全文获取类型
收费全文 | 9758篇 |
免费 | 881篇 |
国内免费 | 15篇 |
出版年
2023年 | 36篇 |
2022年 | 73篇 |
2021年 | 229篇 |
2020年 | 120篇 |
2019年 | 148篇 |
2018年 | 174篇 |
2017年 | 155篇 |
2016年 | 271篇 |
2015年 | 446篇 |
2014年 | 458篇 |
2013年 | 594篇 |
2012年 | 771篇 |
2011年 | 693篇 |
2010年 | 444篇 |
2009年 | 470篇 |
2008年 | 612篇 |
2007年 | 556篇 |
2006年 | 551篇 |
2005年 | 483篇 |
2004年 | 487篇 |
2003年 | 471篇 |
2002年 | 421篇 |
2001年 | 137篇 |
2000年 | 125篇 |
1999年 | 113篇 |
1998年 | 103篇 |
1997年 | 65篇 |
1996年 | 68篇 |
1995年 | 59篇 |
1994年 | 67篇 |
1993年 | 62篇 |
1992年 | 84篇 |
1991年 | 64篇 |
1990年 | 66篇 |
1989年 | 74篇 |
1988年 | 62篇 |
1987年 | 51篇 |
1986年 | 50篇 |
1985年 | 45篇 |
1984年 | 53篇 |
1983年 | 54篇 |
1982年 | 35篇 |
1981年 | 43篇 |
1980年 | 44篇 |
1979年 | 55篇 |
1977年 | 32篇 |
1976年 | 36篇 |
1975年 | 33篇 |
1974年 | 38篇 |
1973年 | 30篇 |
排序方式: 共有10000条查询结果,搜索用时 31 毫秒
991.
Warwick F. Vincent 《Journal of phycology》1979,15(4):429-434
An inverse linear relationship between chlorophyll fluorescence yield (R) and light intensity was recorded in the near-surface waters of six lakes (New Zealand, England) of greatly different trophic status and phytoplankton species composition. This surface depression of R values could be removed by incubation of samples in dim light or darkness and was not observed in situ below a threshold irradiance (146 μEin ·m?2·s?1 for Lake Taupo, New Zealand). The time course of chlorophyll fluorescence depression and recovery in response to light treatment was measured in samples from Lake Windermere (England). Fluorescence exponentially decreased upon exposure to bright light and the response was 100% (5 m samples) or 83% (dim light-adapted 0 m samples) complete within 2 min. An increase in R values in the dim light occurred after a lag of 60 s and the rate of increase decreased exponentially with time. Full recovery took 15 min or more. Deep (6.5 m) populations from Lake Windermere exhibited large, time-dependent variations in chlorophyll fluorescence over the first 25 s of exposure to 450 nm light, whereas surface populations did not. These data were interpreted in terms of decreased spillover from PSII to PSI with increasing depth, to a minimum at the threshold light intensity below which cells are in light state 1. 相似文献
992.
993.
Translocations, deletions, and chromosome fusions are frequent events seen in cancers with genome instability. Here we analyzed 358 genome rearrangements generated in Saccharomyces cerevisiae selected by the loss of the nonessential terminal segment of chromosome V. The rearrangements appeared to be generated by both nonhomologous end joining and homologous recombination and targeted all chromosomes. Fifteen percent of the rearrangements occurred independently more than once. High levels of specific classes of rearrangements were isolated from strains with specific mutations: translocations to Ty elements were increased in telomerase-defective mutants, potential dicentric translocations and dicentric isochromosomes were associated with cell cycle checkpoint defects, chromosome fusions were frequent in strains with both telomerase and cell cycle checkpoint defects, and translocations to homolog genes were seen in strains with defects allowing homoeologous recombination. An analysis of human cancer-associated rearrangements revealed parallels to the effects that strain genotypes have on classes of rearrangement in S. cerevisiae. 相似文献
994.
Inactivation of Ku-mediated end joining suppresses mec1Delta lethality by depleting the ribonucleotide reductase inhibitor Sml1 through a pathway controlled by Tel1 kinase and the Mre11 complex 下载免费PDF全文
Corda Y Lee SE Guillot S Walther A Sollier J Arbel-Eden A Haber JE Géli V 《Molecular and cellular biology》2005,25(23):10652-10664
RAD53 and MEC1 are essential Saccharomyces cerevisiae genes required for the DNA replication and DNA damage checkpoint responses. Their lethality can be suppressed by increasing the intracellular pool of deoxynucleotide triphosphates. We report that deletion of YKU70 or YKU80 suppresses mec1Delta, but not rad53Delta, lethality. We show that suppression of mec1Delta lethality is not due to Ku--associated telomeric defects but rather results from the inability of Ku- cells to efficiently repair DNA double strand breaks by nonhomologous end joining. Consistent with these results, mec1Delta lethality is also suppressed by lif1Delta, which like yku70Delta and yku80Delta, prevents nonhomologous end joining. The viability of yku70Delta mec1Delta and yku80Delta mec1Delta cells depends on the ATM-related Tel1 kinase, the Mre11-Rad50-Xrs2 complex, and the DNA damage checkpoint protein Rad9. We further report that this Mec1-independent pathway converges with the Rad53/Dun1-regulated checkpoint kinase cascade and leads to the degradation of the ribonucleotide reductase inhibitor Sml1. 相似文献
995.
