全文获取类型
收费全文 | 2113篇 |
免费 | 147篇 |
国内免费 | 2篇 |
专业分类
2262篇 |
出版年
2022年 | 13篇 |
2021年 | 45篇 |
2020年 | 16篇 |
2019年 | 23篇 |
2018年 | 31篇 |
2017年 | 28篇 |
2016年 | 48篇 |
2015年 | 71篇 |
2014年 | 97篇 |
2013年 | 139篇 |
2012年 | 148篇 |
2011年 | 131篇 |
2010年 | 84篇 |
2009年 | 97篇 |
2008年 | 91篇 |
2007年 | 116篇 |
2006年 | 96篇 |
2005年 | 80篇 |
2004年 | 84篇 |
2003年 | 74篇 |
2002年 | 53篇 |
2001年 | 48篇 |
2000年 | 57篇 |
1999年 | 32篇 |
1998年 | 19篇 |
1997年 | 24篇 |
1996年 | 13篇 |
1995年 | 19篇 |
1994年 | 18篇 |
1993年 | 25篇 |
1992年 | 26篇 |
1991年 | 40篇 |
1990年 | 17篇 |
1989年 | 19篇 |
1988年 | 21篇 |
1987年 | 23篇 |
1986年 | 13篇 |
1985年 | 22篇 |
1984年 | 14篇 |
1983年 | 12篇 |
1979年 | 17篇 |
1977年 | 13篇 |
1976年 | 14篇 |
1975年 | 16篇 |
1974年 | 12篇 |
1973年 | 27篇 |
1972年 | 13篇 |
1971年 | 16篇 |
1967年 | 14篇 |
1966年 | 12篇 |
排序方式: 共有2262条查询结果,搜索用时 15 毫秒
51.
Smith J Sadeyen JR Paton IR Hocking PM Salmon N Fife M Nair V Burt DW Kaiser P 《Journal of virology》2011,85(21):11146-11158
52.
Anandan R Mathew S Sankar TV Viswanathan Nair PG 《Prostaglandins, leukotrienes, and essential fatty acids》2007,76(3):153-158
The protective effect of PUFA concentrate prepared from fish oil on isoproterenol-induced myocardial infarction in male albino rats was investigated with respect to changes in the levels of diagnostic marker enzymes, cholesterol, triglycerides, free fatty acids, phospholipids, reduced glutathione (GSH) and lipid peroxides (LPO). Administration of PUFA concentrate significantly prevented the isoproterenol-induced elevation in the levels of plasma diagnostic marker enzymes (ALT [93.5%], AST [95.6%], LDH [94.7%] and CPK [96.1%]). PUFA concentrate feeding exerted a significant antilipidemic effect against isoproterenol-induced myocardial infarction by reducing the levels of lipid components in plasma (cholesterol [71.5%], triglycerides [79.7%] and free fatty acids [70.7%] and heart tissue (cholesterol [81.4%], triglycerides [76.3%] and free fatty acids [78.6%]). A tendency to prevent the isoproterenol-induced phospholipids depletion (74.4%) in the myocardium of experimental rats was also observed. The level of lipid peroxidation was also found to be significantly lower in PUFA treated animals (2.72+/-0.15nmol/ml in plasma; 1.18+/-0.08nmol/mg protein in heart tissue) as compared to that of isoproterenol-injected groups (5.77+/-0.43nmol/ml in plasma; 2.14+/-0.15nmol/mg protein in heart tissue) of rats. Also the level of reduced GSH significantly higher in the heart tissue of PUFA administered experimental rats (5.65+/-0.98 microg/g) as compared to myocardial infarction induced control rats (2.39+/-0.18 microg/g). The results of the present study indicate that the overall cardioprotective effect of PUFA concentrate is probably related to its ability to inhibit lipid accumulation by its hypolipidaemic property. 相似文献
53.
Enzymatic synthesis of fructose fatty acid ester was performed in organic solvent media, using a purified lipase from Candida antartica B immobilized in acrylic resin. Response surface methodology with a central composite rotatable design based on five levels was implemented to optimize three experimental operating conditions (temperature, agitation and reaction time). A statistical significant cubic model was established. Temperature and reaction time were found to be the most significant parameters. The optimum operational conditions for maximizing the synthesis of fructose esters were 57.1°C, 100 rpm and 37.8 h. The model was validated in the identified optimal conditions to check its adequacy and accuracy, and an experimental esterification percentage of 88.4% (±0.3%) was obtained. These results showed that an improvement of the enzymatic synthesis of fructose esters was obtained under the optimized conditions. 相似文献
54.
Identification of hub proteins from sequence is a challenge in molecular biology. Therefore, it is of interest to predict protein hubs in networks. We describe the prediction of protein "hub" using physiochemical, thermodynamic and conformational properties of amino acid residues in sequence. We have used twenty sequence based features to identify hub behaviour. Linear discriminant analysis and normalised Bayesian approach were utilized for identifying hub proteins solely using these sequence features in E. coli/H. sapiens datasets with accuracies of 99.5/98.6, 87.8/89.6 and 90.1/92.6, respectively. 相似文献
55.
