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41.
Corinne Vander Wauven Alfred Jann Dieter Haas Thomas Leisinger Victor Stalon 《Archives of microbiology》1988,150(4):400-404
Most Pseudomonas aeruginosa PAO mutants which were unable to utilize l-arginine as the sole carbon and nitrogen source (aru mutants) under aerobic conditions were also affected in l-ornithine utilization. These aru mutants were impaired in one or several enzymes involved in the conversion of N2-succinylornithine to glutamate and succinate, indicating that the latter steps of the arginine succinyltransferase pathway can be used for ornithine catabolism. Addition of aminooxyacetate, an inhibitor of the N2-succinylornithine 5-aminotransferase, to resting cells of P. aeruginosa in ornithine medium led to the accumulation of N2-succinylornithine. In crude extracts of P. aeruginosa an ornithine succinyltransferase (l-ornithine:succinyl-CoA N2-succinyltransferase) activity could be detected. An aru mutant having reduced arginine succinyltransferase activity also had correspondingly low levels of ornithine succinyltransferase. Thus, in P. aeruginosa, these two activities might be due to the same enzyme, which initiates aerobic arginine and ornithine catabolism.Abbreviations OAT
ornithine 5-aminotransferase
- SOAT
N2-succinylornithine 5-aminotransferase
- Oru
ornithine utilization
- Aru
arginine utilization 相似文献
42.
Dr. Teresa I. Mercado Patricia Strickler Kenner C. Rice Victor J. Ferrans 《Current microbiology》1988,16(4):179-183
Studies on the purification of an antitrypanosomal factor (ATF-II) obtained fromPseudomonas fluorescens disclosed that high-pressure liquid chromatography (HPLC) with a Rad-pak porasil (10 silica) column and a GPC-60 Å column was an efficient procedure for the separation of the active components. Extraction of the factor with absolute ethanol prior to elution significantly enhanced the lytic activity of the HPLC eluates, as shown by marked pathologic changes followed by lysis in bioassays performed withTrypanosoma equiperdum. HPLC provided an increase of purification 30 times that obtained with gel filtration of the crude bacterial product. The lipopolysaccharide content of the purified fractions was markedly reduced and indicated an additional advantage for further in vivo tests in experimental infections withT. cruzi. 相似文献
43.
Patch-clamp recordings from ventricular myocytes of neonatal rats identified ionic channels that open in response to membrane stretch caused by negative pressures (1 to 6 cm Hg) in the electrode. The stretch response, consisting of markedly increased channel opening frequency, was maintained, with some variability, during long (>40 seconds) stretch applications. The channels have a conductance averaging 120 pS in isotonic KCl, have a mean reversal potential 31 mV depolarized from resting membrane potential, and do not require external Ca++ for activation. The channels appear to be relatively non-selective for cations. Since they are gated by physiological levels of tension, stretch-activated channels may represent, a cellular control system wherein beat-to-beat tension and/or osmotic balance modulate a portion of membrane conductance.Abbreviations SACs
stretch-activated channels
- HEPES
4-(2-hydroxyethyl)-1-piperazine-ethanesulfonic acid 相似文献
44.
Victor Chude 《Plant and Soil》1988,112(2):293-295
The profile distribution of total and extractable B was determined in 16 Nigerian cacao-growing soil profiles formed from
different parent materials. Total B for all soils ranged from 8 to 54μgg−1 with a mean of 24μgg−1. The soils formed from sandstones in the rainforest zone contained higher amounts of total B than soils derived from basement
complex. Boron extractable in hot water, in 0.1% CaCl2, and in 1N NH4OAc varied from 0.13 to 1.38, 0.44 to 1.20 0.03 to 0.56μgg−1 respectively. The corresponding means were 0.66, 0.75 and 0.27μgg−1 B. Soils on metamorphic rocks gave the highest values. All extractable B values were related to organic matter while only
CaCl2-extractable B correlated with total B. Generally total and extractable B values were higher in the top soils than in the
subsoils. 相似文献
45.
H P Godfrey L S Canfield H L Kindler C V Angadi J J Tomasek J W Goodman 《Journal of immunology (Baltimore, Md. : 1950)》1988,141(5):1508-1515
Azobenzenearsonate-specific cloned mouse T cells able to transfer delayed hypersensitivity reactions in vivo produced macrophage agglutination factor (MaggF) after stimulation with mitogen or antigen in vitro. Mitogen (Con A) elicited MAggF production directly from T cells. Responses to Ag were Ag-specific, required syngeneic accessory cells in addition to T cells, and were independent of T cell fine specificity for azobenzenearsonate. Mouse MAggF shared a number of biochemical and immunochemical properties with the fibronectins (FN): 1) high Mr similar to that of plasma FN; 2) binding to gelatin, heparin, and polyclonal antibodies and mAb specific for cellular and plasma FN; 3) inhibition of activity in solution by monoclonal anti-human FN directed against plasma FN gelatin-binding domain; and 4) action on peritoneal exudate macrophages mediated through a FN-receptor cross reactive with one on human monocytes. MAggF production required active protein synthesis and was associated with significant increases in gelatin-binding immunoreactive FN (Mr 440 kDa on immunoblotting) in culture supernatants and T cell lysates. Metabolically labeled peptides could be precipitated by anti-FN from culture supernatants of activated T cells. Stimulated cultures contained significantly more cells with immunohistologically demonstrable cytoplasmic FN than unstimulated control cultures. We suggest that T cell FN is a distinct species of cellular FN which may play an important role in mediating delayed hypersensitivity inflammatory reactions in vivo. 相似文献
46.
