全文获取类型
收费全文 | 348篇 |
免费 | 27篇 |
出版年
2023年 | 1篇 |
2022年 | 3篇 |
2021年 | 11篇 |
2020年 | 4篇 |
2019年 | 8篇 |
2018年 | 18篇 |
2017年 | 15篇 |
2016年 | 22篇 |
2015年 | 24篇 |
2014年 | 18篇 |
2013年 | 19篇 |
2012年 | 34篇 |
2011年 | 26篇 |
2010年 | 11篇 |
2009年 | 13篇 |
2008年 | 21篇 |
2007年 | 17篇 |
2006年 | 21篇 |
2005年 | 27篇 |
2004年 | 17篇 |
2003年 | 10篇 |
2002年 | 10篇 |
2001年 | 2篇 |
2000年 | 8篇 |
1999年 | 2篇 |
1998年 | 2篇 |
1996年 | 2篇 |
1991年 | 1篇 |
1990年 | 2篇 |
1986年 | 1篇 |
1981年 | 1篇 |
1979年 | 1篇 |
1978年 | 2篇 |
1965年 | 1篇 |
排序方式: 共有375条查询结果,搜索用时 171 毫秒
21.
Markus K Muellner Barbara Mair Yasir Ibrahim Claudia Kerzendorfer Hannelore Lechtermann Claudia Trefzer Freya Klepsch André C Müller Ernestine Leitner Sabine Macho‐Maschler Giulio Superti‐Furga Keiryn L Bennett José Baselga Uwe Rix Stefan Kubicek Jacques Colinge Violeta Serra Sebastian MB Nijman 《Molecular systems biology》2015,11(2)
22.
23.
Lambrev PH Tsonev T Velikova V Georgieva K Lambreva MD Yordanov I Kovács L Garab G 《Photosynthesis research》2007,94(2-3):321-332
The kinetics of non-photochemical quenching (NPQ) of chlorophyll fluorescence was studied in pea leaves at different temperatures
between 5 and 25°C and during rapid jumps of the leaf temperature. At 5°C, NPQ relaxed very slowly in the dark and was sustained
for up to 30 min. This was independent of the temperature at which quenching was induced. Upon raising the temperature to
25°C, the quenched state relaxed within 1 min, characteristic for qE, the energy-dependent component of NPQ. Measurements
of the membrane permeability (ΔA515) in dark-adapted and preilluminated leaves and NPQ in the presence of dithiothreitol strongly suggest that the effect of
low temperature on NPQ was not because of limitation by the lumenal pH or the de-epoxidation state of the xanthophylls. These
data are consistent with the notion that the transition from the quenched to the unquenched state and vice versa involves
a structural reorganization in the photosynthetic apparatus. An eight-state reaction scheme for NPQ is proposed, extending
the model of Horton and co-workers (FEBS Lett 579:4201–4206, 2005), and a hypothesis is put forward concerning the nature of conformational changes associated with qE.
Electronic supplementary material The online version of this article (doi: ) contains supplementary material, which is available to authorized users. 相似文献
24.
Gourdet C Iribarren C Morin V Bustos P Puchi M Imschenetzky M 《Journal of cellular biochemistry》2007,101(1):1-8
Previously we have identified a cysteine-protease involved in male chromatin remodeling which segregates into the nuclei of the two blastomeres at the first cleavage division. Here we have investigated the fate of this protease during early embryogenesis by immunodetecting this protein with antibodies elicited against its N-terminal sequence. As shown in this report, the major 60 kDa active form of this protease was found to be present in the extracts of chromosomal proteins obtained from all developmental stages analyzed. In morula and gastrula the 70 kDa inactive precursor, which corresponds to the major form of the zymogen found in unfertilized eggs, was detected. In plutei larvas, the major 60 kDa form of this enzyme was found together with a higher molecular weight precursor (90 kDa) which is consistent with the less abundant zymogen primarily detected in unfertilized eggs. As reported here, either the active protease or its zymogens were visualized in most of the embryonic territories indicating that this enzyme lacks a specific pattern of spatial-temporal developmental segregation. Taken together our results indicate that this protease persists in the embryo and is ubiquitously distributed up to larval stages of development, either as an active enzyme and/or as an inactive precursor. These results suggest that this enzyme may display yet unknown functions during embryonic development that complement its role in male chromatin remodeling after fertilization. 相似文献
25.