N. MATHAN, M. PARANI, A. PARIDA AND S. NAIR. 1996. Strains of root-nodulating bacteria isolated from Arachis hypogaea showed physiological characteristics of both fast and slow growers. Random amplified polymorphic DNA (RAPD) markers showed most of the genotypes could be identified using one or two primers; however, cluster analysis based on the number of bands shared by the genotypes showed a homogenous cluster. These strains were halotolerant in nature and have potential for use in saline soil. 相似文献
56.
S. D. Jayaraman S. Ismail N. K. Nair V. Navaratnam 《Journal of chromatography. B, Analytical technologies in the biomedical and life sciences》1997,690(1-2):253-257
A method is described for the determination of pyronaridine in plasma using high-performance liquid chromatography with fluorescence detection. The method involves liquid-liquid extraction with phosphate buffer (pH 6.0, 0.05 M) and diethyl ether-hexane (70:30%, v/v) and chromatographic separation on a C18 column (Nucleosil, 250 × 4.6 mm I.D., 5 μm particle size) with acetonitrile-0.05 M phosphate buffer pH 6.0 (60:40%, v/v) as the mobile phase (1 ml/min) and detection by fluorescence (λex = 267 nm, λem = 443 nm). The detector response is linear up to 1000 ng and the overall recoveries pyronaridine and quinine were 90.0 and 60.3%, respectively. The assay procedure was adequately sensitive to measure 10 ng/ml pyronaridine in plasma samples with acceptable precision (< 15% C.V.). The method was found to be suitable for use in clinical pharmacological studies. 相似文献
57.
S. K. Nair C. K. Peethambaran D. Geetha Kamala Nayar K. I. Wilson 《Plant and Soil》1990,125(1):153-154
Solarization of soil was found beneficial for plant growth in cowpea under field conditions. Root nodulation, infection by
mycorrhizal fungi and yield were higher in plants grown in solarized soil. These increases were to the extent of 104.7, 20.0
and 23.7 per cent respectively when compared to control treatment without solarization. 相似文献
58.
Muthu Valliappan Dhaliwal Manpreet Sharma Arunima Nair Divya Kumar H. Mohan Rudramurthy Shivaprakash M. Sehgal Inderpaul Singh Choudhary Hansraj Panda Naresh Chakrabarti Arunaloke Agarwal Ritesh 《Mycopathologia》2022,187(4):355-362
Mycopathologia - In experimental models, the expression of glucose-regulated protein 78 (GRP78) in endothelial cells played a role in the pathogenesis of mucormycosis. However, the role of GRP78 in... 相似文献
59.
Sreejyothi Sankararaman Renjini Ammini Raj Vimal Swapna Mohanachandran Nair Sindhu Sankararaman Sankaranarayana Iyer 《Journal of biological physics》2021,47(2):103-115
Journal of Biological Physics - The paper delves into the plausibility of applying fractal, spectral, and nonlinear time series analyses for lung auscultation. The thirty-five sound signals of... 相似文献
60.
P R Taylor M H Schiffman D Y Jones J Judd A Schatzkin P P Nair R Van Tassell G Block 《Mutation research》1988,206(1):3-9
Correlation studies suggest that fecal mutagenicity is increased in groups eating high-fat diets, the same groups who are often found to have high colorectal cancer incidence and mortality. The fecapentaenes are the best characterized class of fecal mutagens, but the relationship of dietary fat intake to the excretion of these potent genotoxins is unknown. We studied the effect of changes in amount and type of dietary fat on fecapentaene levels in 31 premenopausal women 20-40 years of age who participated in a controlled feeding study. After a pre-diet free-living period lasting 1 menstrual cycle, women were placed on a high-fat (40% energy from fat) diet for 4 menstrual cycles and then switched to a low-fat (20% energy from fat) diet for an additional 4 menstrual cycles. One-half the subjects were maintained throughout the study at a ratio of polyunsaturated-to-saturated fatty acids (P/S ratio) of 1.0, the other half at 0.3; body weight was constant. All meals during the controlled diet periods were prepared at the Human Study Facility of the Beltsville Human Nutrition Research Center. Fecapentaene and fecapentaene precursor levels were measured in acetone extracts from 3-day pooled stool samples collected during the study. No differences in fecapentaene or precursor levels were observed between the high- and low-fat diets at either P/S ratio. Fecapentaene and precursor levels were higher while on controlled diets than during the pre-diet free-living period, and levels declined again in the post-diet free-living period. We conclude that dietary fat has no significant effect on fecapentaene or precursor levels in acetone extracts of stool in premenopausal women. The effect of other dietary or non-dietary factors on fecapentaenes remains unknown. 相似文献