全文获取类型
收费全文 | 10082篇 |
免费 | 912篇 |
国内免费 | 14篇 |
出版年
2023年 | 36篇 |
2022年 | 93篇 |
2021年 | 234篇 |
2020年 | 126篇 |
2019年 | 151篇 |
2018年 | 180篇 |
2017年 | 154篇 |
2016年 | 278篇 |
2015年 | 457篇 |
2014年 | 472篇 |
2013年 | 620篇 |
2012年 | 803篇 |
2011年 | 715篇 |
2010年 | 453篇 |
2009年 | 484篇 |
2008年 | 621篇 |
2007年 | 570篇 |
2006年 | 570篇 |
2005年 | 508篇 |
2004年 | 499篇 |
2003年 | 477篇 |
2002年 | 421篇 |
2001年 | 147篇 |
2000年 | 124篇 |
1999年 | 124篇 |
1998年 | 109篇 |
1997年 | 72篇 |
1996年 | 70篇 |
1995年 | 63篇 |
1994年 | 74篇 |
1993年 | 65篇 |
1992年 | 93篇 |
1991年 | 69篇 |
1990年 | 73篇 |
1989年 | 68篇 |
1988年 | 66篇 |
1987年 | 52篇 |
1986年 | 49篇 |
1985年 | 39篇 |
1984年 | 52篇 |
1983年 | 54篇 |
1982年 | 36篇 |
1981年 | 49篇 |
1980年 | 41篇 |
1979年 | 58篇 |
1977年 | 38篇 |
1976年 | 39篇 |
1975年 | 35篇 |
1974年 | 38篇 |
1973年 | 33篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
991.
Jean-Philippe Puyravaud Céline Dufour Subramanian Aravajy 《Journal of Biogeography》2003,30(7):1067-1080
Aim The objective of this study was to document succession from grassland thickets to rain forest, and to provide evidence for their potential as restoration tools. Location The Linganamakki region (State of Karnataka) of the Central Western Ghats of India. Method We selected thirty vegetation thickets ranging from 4 to 439 m2 in area in the vicinity of rain forest. The area of each small thicket was estimated as an oval using its maximum length and its maximum width. When the shape was irregular (mostly in large thickets) the limits of the thicket were mapped and the area calculated from the map. Plant species were identified, the number of individuals was estimated and their heights measured. Results There was a progression in the thickets from early to late successional species. Small thickets were characterized by ecotone species and savanna trees such as Catunaregam dumetorum. Savanna trees served as a nucleus for thicket formation. Colonizing species were mostly bird‐dispersed. As succession proceeded in larger thickets, the proportion of evergreen, late‐successional rain forest trees increased. The species composition of the large thickets differed depending on the species composition of reproductive adults in the nearby forested areas. The species within small thickets were also found in the large thickets. The nestedness in species composition suggested that species turnover was deterministic based on thicket size. Human disturbance (leaf and wood collection by the local populations) affected the species composition and the species–area relationship of thickets. Main conclusions Vegetation thickets are nodal centres for rain forest colonization within grasslands. They expand and replace savanna. Early successional bird‐dispersed species established around savanna trees followed by late‐successional rain forest trees dispersed from the nearby forest by birds. Restoration programmes that reproduce natural successional processes such as those observed in thickets will be more successful and less expensive than the methods currently being employed, where trees are individually planted in grassland. Wood harvesting is the only factor that prevents thicket growth and coalescence and hampers forest expansion. 相似文献
992.
Peter W. Cullen Richard H. Norris Vincent H. Resh Trefor B. Reynoldson David M. Rosenberg Michael T. Barbour 《Freshwater Biology》1999,42(1):131-142
1. Collaboration means actively working together to achieve things which could not be done alone. This article attempts to provide an overall, unified, guiding framework for collaboration in freshwater ecology by discussing aspects of collaboration at individual and organizational levels, and addressing international linkages.
2. The essential elements of collaboration are communication and trust, and effective project management. Barriers to effective collaboration include competition, organizational cultures and organizational instabilities.
3. The success of collaboration can be measured by tangible benefits such as increased numbers of peer-reviewed publications, the production of working models and a number of intangible benefits.
4. Interactions between individuals lie at the heart of an effective collaboration; organizational arrangements should facilitate this interaction. Some governments are encouraging collaboration to increase cost efficiency and allocate accountability. This trend should continue on an international level.
5. Collaboration is a key to future research in freshwater ecology. 相似文献
2. The essential elements of collaboration are communication and trust, and effective project management. Barriers to effective collaboration include competition, organizational cultures and organizational instabilities.
3. The success of collaboration can be measured by tangible benefits such as increased numbers of peer-reviewed publications, the production of working models and a number of intangible benefits.
4. Interactions between individuals lie at the heart of an effective collaboration; organizational arrangements should facilitate this interaction. Some governments are encouraging collaboration to increase cost efficiency and allocate accountability. This trend should continue on an international level.
5. Collaboration is a key to future research in freshwater ecology. 相似文献
993.
994.
995.
