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21.
In higher plant cells, there are some enzymes capable of utilizing pyrophosphate (PPi ) as an energy donor. Among these, membrane-bound proton pumping pyrophosphatases (H+ -PPi ase) have been identified. In addition to the well-known vacuolar H+ -PPi ase (V-PPi ase), there is evidence for the presence of a mitochondrial H+ -PPi ase. This enzyme is localized on the inner surface of the inner membrane and catalyzes the specific hydrolysis of PPi , coupled to proton transport, with a H+ /PPi stoichiometry of ca 2. This activity is Mg2+ -requiring, is stimulated by monovalent cations, and is inhibited by Ca2+ , F− and diphosphonates. The H+ -PPi ase contains a catalytic head which is constituted by a 35-kDa protein which is loosely bound to the inner membrane. This protein exhibits a PPi ase activity, stimulated by phospholipids, with characteristics very similar to the membrane-bound enzyme. The mitochondrial PPi ase is distinct from the V-PPi ase, because an antibody raised against the 35-kDa protein does not react with tonoplast membranes. The mitochondrial H+ -PPi ase seems to have an F-type structure, similar to the F-ATP synthase and the membrane-bound PPi ases from mammalian and yeast mitochondria. It is suggested that, beside synthesizing PPi , this enzyme may act as a buffer for the electrochemical proton gradient, by hydrolyzing PPi, during conditions of oxygen deprivation. 相似文献
22.
Marco Zancani Carlo Peresson Antonino Biroccio Giorgio Federici Andrea Urbani Irene Murgia Carlo Soave Fulvio Micali Angelo Vianello Francesco Macrì 《European journal of biochemistry》2004,271(18):3657-3664
In this work, evidence for the presence of ferritins in plant mitochondria is supplied. Mitochondria were isolated from etiolated pea stems and Arabidopsis thaliana cell cultures. The proteins were separated by SDS/PAGE. A protein, with an apparent molecular mass of approximately 25-26 kDa (corresponding to that of ferritin), was cross-reacted with an antibody raised against pea seed ferritin. The mitochondrial ferritin from pea stems was also purified by immunoprecipitation. The purified protein was analyzed by MALDI-TOF mass spectrometry and the results of both mass finger print and peptide fragmentation by post source decay assign the polypeptide sequence to the pea ferritin (P < 0.05). The mitochondrial localization of ferritin was also confirmed by immunocytochemistry experiments on isolated mitochondria and cross-sections of pea stem cells. The possible role of ferritin in oxidative stress of plant mitochondria is discussed. 相似文献
23.
M Rossetto F Vianello A Rigo U Vrhovsek F Mattivi M Scarpa 《Free radical research》2001,35(6):933-939
A stable ESR signal, centred at g = 2.0037 +/- 0.0002, characterised by a single resonance and assignable to a free radical, was found in all the bottled red wines, both commercial and experimental, that we have examined. The radical concentration was calculated to be in the range of 5-82 nM. After exposure of the wines to air for a few minutes a two fold increase of the ESR signal, followed by a slow decrease with time, was observed. The intensity of ESR signal in experimental red wines, was found to increase with the ageing of the wines and was strictly correlated to the total content of polyphenols. The formation of semiquinone radicals of polyphenols is suggested as one possible mechanism leading to the presence of stable free radicals in red wines. 相似文献
24.
Braidot E Petrussa E Bertolini A Peresson C Ermacora P Loi N Terdoslavich M Passamonti S Macrì F Vianello A 《Planta》2008,228(1):203-213
During maturation, Vitis vinifera berries accumulate a large amount of several anthocyanins in the epidermal tissue, whereas their precursors and intermediates are ubiquitously synthesized within the fruit. Up to date, several mechanisms of flavonoid transport at subcellular level have been hypothesized, but it is not possible to identify a general model applicable in every plant tissue and organ. Recently, a putative anthocyanin carrier, homologue to mammalian bilitranslocase (BTL) (TC 2.A.65.1.1), was found in Dianthus caryophyllus petal microsomes. In the present paper, an immunohistochemical and immunochemical analysis, using an antibody raised against a BTL epitope, evidences the expression and function of such a transporter in V. vinifera berries (cv. Merlot). Specific localisations of the putative carrier within berry tissues together with expression changes during different developmental stages are shown. Water stress induces an increase in protein expression in both skin and pulp samples. A bromosulfalein (BSP) uptake activity, inhibitable by the BTL antibody, is detected in berry mesocarp microsomes, with K (m) = 2.39 microM BSP and V (max) = 0.29 micromol BSP min(-1) mg(-1) protein. This BSP uptake is also competitively inhibited by quercetin (K (i) = 4 microM). A putative role for this carrier is discussed in relation to the membrane transport of secondary metabolites. 相似文献
25.
Tram Huynh Zhong Chen Suhong Pang Jieping Geng Tiziano Bandiera Simona Bindi Paola Vianello Fulvia Roletto Sandrine Thieffine Arturo Galvani Wayne Vaccaro Michael A. Poss George L. Trainor Matthew V. Lorenzi Marco Gottardis Lata Jayaraman Ashok V. Purandare 《Bioorganic & medicinal chemistry letters》2009,19(11):2924-2927
Design, synthesis, and SAR development led to the identification of the potent, novel, and selective pyrazole based inhibitor (7f) of Coactivator Associated Arginine Methyltransferase (CARM1). 相似文献
26.