Manickam Sugumaran Steven J. Saul Victor Semensi 《Archives of insect biochemistry and physiology》1988,9(4):269-281
The mechanism of formation of quinone methide from the sclerotizing precursor N-acetyldopamine (NADA) was studied using three different cuticular enzyme systems viz. Sarcophaga bullata larval cuticle, Manduca sexta pharate pupae, and Periplaneta americana presclerotized adult cuticle. All three cuticular samples readily oxidized NADA. During the enzyme-catalyzed oxidation, the majority of NADA oxidized became bound covalently to the cuticle through the side chain with the retention of o-diphenolic function, while a minor amount was recovered as N-acetylnorepinephrine (NANE). Cuticle treated with NADA readily released 2-hydroxy-3′,4′-dihydroxyacetophenone on mild acid hydrolysis confirming the operation of quinone methide sclerotization. Attempts to demonstrate the direct formation of NADA-quinone methide by trapping experiments with N-acetylcysteine surprisingly yielded NADA-quinone-N-acetylcysteine adduct rather than the expected NADA-quinone methide-N-acetylcysteine adduct. These results are indicative of NADA oxidation to NADA-quinone and its subsequent isomerization to NADA-quinone methide. Accordingly, all three cuticular samples exhibited the presence of an isomerase, which catalyzed the conversion of NADA-quinone to NADA-quinone methide as evidenced by the formation of NANE—the water adduct of quinone methide. Thus, in association with phenoloxidase, newly discovered quinone methide isomerase seems to generate quinone methides and provide them for quinone methide sclerotization. 相似文献
47.
Victor N. Mikheev Dmitry S. Pavlov Daria Pakulska 《Environmental Biology of Fishes》1992,35(4):351-360
Synopsis Swimming speed and swimming path of goldfish and tetra larvae were studied in aquaria containing food patches composed of decapsulated cysts and immobilized nauplii of Artemia salina or sparsely distributed prey. The mean swimming speed of starved larvae in the medium without food was about four times higher than the speed of larvae feeding in a patch. Satiated larvae swam about 1.5 times slower than hungry fish. Consumption of single prey items by starved larvae caused the following sequence of swimming responses: handling pause (cessation of swimming), slow swimming in a restricted area, and fast swimming (approximately twice as fast as hungry larvae before encountering food) accompanied by a widening of the area searched (area increased searching). Mean swimming speed was constant over a broad range (101–103 ind·1–1 of food density, although at extreme (high or low) values of food density it depended on swimming responses of the predator. Frequency of visits to the different parts of the aquarium strongly depended on encounters of hungry fish with food particles or patches. 相似文献
48.
In the past year, additional experimental data have expanded our understanding of the molecular mechanisms that underlie nuclear receptor control of regulatory programs. It is increasingly clear -that steroid members (e.g. glucocorticoid and estrogen) and non-steroid members (e.g. retinoic acid, thyroid hormone, and vitamin D) of the nuclear receptor superfamily may utilize distinct strategies in achieving their complex control of gene regulation. 相似文献
49.
In this work, we have calculated the optimal surface area per amphiphile molecule using a two-dimensional gas of electric dipoles interacting with hard The optimal surface area can be evaluated according to a formula (in the two-body approximation) or as a root of an equation (in the three-body approximation). If the equation has more than one positive root, which is greater than the hard core area, then a polydisperse dimensional distribution of supramolecular aggregates is possible. 相似文献
50.
Gary J. Rose James G. Canfield 《Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology》1991,168(4):461-467
Several species of weakly electric fish reflexively change their frequency of electric organ discharge (EOD) in response to sensing signals of similar frequency from conspecifics; that is, they exhibit jamming avoidance responses (JAR).Eigenmannia increases its EOD frequency if jammed by a signal of lower frequency and decreases its EOD frequency if jammed by a signal of higher frequency. This discrimination is based on an analysis of the patterns of amplitude modulations and phase differences resulting from signal interference. Fish of the closely related genus,Sternopygus, however, do not exhibit a JAR. Here we show that despite lacking this behavior,Sternopygus shares many sensory processing capacities withEigenmannia:
相似文献
1. | Fish could be conditioned to discriminate the sign of the frequency difference (Df) between an exogenous sinusoidal signal and its own EODs with as few as 300 training trials. |
2. | Fish can discriminate the sign of Df for jamming signals with an amplitude as low as 2 V/cm (p-p); which is approximately 40 dB below the amplitude of the fish's EOD, as measured lateral to the operculum. |
3. | Fish appear to discriminate the sign of Df by evaluating modulations in signal amplitude and differences in the timing of signals received by different areas of the body surface. |
4. | Sternopygus processes electrosensory information through band-pass modulation filters. Tuning to modulation rates over at least 4–16 Hz exists. |