The behavior of pollen grains within the airspace around Ephedra trifurca is described. Vectoral analyses of pollen grains moving around stems and ovules indicate a complex pattern of directional and magnitudinal changes in trajectories that can be related to the geometries of surfaces that obstruct airflow. Pollen grains, passing around cylindrical stems oriented normal to the direction of ambient airflow, are deflected in circumrotating non-laminar flow-patterns. Stems tilted downwind deflect pollen grains into trajectories along leeward surfaces of stems. These trajectories travel acropetally in a spiraling pattern and may intersect airflow patterns created around and by ovules. Computer analyses of pollen motion in the vicinity of ovules indicate that pollen vector-direction is highly canalized and directed toward micropyles. Within the immediate vicinity of micropyles, which produce pollination droplets, analyses indicate that the magnitudinal variance of pollen grain vectors is high (spanning three orders of magnitude). This variance coincides with dramatic changes in the local Reynolds numbers, resulting in a localized region around the micropyle in which neither viscous nor inertial forces predominate. Based on additional aerodynamics parameters (vector curl, vector-divergence, and vector curl-differential) it is shown that the region around the pollination-droplet is characteristically a “pollen sink” (pollen grains collect in this airspace) in which abrupt changes can occur in the angular momenta of airborne pollen grains. These aerodynamic analyses suggest that the morphology of ovules and the stems to which they are attached facilitates pollen capture by creating an aerodynamic “singularity” (= a unique region) around the pollination-droplet. 相似文献
996.
Warwick F. Vincent 《Journal of phycology》1979,15(4):429-434
An inverse linear relationship between chlorophyll fluorescence yield (R) and light intensity was recorded in the near-surface waters of six lakes (New Zealand, England) of greatly different trophic status and phytoplankton species composition. This surface depression of R values could be removed by incubation of samples in dim light or darkness and was not observed in situ below a threshold irradiance (146 μEin ·m?2·s?1 for Lake Taupo, New Zealand). The time course of chlorophyll fluorescence depression and recovery in response to light treatment was measured in samples from Lake Windermere (England). Fluorescence exponentially decreased upon exposure to bright light and the response was 100% (5 m samples) or 83% (dim light-adapted 0 m samples) complete within 2 min. An increase in R values in the dim light occurred after a lag of 60 s and the rate of increase decreased exponentially with time. Full recovery took 15 min or more. Deep (6.5 m) populations from Lake Windermere exhibited large, time-dependent variations in chlorophyll fluorescence over the first 25 s of exposure to 450 nm light, whereas surface populations did not. These data were interpreted in terms of decreased spillover from PSII to PSI with increasing depth, to a minimum at the threshold light intensity below which cells are in light state 1. 相似文献
997.
998.
Translocations, deletions, and chromosome fusions are frequent events seen in cancers with genome instability. Here we analyzed 358 genome rearrangements generated in Saccharomyces cerevisiae selected by the loss of the nonessential terminal segment of chromosome V. The rearrangements appeared to be generated by both nonhomologous end joining and homologous recombination and targeted all chromosomes. Fifteen percent of the rearrangements occurred independently more than once. High levels of specific classes of rearrangements were isolated from strains with specific mutations: translocations to Ty elements were increased in telomerase-defective mutants, potential dicentric translocations and dicentric isochromosomes were associated with cell cycle checkpoint defects, chromosome fusions were frequent in strains with both telomerase and cell cycle checkpoint defects, and translocations to homolog genes were seen in strains with defects allowing homoeologous recombination. An analysis of human cancer-associated rearrangements revealed parallels to the effects that strain genotypes have on classes of rearrangement in S. cerevisiae. 相似文献
999.
Inactivation of Ku-mediated end joining suppresses mec1Delta lethality by depleting the ribonucleotide reductase inhibitor Sml1 through a pathway controlled by Tel1 kinase and the Mre11 complex
下载免费PDF全文
![点击此处可从《Molecular and cellular biology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Corda Y Lee SE Guillot S Walther A Sollier J Arbel-Eden A Haber JE Géli V 《Molecular and cellular biology》2005,25(23):10652-10664
RAD53 and MEC1 are essential Saccharomyces cerevisiae genes required for the DNA replication and DNA damage checkpoint responses. Their lethality can be suppressed by increasing the intracellular pool of deoxynucleotide triphosphates. We report that deletion of YKU70 or YKU80 suppresses mec1Delta, but not rad53Delta, lethality. We show that suppression of mec1Delta lethality is not due to Ku--associated telomeric defects but rather results from the inability of Ku- cells to efficiently repair DNA double strand breaks by nonhomologous end joining. Consistent with these results, mec1Delta lethality is also suppressed by lif1Delta, which like yku70Delta and yku80Delta, prevents nonhomologous end joining. The viability of yku70Delta mec1Delta and yku80Delta mec1Delta cells depends on the ATM-related Tel1 kinase, the Mre11-Rad50-Xrs2 complex, and the DNA damage checkpoint protein Rad9. We further report that this Mec1-independent pathway converges with the Rad53/Dun1-regulated checkpoint kinase cascade and leads to the degradation of the ribonucleotide reductase inhibitor Sml1. 相似文献
1000.
Expression of constitutively stable hybrid hypoxia-inducible factor-1alpha protects cultured rat cardiomyocytes against simulated ischemia-reperfusion injury 总被引:13,自引:0,他引:13