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391.
Chun-Nun Chao Chao-Hung Lai Khan Farheen Badrealam Jeng-Fan Lo Chia-Yao Shen Chia-Hua Chen Ray-Jade Chen Vijaya Padma Viswanadha Wei-Wen Kuo Chih-Yang Huang 《Journal of cellular physiology》2019,234(11):20128-20138
Carboxyl-terminus of Hsc70 interacting protein (CHIP) is a chaperone-dependent E3-ubiquitin ligase with important function in protein quality control system. In the current research endeavor, we have investigated the putative role of CHIP in lipopolysaccharides (LPS)-induced cardiomyopathies. Basically, H9c2 cardiomyoblasts were transfected with CHIP for 24 hr, and thereafter, treated with LPS for 12 hr. Concomitantly, western blot analysis, actin staining, terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay, and coimmunoprecipitation studies were performed to investigate the underlying intricacies. Interestingly, western blot analysis revealed that the expression of hypertrophy and apoptosis-related proteins were considerably reduced following overexpression of CHIP. Moreover, Actin staining and TUNEL assay further ascertained the attenuation of cardiac hypertrophy and apoptosis following overexpression of CHIP respectively. These aspects instigate the role of CHIP in attenuation of LPS-induced cardiomyopathies. Additionally and importantly, co-immunoprecipitation and western blot studies revealed that CHIP plausibly promotes degradation of nuclear factor of activated T cells 3 (NFATc3) through ubiquitin-proteasomal pathway. Taken together, our study reveals that CHIP attenuates LPS-induced cardiac hypertrophy and apoptosis perhaps by promoting NFATc3 proteasomal degradation. 相似文献
392.
393.
Rejection is a common problem after cardiac transplants leading to significant number of adverse events and deaths, particularly in the first year of transplantation. The gold standard to identify rejection is endomyocardial biopsy. This technique is complex, cumbersome and requires a lot of expertise in the correct interpretation of stained biopsy sections. Traditional histopathology cannot be used actively or quickly during cardiac interventions or surgery. Our objective was to develop a stain-less approach using an emerging technology, Fourier transform infrared (FT-IR) spectroscopic imaging to identify different components of cardiac tissue by their chemical and molecular basis aided by computer recognition, rather than by visual examination using optical microscopy. We studied this technique in assessment of cardiac transplant rejection to evaluate efficacy in an example of complex cardiovascular pathology. We recorded data from human cardiac transplant patients’ biopsies, used a Bayesian classification protocol and developed a visualization scheme to observe chemical differences without the need of stains or human supervision. Using receiver operating characteristic curves, we observed probabilities of detection greater than 95% for four out of five histological classes at 10% probability of false alarm at the cellular level while correctly identifying samples with the hallmarks of the immune response in all cases. The efficacy of manual examination can be significantly increased by observing the inherent biochemical changes in tissues, which enables us to achieve greater diagnostic confidence in an automated, label-free manner. We developed a computational pathology system that gives high contrast images and seems superior to traditional staining procedures. This study is a prelude to the development of real time in situ imaging systems, which can assist interventionists and surgeons actively during procedures. 相似文献
394.
395.
Vijaya Parthiban M Michael Gromiha Madenhalli Abhinandan Dietmar Schomburg 《BMC structural biology》2007,7(1):54
Background
Understanding and predicting protein stability upon point mutations has wide-spread importance in molecular biology. Several prediction models have been developed in the past with various algorithms. Statistical potentials are one of the widely used algorithms for the prediction of changes in stability upon point mutations. Although the methods provide flexibility and the capability to develop an accurate and reliable prediction model, it can be achieved only by the right selection of the structural factors and optimization of their parameters for the statistical potentials. In this work, we have selected five atom classification systems and compared their efficiency for the development of amino acid atom potentials. Additionally, torsion angle potentials have been optimized to include the orientation of amino acids in such a way that altered backbone conformation in different secondary structural regions can be included for the prediction model. This study also elaborates the importance of classifying the mutations according to their solvent accessibility and secondary structure specificity. The prediction efficiency has been calculated individually for the mutations in different secondary structural regions and compared. 相似文献396.
Yesudass Antony Prabhu Praveen Kumar Kumar Shanmughavel Piramanayagam Muthu Vijaya Sarathy Samiappan Kavitha 《Bioinformation》2021,17(5):550
It is of interest to document the molecular docking analysis of Cyclin-dependent kinase 1 (CDK-1) inhibitors from Chrysophyllum cainito leaves towards the treatment of tumors using the known structure of PDB ID: 5HQ0. Data shows that molecules such as 8- (Dimethylamino)-7-(3-(4-ethylphenoxy)-2d, ethyl 6-oxo-5-propylheptanoate, 2,3-dihydro-3, 5-dihydroxy-6-methyl-4h-pyran-4-one, 1,2,3-benzenetriol and 1,4-benzenediol 2,5-bis (1,1-dimethylethyl) identified in methanolic extract of C. cainito have binding features with CDK1 for further consideration. 相似文献
397.
