全文获取类型
收费全文 | 928篇 |
免费 | 44篇 |
国内免费 | 1篇 |
出版年
2023年 | 8篇 |
2022年 | 18篇 |
2021年 | 29篇 |
2020年 | 12篇 |
2019年 | 25篇 |
2018年 | 31篇 |
2017年 | 30篇 |
2016年 | 36篇 |
2015年 | 45篇 |
2014年 | 67篇 |
2013年 | 71篇 |
2012年 | 82篇 |
2011年 | 88篇 |
2010年 | 59篇 |
2009年 | 41篇 |
2008年 | 34篇 |
2007年 | 50篇 |
2006年 | 30篇 |
2005年 | 27篇 |
2004年 | 29篇 |
2003年 | 25篇 |
2002年 | 23篇 |
2001年 | 8篇 |
2000年 | 10篇 |
1999年 | 3篇 |
1997年 | 4篇 |
1996年 | 5篇 |
1994年 | 5篇 |
1992年 | 8篇 |
1991年 | 8篇 |
1990年 | 4篇 |
1989年 | 3篇 |
1988年 | 4篇 |
1987年 | 2篇 |
1986年 | 3篇 |
1985年 | 8篇 |
1984年 | 10篇 |
1983年 | 2篇 |
1982年 | 2篇 |
1981年 | 2篇 |
1979年 | 2篇 |
1978年 | 1篇 |
1976年 | 2篇 |
1974年 | 1篇 |
1973年 | 1篇 |
1972年 | 4篇 |
1970年 | 1篇 |
1969年 | 3篇 |
1967年 | 2篇 |
1950年 | 1篇 |
排序方式: 共有973条查询结果,搜索用时 46 毫秒
251.
Manjari Mishra Subhash Chandra Vikash C. Pandey Babu L. Tekwani 《Cell biochemistry and function》1997,15(4):229-235
The pathophysiological impact of infections with chloroquine-susceptible (CQS) and chloroquine-resistant (CQR) strains of Plasmodium berghei in Mastomys natalensis was studied with respect to changes in polyamine profiles in various tissues. Both CQS and CQR infections produced similar changes in polyamine profiles of various tissues. Maximum increase was recorded in spleen followed by liver and lungs. Renal, cardiac and cerebral tissues did not register significant changes. An increase in spermidine level was more prominent as compared to putrescine and spermine, leading to an overall increase in spermidine/spermine ratio. This ratio is an important index of cellular proliferation. Liver did not show considerable change in the activities of ornithine decarboxylase and S-adenosyl methionine decarboxylase, the regulatory enzymes of the polyamine biosynthetic pathway. Spleen however, registered marked induction of both the enzymes which was more prominent in the CQS infection than CQR. Normal erythrocytes contained traces of polyamine while the erythrocytes loaded with P. berghei parasites exhibited appreciably higher polyamine levels. Spermidine was detected in about five-fold higher concentrations than putrescine and spermine which were detected in equimolar levels. Again, CQS as well as CQR P. berghei, exhibited qualitatively and quantitatively similar polyamine profiles thus ruling out a role of polyamines in CQ-resistance in malaria. © 1997 John Wiley & Sons, Ltd. 相似文献
252.
Jagadeeshwar R. Rao Ashok K. Jha Ravindra K. Rawal Ashoke Sharon Craig W. Day Dale L. Barnard Donald F. Smee Chung K. Chu 《Bioorganic & medicinal chemistry letters》2010,20(8):2601-2604
Enantiomerically pure cyclopentyl cytosine [(?)-carbodine 1] was synthesized from d-ribose and evaluated for its anti-influenza activity in vitro in comparison to the (+)-carbodine, (±)-carbodine and ribavirin. (?)-Carbodine 1 exhibited potent antiviral activity against various strains of influenza A and B viruses. 相似文献
253.
The following article from Plant Biology, “Growth, ion homeostasis, photosynthesis and photosystem II efficiency of an obligate halophyte, Salicornia brachiata, under increasing salinity (p)” by A. K. Parida, C. M. Hari Kishore & B. Jha, published online on 1 April 2010 (DOI: 10.1111/j.1438‐8677.2010.00336.x ) in Wiley InterScience ( http://www.interscience.wiley.com ), has been retracted by agreement between the authors, the journal Editor‐in‐Chief, Heinz Rennenberg and Blackwell Publishing Ltd. The retraction has been agreed due to concern about overlap with the article “Relationship between the photosynthetic activity and the performance of Cakile maritima after long‐term salt treatment” by Ahmed Debez, Hans‐Werner Koyro, Claude Grignon, Chedly Abdelly, Bernhard Huchzermeyer published in Physiologia Plantarum (2008) vol. 133, 373‐385. 相似文献
254.
255.