Expression of constitutively stable hybrid hypoxia-inducible factor-1alpha protects cultured rat cardiomyocytes against simulated ischemia-reperfusion injury 总被引:13,自引:0,他引:13
996.
Tang V Dhirapong A Yabes AP Weiss RH 《American journal of physiology. Cell physiology》2005,289(1):C199-C206
Atherosclerosis, now considered an inflammatory process, is the leading cause of death in the Western world and is manifested by a variety of diseases in multiple organ systems. Because of its prevalence and associated morbidity, novel therapies directed at arresting this progressive process are urgently needed. The inflammatory mediator TNF-, which is known to contribute to apoptosis in vascular smooth muscle cells, has been shown to be intimately involved in the atherosclerotic process, being present at elevated levels in human atheroma as well as possibly being responsible for plaque rupture, a clinically devastating event. In light of our earlier finding that p73 is a proapoptotic protein in vascular smooth muscle cells, which are involved in plaque progression as well as rupture, we asked whether TNF- mediates apoptosis in these cells through p73. We now show that p73 is present in spindle-shaped cells within human atheroma, and p73, an isoform that is pivotal in both apoptosis and growth suppression, is induced in vascular smooth muscle cells in vitro by serum but not by PDGF-BB. In addition, TNF-, when added to these cells in the presence of serum-containing media, increases p73 expression and causes apoptosis in both rat and human vascular smooth muscle cells. Inhibition of p73 activity with a dominant inhibitory NH2-terminally deleted p73 plasmid results in markedly decreased TNF--induced apoptosis. Thus p73 is likely a mediator of the apoptotic effect of TNF- in the vasculature, such that future targeting of the p73 isoforms may ultimately prove useful in novel atherosclerosis therapies. atherosclerosis; inflammation; plaque 相似文献
997.
Regulatory elements of the melanocortin 1 receptor 总被引:1,自引:0,他引:1
998.
Expanded granular sludge bed-anaerobic filter (EGSB-AF) bioreactors were operated at 15 degrees C for the treatment of 2,4,6-trichlorophenol (TCP)-containing volatile fatty acid (VFA)-based wastewaters. The seed sludge used as inoculum for the control (no TCP) and test reactor was unexposed to chlorophenols (CPs) prior to the 425-day trial. TCP supplementation to the feed at 50 mg TCPl(-1) partially inhibited the anaerobic degradation of the VFA feed measured as COD removal efficiency. However, the withdrawal and subsequent application of stepwise increments to the TCP loading resulted in steady COD removal. Terminal restriction fragment length polymorphism analysis showed Methanosaeta-like Archaea in the control reactor over the experimental period. Different methanogenic populations were detected in the test reactor and responded to the changes in feed composition. Bacterial community analyses indicated changes in the community structure over time and suggested the presence of Campylobacter-like, Acidimicrobium-like and Heliophilum-like organisms in the samples. TCP mineralisation was by a reductive dechlorination pathway through 2,4-dichlorophenol (DCP) and 4-chlorophenol (4-CP) or 2-chlorophenol (2-CP). CP degradation rates in sludge granules from the lower chamber of the hybrid EGSB-AF reactor was in the order TCP > DCP > 4-CP > 2-CP. However, a biodegradability order of lower CPs > TCP was observed in fixed-film biomass taken from the upper reactor chamber, thus reflecting the role of this reactor section in the metabolism of residual lower CPs from the lower sludge-bed stage of operation. 相似文献
999.
Greggio E Bergantino E Carter D Ahmad R Costin GE Hearing VJ Clarimon J Singleton A Eerola J Hellström O Tienari PJ Miller DW Beilina A Bubacco L Cookson MR 《Journal of neurochemistry》2005,93(1):246-256
Tyrosinase is a key enzyme in the synthesis of melanin in skin and hair and has also been proposed to contribute to the formation of neuromelanin (NM). The presence of NM, which is biochemically similar to melanin in peripheral tissues, identifies groups of neurons susceptible in Parkinson's disease (PD). Whether tyrosinase is beneficial or detrimental to neurons is unclear; whilst the enzyme activity of tyrosinase generates dopamine-quinones and other oxidizing compounds, NM may form a sink for such radical species. In the present study, we demonstrated that tyrosinase is expressed at low levels in the human brain. We found that mRNA, protein and enzyme activity are all present but at barely detectable levels. In cell culture systems, expression of tyrosinase increases neuronal susceptibility to oxidizing conditions, including dopamine itself. We related these in vitro observations to the human disease by assessing whether there was any genetic association between the gene encoding tyrosinase and idiopathic PD. We found neither genotypic or haplotypic association with three polymorphic markers of the gene. This argues against a strong genetic association between tyrosinase and PD, although the observed contribution to cellular toxicity suggests that a biochemical association is likely. 相似文献
1000.
The characteristics governing the dynamics of populations can evolve and this evolution can either be towards stability or chaos. Yet it is not obvious how or why such population characteristics can evolve through selection on individuals. In this paper we construct a mathematical model, inspired by experimental results, illustrating the dynamics of a population of competing Drosophila. We demonstrate how selection of life history characteristics and stability influence one another as a population interacts with its environment. We generalize this result and show that population stability can evolve as a consequence of selection on individuals. 相似文献