Ponnulakshmi Rajagopal Selvaraj Jayaraman Shazia Fathima JH Saravanan Radhakrishnan Patil Ashlesh Laxman Vijaya Prakash Krishnan Muthaiah Satyendra Chandra Tripathi TS Gugapriya Aaditya Madhusudan Tarnekar Gayatri Girish Muthiyan Vishwajit Ravindra Deshmukh Bharat Ramrao Sontakke Kirubhanand Chandrashekar 《Bioinformation》2021,17(11):928
398.
P. Rama Chandra Prasad P. Mamtha Lakshmi K. S. Rajan Vijaya Bhole C. B. S. Dutt 《Biodiversity and Conservation》2012,21(2):309-322
Tropical islands are special and sensitive ecosystems which are subjected to various disturbances imposed by human activities
and natural disasters. A detailed study about the changing landscape scenarios of these fragile island systems induced by
various driving factors could be used for setting up measurements in support of conservation and sustainable development projects.
The current research is a meta-analysis of the studies carried out in Andaman and Nicobar islands which analyzed the impact
of tsunami of 2004 using geospatial tools. Based on the analysis, it was observed that the Nicobar islands were more affected
compared to the Andaman islands. The majority of the researchers used pre- and post-tsunami satellite imagery and adopted
visual interpretation method to delineate the changed classes. The study infers uplift of land in Andaman (exposing) and subsidence
in Nicobar islands (inundation) with severe damage to the coastal elements like mangroves, coral reefs, plantations and in
few cases interior forest. The analysis showed there were no records of the damage for some small islands. Finally, it is
concluded that utility of microwave satellite data for change analysis will prove better in regions like Andaman and Nicobar
where it is difficult to get cloud free optical data because of the high monsoon periods in these islands. It is also suggested
that future work utilizing suitable temporal satellite imagery should focus on the extent of recovery of vegetation and other
coastal elements which suffer the impact of disaster. 相似文献
399.
Boojala V.B. Redely Vijaya Gopal Dipankar Chatterji 《Journal of biomolecular structure & dynamics》2013,31(4):407-419
Abstract The dye Congo red and related self-assembling compounds were found to stabilize immune complexes by binding to antibodies currently engaged in complexation to antigen. In our simulations, it was shown that the site that becomes accessible for binding the supramolecular dye ligand is located in the V domain, and is normally occupied by the N-terminal polypeptide chain fragment. The binding of the ligand disrupts the β-structure in the domain, increasing the plasticity of the antigen-binding site. The higher fluctuation of CDR-bearing loops enhances antigen binding, and allows even low-affinity antibodies to be engaged in immune complexes. Experimental observations of the enhancement effect were supported by theoretical studies using L λ chain (4BJL-PDB identification) and the L chain from the complex of IgM-rheumatoid factor bound to the CH3 domain of the Fc fragment (1ADQ-PDB identification) as the initial structures for theoretical studies of dye-induced changes. Commercial IgM-type rheumatoid factor (human) and sheep red blood cells with coupled IgG (human) were used for experimental tests aimed to reveal the dye- enhancement effect in this system. The specificity of antigen-antibody interaction enhanced by dye binding was studied using rabbit anti-sheep red cell antibodies to agglutinate red cells of different species. Red blood cells of hoofed mammals (horse, goat) showed weak enhancement of agglutination in the presence of Congo red. Neither agglutination nor enhancement were observed in the case of human red cells. The dye-enhancement capability in the SRBC-antiSRBC system was lost after pepsin-digestion of antibodies producing (Fab)2 fragments still agglutinating red cells. Monoclonal (myeloma) IgG, L λ chain and ovoalbumin failed to agglutinate red cells, as expected, and showed no enhancement effect. This indicates that the enhancement effect is specific. 相似文献
400.
Vijaya Kumar T Tiwari AK Robinson A Suresh Babu K Sateesh Chandra Kumar R Anand Kumar D Zehra A Madhusudna Rao J 《Bioorganic & medicinal chemistry letters》2011,21(16):4928-4931
Bergenin (1), major bioactive compound isolated from methanolic extract of Mallotus philippinensis, displayed moderate AGE inhibition activity (IC50 = 186.73 μM). A series of derivatives of bergenin (3a-k) containing variety of aromatic acids were synthesized under mild conditions by modification of sugar part. Selective esterification of hydroxyl groups on the sugar part enhanced antiglycation potential of bergenin. Compounds 3j and 3k exhibited potent antiglycation activity with the IC50 values of 60.75 and 12.28 μM, respectively. 相似文献