全文获取类型
收费全文 | 491篇 |
免费 | 23篇 |
专业分类
514篇 |
出版年
2024年 | 1篇 |
2023年 | 9篇 |
2022年 | 13篇 |
2021年 | 16篇 |
2020年 | 13篇 |
2019年 | 7篇 |
2018年 | 24篇 |
2017年 | 19篇 |
2016年 | 24篇 |
2015年 | 29篇 |
2014年 | 31篇 |
2013年 | 37篇 |
2012年 | 37篇 |
2011年 | 38篇 |
2010年 | 18篇 |
2009年 | 24篇 |
2008年 | 26篇 |
2007年 | 20篇 |
2006年 | 11篇 |
2005年 | 15篇 |
2004年 | 23篇 |
2003年 | 13篇 |
2002年 | 13篇 |
2001年 | 9篇 |
2000年 | 9篇 |
1999年 | 2篇 |
1998年 | 8篇 |
1997年 | 2篇 |
1996年 | 1篇 |
1995年 | 1篇 |
1994年 | 1篇 |
1993年 | 2篇 |
1992年 | 2篇 |
1990年 | 3篇 |
1989年 | 1篇 |
1985年 | 1篇 |
1983年 | 1篇 |
1982年 | 1篇 |
1980年 | 1篇 |
1979年 | 2篇 |
1977年 | 3篇 |
1974年 | 1篇 |
1972年 | 1篇 |
1970年 | 1篇 |
排序方式: 共有514条查询结果,搜索用时 0 毫秒
41.
Sobreira Rodrigues D Avila de Carvalho H Almeida Fernandes A Freitas CM Cerqueira Leite R de Oliveira PR 《Memórias do Instituto Oswaldo Cruz》2002,97(6):853-856
The ixodid Amblyomma aureolatum is suspected to play a role in the epidemiology of wild life-cycle hemoparasites, which frequently infect dogs in rural and hunting areas in Brazil. Little is known about its bionomics. The objective of the present study was to evaluate some bionomic aspects of A. aureolatum ticks in Brazil. One engorged female, collected from a dog (Canis familiaris) in S?o Sebasti?o das Aguas Claras, State of Minas Gerais, was used to establish a colony in the laboratory. Subsequently its parasitic stage progeny were fed on domestic dogs and laboratory animals. The free-living stages were incubated at 27 degrees C +/- 2 degrees C and minimum 70% relative humidity in a BOD incubator. The egg incubation period ranged from 31 to 34 days; the parasitic period of larvae ranged from 4 to 6 days and ecdysis to nymphs occurred from day 19 up to day 22. The parasitic period of nymphs ranged from 5 to 8 days and the period of ecdysis to adults from 31 to 33 days. The parasitic period of adults ranged from 11 to 15 days, the pre-oviposition period from 6 to 12 days, and the oviposition period from 9 to 38 days. The total duration of the life cycle ranged from 116 to 168 days. 相似文献
42.
Barbisan LF Scolastici C Miyamoto M Salvadori DM Ribeiro LR da Eira AF de Camargo JL 《Genetics and molecular research : GMR》2003,2(3):295-308
The effects of crude extracts of the mushroom Agaricus blazei Murrill (Agaricaceae) on both DNA damage and placental form glutathione S-transferase (GST-P)-positive liver foci induced by diethylnitrosamine (DEN) were investigated. Six groups of adult male Wistar rats were used. For two weeks, animals of groups 3 to 6 were treated with three aqueous solutions of A. blazei (mean dry weight of solids being 1.2, 5.6, 11.5 and 11.5 mg/ml, respectively). After this period, groups 2 to 5 were given a single ip injection 200 mg/kg DEN and groups 1 and 6 were treated with 0.9% NaCl. All animals were subjected to 70% partial hepatectomy at week five and sacrificed 4, 24 and 48 h or 8 weeks after DEN or 0.9% NaCl treatments (10th week after the beginning of the experiment). The alkaline comet assay and GST-P-positive liver foci development were used to evaluate the influence of the mushroom extracts on liver cell DNA damage and on the initiation of liver carcinogenesis, respectively. Previous treatment with the highest concentration of A. blazei (11.5 mg/ml) significantly reduced DNA damage, indicating a protective effect against DEN-induced liver cytotoxicity/genotoxicity. However, the same dose of mushroom extract significantly increased the number of GST-P-positive liver foci. 相似文献
43.
