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971.
Cláudia S. F. Queiroga Ana S. Almeida Cécile Martel Catherine Brenner Paula M. Alves Helena L. A. Vieira 《The Journal of biological chemistry》2010,285(22):17077-17088
The present work demonstrates the ability of CO to prevent apoptosis in a primary culture of astrocytes. For the first time, the antiapoptotic behavior can be clearly attributed to the inhibition of mitochondrial membrane permeabilization (MMP), a key event in the intrinsic apoptotic pathway. In isolated non-synaptic mitochondria, CO partially inhibits (i) loss of potential, (ii) the opening of a nonspecific pore through the inner membrane, (iii) swelling, and (iv) cytochrome c release, which are induced by calcium, diamide, or atractyloside (a ligand of ANT). CO directly modulates ANT function by enhancing ADP/ATP exchange and prevents its pore-forming activity. Additionally, CO induces reactive oxygen species (ROS) generation, and its prevention by β-carotene decreases CO cytoprotection in intact cells as well as in isolated mitochondria, revealing the key role of ROS. On the other hand, CO induces a slight increase in mitochondrial oxidized glutathione, which is essential for apoptosis modulation by (i) delaying astrocytic apoptosis, (ii) decreasing MMP, and (iii) enhancing ADP/ATP translocation activity of ANT. Moreover, CO and GSSG trigger ANT glutathionylation, a post-translational process regulating protein function in response to redox cellular changes. In conclusion, CO protects astrocytes from apoptosis by preventing MMP, acting on ANT (glutathionylation and inhibition of its pore activity) via a preconditioning-like process mediated by ROS and GSSG. 相似文献
972.
Neide Maria Silva Renato Martins Manzan Cristiane Maria Milanezi Eloísa Amália Vieira Ferro 《Experimental parasitology》2010,126(2):167-9193
In order to investigate the differential ALCAM, ICAM-1 and VCAM-1 adhesion molecules mRNA expression and the blood-brain barrier (BBB) permeability in C57BL/6 and BALB/c mice in Toxoplasma gondii infection, animals were infected with ME-49 strain. It was observed higher ALCAM on day 9 and VCAM-1 expression on days 9 and 14 of infection in the central nervous system (CNS) of C57BL/6 compared to BALB/c mice. The expression of ICAM-1 was high and similar in the CNS of both lineages of infected mice. In addition, C57BL/6 presented higher BBB permeability and higher IFN-γ and iNOS expression in the CNS compared to BALB/c mice. The CNS of C57BL/6 mice presented elevated tissue pathology and parasitism. In conclusion, our data suggest that the higher adhesion molecules expression and higher BBB permeability contributed to the major inflammatory cell infiltration into the CNS of C57BL/6 mice that was not efficient to control the parasite. 相似文献
973.
Many species of leaf‐cutter ants (Atta and Acromyrmex) increase in abundance following natural or anthropogenic disturbances in the vegetation. However, the mechanisms responsible for such an increase are still poorly understood. We evaluated the effects of nesting site and the availability of palatable forage on survival and growth of Atta laevigata colonies at different developmental stages. Foundress queens transplanted into man‐made clearings (dirt roads) had a much higher survival than those transplanted into the adjacent undisturbed savannah vegetation. Similarly, incipient colonies (≥3‐months old) had significantly greater survival and growth rates in dirt roads. In contrast, nesting site did not influence performance of young colonies (≥15‐months old). Both incipient and young colonies responded strongly and positively to experimental supplementation of palatable forage, and this effect was independent of the nesting habitat. Colonies that received extra food grew faster and had a significantly greater survival rate than control colonies. These results suggest that performance of A. laevigata is affected by the generally greater availability of suitable nesting sites and palatable vegetation in disturbed habitats. This may explain how these ants maintain high densities in these habitats, and since the relative importance of these factors changed with colony ontogeny, our findings highlight the importance of evaluating potential limiting factors throughout the full range of an organism's developmental stages. 相似文献
974.
Apoptosis is a major problem in animal cell culture during production of biopharmaceuticals, such as recombinant proteins
or viral particles. In the present work baculovirus-insect cell expression system (BEVS/IC) is used as model to produce rotavirus
like-particles, composed by three layers of three different viral proteins (VP2, VP6 and VP7). In this model baculovirus infection
also induces host cell death. Herein a new strategy to enhance cell life span and to increase recombinant rotavirus protein
production of BEVS/IC system was developed. This strategy relies on hemolymph from Lonomia oblique (total extracts or a semi-purified fraction) medium supplementation. The total extract and a purified fraction from hemolymph
of Lonomia obliqua were able to protect Sf-9 cell culture against apoptosis triggered by oxidative stress (using the pro-oxidant agents tert butylhydroperoxide and hydrogen peroxide) and by baculovirus infection. Furthermore, hemolymph enhance final recombinant
protein production, as it was observed by the increased amounts of VP6 and VP7, which were measured by the semi-quantitative
western blot method. In conclusion, hemolymph medium supplementation can be a promising strategy to improve cell viability
and productivity of recombinant protein in BEVS/IC system. 相似文献
975.
976.