Pevarello P Fancelli D Vulpetti A Amici R Villa M Pittalà V Vianello P Cameron A Ciomei M Mercurio C Bischoff JR Roletto F Varasi M Brasca MG 《Bioorganic & medicinal chemistry letters》2006,16(4):1084-1090
We have recently reported about a new class of Aurora-A inhibitors based on a bicyclic tetrahydropyrrolo[3,4-c]pyrazole scaffold. Here we describe the synthesis and early expansion of CDK2/cyclin A-E inhibitors belonging to the same chemical class. Synthesis of the compounds was accomplished using a solution-phase protocol amenable to rapid parallel expansion. Compounds with nanomolar activity in the biochemical assay and able to efficiently inhibit CDK2-mediated tumor cell proliferation have been obtained. 相似文献
27.
Soares TN Melo DB Resende LV Vianello RP Chaves LJ Collevatti RG Telles MP 《American journal of botany》2012,99(2):e72-e73
? Premise of the study: Microsatellite markers were developed for the population genetic analyses of the neotropical tree Dipteryx alata (Fabaceae). ? Methods and Results: Microsatellites were developed from a genomic shotgun library. Polymorphism at each microsatellite loci was analyzed based on 94 individuals from three populations. Eight loci amplified successfully and presented one to 10 alleles, and expected heterozygosities ranged from 0.097 to 0.862. Four loci also amplified in Pterodon emarginatus and presented similar polymorphism. ? Conclusion: The eight microsatellite primer pairs are potentially suitable for population genetic studies and successfully amplified in another Fabaceae species. 相似文献
28.
The generation of H2O2 by isolated pea stem mitochondria, oxidizing either malate plus glutamate or succinate, was examined. The level of H2O2 was almost one order of magnitude higher when mitochondria were energized by succinate. The succinate-dependent H2O2 formation was abolished by malonate, but unaffected by rotenone. The lack of effect of the latter suggests that pea mitochondria were working with a proton motive force below the threshold value required for reverse electron transfer. The activation by pyruvate of the alternative oxidase was reflected in an inhibition of H2O2 formation. This effect was stronger when pea mitochondria oxidized malate plus glutamate. Succinate-dependent H2O2 formation was ca. four times lower in Arum sp. mitochondria (known to have a high alternative oxidase) than in pea mitochondria. An uncoupler (FCCP) completely prevented succinate-dependent H2O2 generation, while it only partially (40-50%) inhibited that linked to malate plus glutamate. ADP plus inorganic phosphate (transition from state 4 to state 3) also inhibited the succinate-dependent H2O2 formation. Conversely, that dependent on malate plus glutamate oxidation was unaffected by low and stimulated by high concentrations of ADP. These results show that the main bulk of H2O2 is formed during substrate oxidation at the level of complex II and that this generation may be prevented by either dissipation of the electrochemical proton gradient (uncoupling and transition state 4-state 3), or preventing its formation (alternative oxidase). Conversely, H2O2 production, dependent on oxidation of complex I substrate, is mainly lowered by the activation of the alternative oxidase. 相似文献
29.
T. Bui Minh H. Bauer A. T. Eprintsev M. A. Pinheiro de Carvalho M. Eriksson R. Gardeström G. Samuelsson P. Gardeström T. K. Golovko B. I. Gulyaev A. A. Igamberdiev V. N. Popov M. I. Falaleeva E. Labedzka Z. Sadoch M. Hernet T. Panczyk H. Lambers U. Lernmark N. S. Mamushina E. K. Zubkova O. V. Voitsekhovskaya M. Mikulska J-L. Bomsel A. M. Rychter T. Pobezhimova V. Voinikov N. Varakina G. Borovsky T. N. Popova A. U. Igamberdiev Yu. I. Velichko I. Scheurwater C. Cornelissen G. F. Tudorake P. V. Negru O. L. Cerbu A. Vianello E. Petrussa F. Macri A. M. Wagner M. J. Wagner M. Zancani F. Macri A. Dal Belin Peruffo A. Vianello 《Biologia Plantarum》1994,36(1):S175-S184
30.
Radish (Raphanus sativus L.) microsomal vesicles show a vanadate-?nd nitrate-insensitive, and imidodiphosphate-sensitive electrogenictransport of protons dependent upon addition of inorganic pyrophosphate(PP) or ADP. The activity is detectable in preparations from24 h-old seedlings and increases about 3 fold in vesicles from72 h-old seedlings. The ADP-dependent proton uptake, being preventedby inorganic pyrophosphatase, used as a PP scavenging system,can be ascribed to enzymes utilizing ADP and producing PP whichappears the only substrate for the proton pumping PPase. TheH+-PPase has a Km of ca. 10 µM for the translocating functionand 20 µM for the hydrolytic activity. It has a pH optimumnear to 7.0 and is stimulated by certain monovalent cations(K+, Rb+ and Cs+). The majority of this activity is associatedwith a high density (3545% sucrose interface) fractionwhich is enriched for vanadate-sensitive, nitrate-insensitiveATPase activity. (Received September 11, 1989; Accepted December 22, 1989) 相似文献