The neuromuscular junction (NMJ) is a complex structure that efficiently communicates the electrical impulse from the motor neuron to the skeletal muscle to induce muscle contraction. Genetic and autoimmune disorders known to compromise neuromuscular transmission are providing further insights into the complexities of NMJ function. Congenital myasthenic syndromes (CMSs) are a genetically and phenotypically heterogeneous group of rare hereditary disorders affecting neuromuscular transmission. The understanding of the molecular basis of the different types of CMSs has evolved rapidly in recent years. Mutations were first identified in the subunits of the nicotinic acetylcholine receptor (AChR), but now mutations in ten different genes - encoding post-, pre- or synaptic proteins - are known to cause CMSs. Pathogenic mechanisms leading to an impaired neuromuscular transmission modify AChRs or endplate structure or lead to decreased acetylcholine synthesis and release. However, the genetic background of many CMS forms is still unresolved. A precise molecular classification of CMS type is of paramount importance for the diagnosis, counselling and therapy of a patient, as different drugs may be beneficial or deleterious depending on the molecular background of the particular CMS. 相似文献
26.
Martnez-Castillo Violeta Rodrguez-Troncoso Alma Paola de Jess Adolfo Tortolero-Langarica Jos Bautista-Guerrero Eric Padilla-Gamio Jacqueline Cupul-Magaa Amlcar Lev 《Hydrobiologia》2022,849(10):2395-2412
Hydrobiologia - Reef development occurs commonly under oligotrophic conditions that favor corals over competitors. In the Eastern Tropical Pacific (ETP), coral communities develop under highly... 相似文献
27.
28.
29.
Martinović J Dopsaj V Kotur-Stevuljević J Dopsaj M Vujović A Stefanović A Nešić G 《Journal of strength and conditioning research / National Strength & Conditioning Association》2011,25(5):1360-1367
The objectives of this study were to determine (a) if reactive oxygen metabolites (ROMs) are a reliable parameter for monitoring oxidative stress in athletes alone or in association with other parameters of oxidative stress and depending on whether antioxidant supplements are taken or not; (b) the level of oxidative stress in athletes before the competition season; and (c) if oxidative status could be improved in volleyball athletes. Sixteen women athletes (supplemented group) received an antioxidant cocktail containing vitamin E, vitamin C, zinc gluconate, and selenium as a dietary supplement during a 6-week training period, whereas 12 of them (control group) received no dietary supplement. Blood samples were taken before and after the training period. The following parameters were measured: ROMs, superoxide anion (O2??), malondialdehyde (MDA), advanced oxidation protein products (AOPP), lipid hydroperoxide (LOOH), biological antioxidative potential (BAP), paraoxonase activity toward paraoxon (POase) and diazoxon (DZOase), superoxide dismutase(SOD), total sulfydryl group concentration (SH groups), and lipid status. Reactive oxygen metabolites were taken as the dependent variable and MDA, O2??, AOPP, and LOOH as independent variables. In the group of athletes who have received supplementation, linear regression analysis revealed that the implemented model had a lower influence on dROMs (70.4 vs. 27.9%) after the training period. The general linear model showed significant differences between parameters before and after training/supplementation (Wilks' lambda = 0.074, F = 11.76, p < 0.01). At the partial level, significant increases in ROM levels (p <0.05, 95% confidence interval [CI]: 286-337), SOD activity (CI: 113-144), and BAP (CI: 2,388-2,580) (p < 0.01) were observed. The association between ROMs and other parameters of oxidative stress was reduced in athletes who received supplements. During the precompetition training period, treatment with dietary supplements prevented the depletion of antioxidative defense in volleyball athletes. 相似文献
30.
Veljkovic M Dopsaj V Dopsaj M Branch DR Veljkovic N Sakarellos-Daitsiotis MM Veljkovic V Glisic S Colombatti A 《PloS one》2011,6(11):e28304