Vasco Manuel Nobre de Carvalho da Silva Vieira Rui Orlando Pimenta Santos 《Journal of phycology》2010,46(6):1128-1137
Studies investigating the demographic traits that drive the patterns of phase dominance (the ploidy ratio) in isomorphic biphasic life cycles have not found an integrative solution. Either fertility or survival has been suggested independently as the main driver. Here, we provide a global theoretical framework on how demographic mechanisms determine the ploidy ratio, unifying previous numerical and observational attempts at this question. The analytical solutions of both the ploidy ratio and its elasticities to model parameters of a stage/size‐structured model patterned after the life cycle of a marine alga were derived and analyzed. A complex interaction among vital rates determines the patterns of phase dominance of biphasic life cycles. Three co‐occurring processes—growth, fertility, and looping—may dominate the dynamics of the population, determining both its growth rate and ploidy ratio. Our analyses show that in species where fertility is low, the ploidy ratio is highly elastic to looping transitions (survival, breakage, and clonal growth). Consequently, the subtle morphological, ecophysiological, and biochemistry phase differences that have been reported in isomorphic life cycles as not explaining the observed ploidy ratios, may, in fact, explain them if they translate into slight phase differences in looping transitions. In species where fertility is low, the looping dissimilarities between phases cannot be too high favoring simultaneously one phase, as the population structure would be completely dominated by that phase. In the case of ecological similarity between phases (equal looping and growth rates between phases), a ploidy ratio different from one can only be set by strong phase differences in fertility. 相似文献
977.
Fernanda Reinhardt Piedras Paulo Roberto Martins Baisch Maria Isabel Corrêa Da Silva Machado Armando Augusto Henriques Vieira Danilo Giroldo 《Journal of phycology》2010,46(3):477-483
This paper describes the influence of nitrate availability on growth and release of dissolved free and combined carbohydrates (DFCHOs and DCCHOs) produced by Spondylosium pygmaeum (Cooke) W. West (Zygnematophyceae). This strain was isolated from a subtropical shallow pond, located at the extreme south of Brazil (Rio Grande, RS). Experiments were carried out in batch culture, comparing two initial nitrate levels (10/100 μM) in the medium. Growth was monitored by direct microscopic cell counts and chl a content. Nitrate consumption was determined by ion chromatography, while the production of extracellular carbohydrates was monitored by the phenol‐sulfuric method. The monosaccharide compositions of DFCHOs and DCCHOs were determined in each growth phase by HPLC with pulse amperometric detection (HPLC‐PAD). At the end of the experiment, the total composition of extracellular polysaccharide (EPS) molecules >12 kDa was determined by gas chromatography. Nitrate availability had no influence on S. pygmaeum cell density at any phase. On the other hand, chl a content decreased after a few days growth when the availability of nitrate was restricted, but continued to rise when nitrate was plentiful. Also, nitrate depletion was faster at 10 μM nitrate. No influence of the growth phase or nitrate availability on the total carbohydrates (TDCHOs) released per cell was observed. Only DCCHOs were released by S. pygmaeum, and the composition varied between growth phases, especially at lower nitrate availability. EPS molecules >12 kDa were composed mainly of xylose, fucose, and galactose, as for other desmids. However, a high N‐acetyl‐glucosamine content was found, uniquely among desmid EPSs. 相似文献
978.
We studied the effect of forest gaps and cattle grazing on survival and growth of seedlings of seven native tree species, planted in dry forest fragments with different intensities of past logging in Central Brazil. Seedling survival after 12 mo ranged from 35 to 77 percent among species. Survival of most species was similar in gaps and understory in minimally disturbed forest remnants, but was lower in the gaps of more heavily logged forests. In contrast, growth was much higher in gaps than in understory in minimally disturbed forests, but for most species was similar in gaps and understory in more heavily logged forests. We did not detect an effect of cattle on seedling survival. Seedlings of the most commonly logged species show a high potential to survive and grow when planted in logged forests. 相似文献
979.
Angela M S Cardonha Regine H S F Vieira Dália P Rodrigues Andrew Macrae Gizele Peirano Grace N D Teophilo 《International microbiology》2004,7(3):213-218
A study on the distribution patterns of enteropathogenic bacteria polluting the shoreline in Natal, Rio Grande do Norte, Brazil, was carried out based on 72 samples obtained from three storm sewers and adjoining beach locations, Praia do Meio (PM), Areia Preta (AP) and Ponta Negra (PN). From each location, 12 water samples were taken and analyzed for fecal coliforms (FC) and Escherichia coli. In AP, two (16.7%) of the seawater samples and five (41.7%) of the storm sewer samples yielded values above 1.1 x 107 FC/100 ml, whereas only one (8.3%) of the samples from PM reached this level. There was no correlation (p > 0.05) between rainfall indices and FC values. A total of 64 E. coli isolates were obtained: 37 from the storm sewer samples and 27 from the seawater samples. Of these isolates, four (O143, two O112ac, and O124) were enteroinvasive and two (O111 and O125) were enteropathogenic. Resistance to antibiotics and to heavy metals was also analyzed. Almost 36% of the E. coli strains isolated were resistant to more than one antibiotic. All strains were resistant to zinc and copper at the highest concentration tested (250 microg/ml), and several (23.4%) were resistant to mercury at 50 microg/ml. Our results agreed with previous reports that antibiotic resistance is commonly associated with heavy-metal resistance in pathogens. 相似文献
980.
The genus Hexapanopeus Rathbun, 1898 contains eight species,of which three are found in the Brazilian coast. The larvaldevelopment of two of these species has already been studiedin the laboratory. This article describes in detail the morphologyof the complete larval stages of Hexapanopeus caribbaeus rearedunder laboratory conditions, and the morphology is comparedwith that of the larvae of the other species of the same genus. 相似文献