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71.
Zárate G Morata De Ambrosini V Perez Chaia A González S 《Canadian journal of microbiology》2002,48(5):449-457
Adhesion to the intestinal mucosa is generally considered an important property of probiotic microorganisms and has been related to many of their health benefits. This study investigated some factors that could affect or be involved in the adherence of Propionibacterium acidipropionici CRL 1198, a dairy strain with suggested probiotic effects and high adherence in vitro and in vivo to intestinal epithelial cells. In vitro adhesion of propionibacteria was decreased by gastric digestion but not affected by bile and pancreatic enzymes. Adherence was also decreased by pretreatment of bacterial cells with protease, sodium metaperiodate, and trichloroacetic acid, revealing that different features of the cell surface, like protein factors, carbohydrates, and teichoic acids, are involved in the process. Adherence to intestinal epithelial cells was enhanced by calcium and was dependent on other divalent cations. Adhesion to intestinal mucus was also demonstrated. The results should explain the metabolic effects in the host previously obtained with this strain and support the potential of Propionibacterium for development of new probiotics. 相似文献
72.
Thioredoxin specifically cross-desensitizes monocytes to MCP-1 总被引:1,自引:0,他引:1
Pagliei S Ghezzi P Bizzarri C Sabbatini V Frascaroli G Sozzani S Caselli G Bertini R 《European cytokine network》2002,13(2):261-267
Thioredoxin (Trx) is a protein disulfide oxidoreductase which can be secreted and acts as a cytokine. As we recently reported that Trx is chemotactic, we investigated whether it desensitizes monocytes or PMN to other chemokines. Preincubation for 15 min with Trx inhibited the chemotactic response of monocytes to MCP-1, but not to fMLP. This effect was independent of whether Trx was present during the chemotaxis assay or only during the preincubation. Preincubation (5 min) with Trx also inhibited the increase in intracellular Ca(2+) induced by MCP-1 in monocytes, but not that induced by fMLP. Preincubation with Trx did not affect the chemotactic response induced in PMN by IL-8. The inhibition of chemotactic and Ca(2+) responses to MCP-1 in monocytes was not due to a down-regulation of the MCP-1 receptor, as shown by receptor binding studies. The Ca(2+) response to MCP-1 was also inhibited by Trx in a CCR2-transfected cell line. It is suggested that Trx inhibits monocyte responses to chemokines by acting downstream of the chemokine receptors. Since there are high concentrations of circulating Trx in infection and inflammatory diseases, this might act as an inhibitor of monocyte migration in vivo. 相似文献
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74.
Christa Kietz Aravind K. Mohan Vilma Pollari Ida-Emma Tuominen Paulo S. Ribeiro Pascal Meier Annika Meinander 《Cell death and differentiation》2022,29(1):28
The Drosophila IAP protein, Diap2, is a key mediator of NF-κB signalling and innate immune responses. Diap2 is required for both local immune activation, taking place in the epithelial cells of the gut and trachea, and for mounting systemic immune responses in the cells of the fat body. We have found that transgenic expression of Diap2 leads to a spontaneous induction of NF-κB target genes, inducing chronic inflammation in the Drosophila midgut, but not in the fat body. Drice is a Drosophila effector caspase known to interact and form a stable complex with Diap2. We have found that this complex formation induces its subsequent degradation, thereby regulating the amount of Diap2 driving NF-κB signalling in the intestine. Concordantly, loss of Drice activity leads to accumulation of Diap2 and to chronic intestinal inflammation. Interestingly, Drice does not interfere with pathogen-induced signalling, suggesting that it protects from immune responses induced by resident microbes. Accordingly, no inflammation was detected in transgenic Diap2 flies and Drice-mutant flies reared in axenic conditions. Hence, we show that Drice, by restraining Diap2, halts unwanted inflammatory signalling in the intestine.Subject terms: Ubiquitins, Gene regulation, Antimicrobial responses, Chronic inflammation, Signal transduction 相似文献
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Prado MA Alves-Silva J Magalhães AC Prado VF Linden R Martins VR Brentani RR 《Journal of neurochemistry》2004,88(4):769-781
The glycosylphosphatidylinositol (GPI)-anchored cellular prion protein (PrPc) has a fundamental role in prion diseases. Intracellular trafficking of PrPc is important in the generation of protease resistant PrP species but little is known of how endocytosis affects PrPc function. Here, we discuss recent experiments that have illuminated how PrPc is internalized and what are the possible destinations taken by the protein. Contrary to what would be expected for a GPI-anchored protein there is increasing evidence that clathrin-mediated endocytosis and classical endocytic organelles participate in PrPc trafficking. Moreover, the N-terminal domain of PrPc may be involved in sorting events that can direct the protein during its intracellular journey. Indeed, the concept that the GPI-anchor determines PrPc trafficking has been challenged. Cellular signaling can be triggered or be regulated by PrPc and we suggest that endocytosis of PrPc may influence signaling in several ways. Definition of the processes that participate in PrPc endocytosis and intracellular trafficking can have a major impact on our understanding of the mechanisms involved in PrPc function and conversion to protease resistant conformations. 相似文献
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Kristin Kruse Christina Janko Vilma Urbonaviciute Claudia T. Mierke Thomas H. Winkler Reinhard E. Voll Georg Schett Luis E. Muñoz Martin Herrmann 《Apoptosis : an international journal on programmed cell death》2010,15(9):1098-1113
Systemic lupus erythematosus (SLE) is a complex disease resulting from inflammatory responses of the immune system against
several autoantigens. Inflammation is conditioned by the continuous presence of autoantibodies and leaked autoantigens, e.g.
from not properly cleared dying and dead cells. Various soluble molecules and biophysical properties of the surface of apoptotic
cells play significant roles in the appropriate recognition and further processing of dying and dead cells. We exemplarily
discuss how Milk fat globule epidermal growth factor 8 (MFG-E8), biophysical membrane alterations, High mobility group box 1
(HMGB1), C-reactive protein (CRP), and anti-nuclear autoantibodies may contribute to the etiopathogenesis of the disease.
Up to date knowledge about these key elements may provide new insights that lead to the development of new treatment strategies
of the disease. 相似文献
80.
Pedro Ordunez Elisa Prieto-Lara Vilma Pinheiro Gawryszewski Anselm J. M. Hennis Richard S. Cooper 《PloS one》2015,10(10)