Samanta S Srikanth K Banerjee S Debnath B Gayen S Jha T 《Bioorganic & medicinal chemistry》2004,12(6):1413-1423
Cancer is a major killer disease throughout human history. Thus, cancer becomes a major point of interest in life science. It was proved that cancer is a nitrogen trap and tumor cells are avid glutamine consumers. The non-essential amino acid glutamine, which is a glutamic acid derivative, supplies its amide nitrogen to tumor cells in the biosynthesis of purine and pyrimidine bases of nucleic acids as well as takes part in protein synthesis. Based on these and in continuation of our composite programme of development of new potential anticancer agents through rational drug design, 17 new 5-N-Substituted-2-(substituted benzenesulphonyl) glutamines were selected for synthesis. These compounds as well as 36 earlier synthesized glutamine analogues were screened for antitumor activity using percentage inhibition of tumor cell count as the activity parameter. QSAR study was performed with 53 compounds in order to design leads with increased effectiveness for antitumor activity using both physicochemical and topological parameters. QSAR study showed that steric effect on the aromatic ring is conducive to the activity. n-butyl substitution on aliphatic side chain and atom no 12 is important for antitumor activity of glutamine analogues. 相似文献
256.
Continuing our search for newer oestrogen agonists or antagonists and extending our work on the exploration of benzopyran related compounds, some new tricyclic molecules bridged between the active molecules of 3,4-diaryl chroman and 2,3-diaryl benzopyrans have been synthesised. Structural modifications at different positions with elements known to impart agonist or antagonist activities have been carried out to prepare the desired molecules. The target compounds were screened for their anti-osteoporotic (agonist) and anti-uterotrophic (antagonist) activities and were found to be moderately active. 相似文献
257.
A central issue in protein folding is the degree to which each residue's backbone conformational preferences stabilize the native state. We have studied the conformational preferences of each amino acid when the amino acid is not constrained to be in a regular secondary structure. In this large but highly restricted coil library, the backbone preferentially adopts dihedral angles consistent with the polyproline II conformation rather than alpha or beta conformations. The preference for the polyproline II conformation is independent of the degree of solvation. In conjunction with a new masking procedure, the frequencies in our coil library accurately recapitulate both helix and sheet frequencies for the amino acids in structured regions, as well as polyproline II propensities. Therefore, structural propensities for alpha-helices and beta-sheets and for polyproline II conformations in unfolded peptides can be rationalized solely by local effects. In addition, these propensities are often strongly affected by both the chemical nature and the conformation of neighboring residues, contrary to the Flory isolated residue hypothesis. 相似文献
258.
Using purified N-terminal NBD (NBD-512) domain of Cdr1p, a major multidrug extrusion pump of human pathogenic yeast Candida albicans, we show the relevance of the unique positioning of an atypical Trp326 residue. Similar to Cys193 in Walker A, Trp326 in the Walker B motif of Cdr1p is also a conserved feature of other fungal ATP Binding Cassette (ABC) transporters. By employing fluorescence spectroscopy, chemical modification, and site-directed mutagenesis, we demonstrate that of the five Trp residues in the NBD-512 domain, Trp326 alone is important for nucleotide binding and subsequent conformational changes within the domain. Furthermore, mutation of Trp326 to Ala results in an increased K(M) without appreciably affecting V(max) of ATPase activity. Thus, Trp326 in NBD-512 appears to be important for nucleotide binding and not for its hydrolysis. Additionally, the role of Trp326 in ATP binding is independent of the presence of the adjacent well-conserved Asp327 residue which, like Cys193, has a catalytic role in ATP hydrolysis. Considering that Trp326 of Cdr1p is a typical feature of fungal transporters alone, our study suggests that these ABC transporters may reflect mechanistic differences with regard to nucleotide binding and hydrolysis as compared to their counterparts of non-fungal origin. 相似文献
259.
From the roots and rhizomes of Helleborus macranthus a new glycoside, macranthoside I, has been isolated and shown to have the structure 25(27)-dehydro-5β-furostan-3β,22,26-triol-3-O-β-d-glucopyranosyl(1 → 6)-β-d-glucopyranoside, 26-O-β-d-glucopyranoside. 相似文献
260.
Sah NK Singh SN Sahdev S Banerji S Jha V Khan Z Hasnain SE 《Journal of biosciences》2005,30(4):499-505
Selaginella bryopteris is a lithophyte with remarkable ressurection capabilities. It is full of medicinal properties, hence also known as ‘Sanjeevani’ (one that infuses life). For lack of credible scientific evidence the plant is not in active use as a medicinal herb. We
provide scientific evidence for whyS. bryopteris is known as ‘Sanjeevani’.
The aqueous extract ofS. bryopteris possesses growth-promoting activity as well as protective action against stress-induced cell death in a number of experimental
cell systems including mammalian cells. Treatment of the cells in culture with 10% aqueous extract enhanced cell growth by
about 41% in Sf9 cells and 78% in mammalian cells. Pre-treatment of cells with the Selaginella extract (SE) (1-2x5%) protected
against oxidative stress (H2O2)-induced cell death. The killing potential of ultra violet (UV) was also significantly reduced
when the cells were pre-treated with SE for 1 h. Thermal radiation suppressed cell growth by about 50%. Pre-treatment of cells
with SE for 1 h afforded complete protection against heat-induced growth suppression. SE may possess anti-stress and antioxidant
activities that could be responsible for the observed effects. Chemical analysis shows that SE contains hexoses and proteins.
Taken together,S. bryopteris extract may help in stress-induced complications including those due to heat shock. 相似文献