic transformation of Arabidopsis thaliana by Agrobacterium inoculation in planta 总被引:9,自引:0,他引:9
Seok So Chang Soon Ki Park Byung Chul Kim Bong Joong Kang Dal Ung Kim Hong Gil Nam 《The Plant journal : for cell and molecular biology》1994,5(4):551-558
Stable genetic transformation of Arabidopsis thaliana was achieved by simple in planta inoculation of Agrobacterium tumefaciens strain LBA4404 harboring a binary vector pBl121. The transformation procedure, which we call in planta transformation, involves severing of apical shoots at their bases, inoculation with Agrobacterium at the severed sites, and in planta generation of shoots from the severed sites. On average, 5.5% of the newly formed shoots produced transformed progenies. These progenies (T2 generation) contained T-DNA in the genome as examined by assaying the T-DNA encoded β-glucuronidase and kanamycin resistance and by genomic Southern blot analysis, the copy number of the T-DNAs in the Arabidopsis genome being single (33%) or multiple. The genetic behavior of the transformants examined at the T3 and T4 generations or with the F2 progenies of the outcrosses between transformants and wild-type plants showed that most of the inserted T-DNA are inherited in a Mendelian fashion. This procedure provides a new approach for simple and efficient transformation of A. thaliana, obviating the need for plant regeneration from tissue explants in vitro. 相似文献
49.
Miguel Burgos Noelia Fradejas Soledad Calvo Sung Ung Kang Pedro Tranque Gert Lubec 《Amino acids》2011,40(2):641-651
Astrocytes are glial cells in the central nervous system (CNS) that play key roles in brain physiology, controlling processes,
such as neurogenesis, brain energy metabolism and synaptic transmission. Recently, immune functions have also been demonstrated
in astrocytes, influencing neuronal survival in the course of neuroinflammatory pathologies. In this regard, PKCepsilon (PKCε)
is a protein kinase with an outstanding role in inflammation. Our previous findings indicating that PKCε regulates voltage-dependent
calcium channels as well as morphological stellation imply that this kinase controls multiple signalling pathways within astrocytes,
including those implicated in activation of immune functions. The present study applies proteomics to investigate new protein
targets of PKCε in astrocytes. Primary astrocyte cultures infected with an adenovirus that expresses constitutively active
PKCε were compared with infection controls. Two-dimensional gel electrophoresis clearly detected 549 spots in cultured astrocytes,
and analysis of differential protein expression revealed 18 spots regulated by PKCε. Protein identification by mass spectrometry
(nano-LC–ESI-MS/MS) showed that PKCε targets molecules with heterogeneous functions, including chaperones, cytoskeletal components
and proteins implicated in metabolism and signalling. These results support the notion that PKCε is involved in astrocyte
activation; also suggesting that multiple astrocyte-dependent processes are regulated by PKCε, including those associated
to neuroinflammation. 相似文献
50.
Targeted disruption of SPI3/Serpinb6 does not result in developmental or growth defects, leukocyte dysfunction, or susceptibility to stroke 下载免费PDF全文
Scarff KL Ung KS Nandurkar H Crack PJ Bird CH Bird PI 《Molecular and cellular biology》2004,24(9):4075-4082
Protease inhibitor 6 (PI-6/SERPINB6) is a widely expressed nucleocytoplasmic serpin. It inhibits granulocyte cathepsin G and neuronal neuropsin, and it is thought to protect cells from death caused by ectopic release or internalization of protease during stress such as infection or cerebral ischemia. To probe the biological functions of PI-6, we generated mice lacking its ortholog (SPI3/Serpinb6). SPI3-deficient mice developed normally and were fertile, and no abnormal pathology or increased sensitivity to cerebral ischemia was observed. There were no perturbations in leukocyte development or numbers, and recruitment of leukocytes to the peritoneal cavity was normal. SPI3-deficient mice were equally susceptible as wild-type mice to systemic Candida albicans infection, although there was a slight decrease in the ability of neutrophils from SPI3-deficient mice to kill C. albicans in vitro. Increased levels of a related inhibitor Serpinb1 (monocyte/neutrophil elastase inhibitor) in the tissues of targeted mice suggests that compensation by other serpins reduces the impact of SPI3 deficiency in these animals and may explain the lack of a more obvious phenotype. 相似文献