Costa-Lotufo LV Jimenez PC Wilke DV Leal LK Cunha GM Silveira ER Canuto KM Viana GS Moraes ME de Moraes MO Pessoa C 《Zeitschrift für Naturforschung. C, Journal of biosciences》2003,58(9-10):675-680
Amburana cearensis a common tree found in Northeastern Brazil is widely used in folk medicine. The present work evaluated the cytotoxicity of kaempferol, isokaempferide, amburoside A and protocatechuic acid isolated from the ethanol extract of the trunk bark of A. cearensis. The compounds were tested for their cytotoxicity on the sea urchin egg development, hemolysis assay and 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide assay using tumor cell lines. Isokaempferide and kaempferol, but not amburoside A and protocatechuic acid, inhibited the sea urchin egg development as well as tumor cell lines, but in this assay isokaempferide was more potent than kaempferol. Protocatechuic acid was the only compound able to induce hemolysis of mouse erythrocytes, suggesting that the cytotoxicity of kaempferol and isokaempeferide was not related to membrane damage. 相似文献
44.
Possible biphasic sweating response during short-term heat acclimation protocol for tropical natives
Magalhães Fde C Machado-Moreira CA Vimieiro-Gomes AC Silami-Garcia E Lima NR Rodrigues LO 《Journal of physiological anthropology》2006,25(3):215-219
The aim of the present study was to evaluate the sweat loss response during short-term heat acclimation in tropical natives. Six healthy young male subjects, inhabitants of a tropical region, were heat acclimated by means of nine days of one-hour heat-exercise treatments (40+/-0 degrees C and 32+/-1% relative humidity; 50% (.)VO(2peak) on a cycle ergometer). On days 1 to 9 of heat acclimation whole-body sweat loss was calculated by body weight variation corrected for body surface area. On days 1 and 9 rectal temperature (T(re)) and heart rate (HR) were measured continuously, and rating of perceived exertion (RPE) every 4 minutes. Heat acclimation was confirmed by reduced HR (day 1 rest: 77+/-5 b.min(-1); day 9 rest: 68+/-3 b.min(-1); day 1 final exercise: 161+/-15 b.min(-1); day 9 final exercise: 145+/-11 b.min(-1), p<0.05), RPE (13 vs. 11, p<0.05) and T(re) (day 1 rest: 37.2+/-0.2 degrees C; day 9 rest: 37.0+/-0.2 degrees C; day 1 final exercise: 38.2+/-0.2 degrees C; day 9 final exercise: 37.9+/-0.1 degrees C, p<0.05). The main finding was that whole-body sweat loss increased in days 5 and 7 (9.49+/-1.84 and 9.56+/-1.86 g.m(-2).min(-1), respectively) compared to day 1 (8.31+/-1.31 g.m(-2).min(-1), p<0.05) and was not different in day 9 (8.48+/-1.02 g.m(-2).min(-1)) compared to day 1 (p>0.05) of the protocol. These findings are consistent with the heat acclimation induced adaptations and suggest a biphasic sweat response (an increase in the sweat rate in the middle of the protocol followed by return to initial values by the end of it) during short-term heat acclimation in tropical natives. 相似文献
45.
Rodríguez-Díaz J Montava R Viana R Buesa J Pérez-Martínez G Monedero V 《Biotechnology letters》2011,33(6):1169-1175
The efficacy of recombinant Lactococcus lactis as a delivery vehicle for a rotavirus antigen was evaluated in a mouse model. The rotavirus VP8* protein was expressed intracellularly
and extracellularly in L. lactis wild type and in an alr mutant deficient in alanine racemase activity, necessary for the synthesis of the cell-wall component d-alanine. When the mucosal immune response was evaluated by measuring VP8*-specific IgA antibody in faeces, wild-type L. lactis triggered a low IgA synthesis only when the secreting strain was used. In contrast, VP8*-specific IgA was detected in faeces
of both groups of mice orally given the alr mutant expressing extracellular VP8* and intracellular VP8*, which reached levels similar to that obtained with the wild
type secreting strain. However, oral administration of the recombinant strains did not induce serum IgG or IgA responses.
L. lactis cell-wall mutants may therefore provide certain advantages when low-antigenic proteins are expressed intracellularly. However,
the low immune response obtained by using this antigen-bacterial host combination prompts to the use of new strains and vaccination
protocols in order to develop acceptable rotavirus immunization levels. 相似文献
46.
47.
Alexandre Costa Adilson Luís Pereira Silva Rommel Bezerra Viana Auro Atsushi Tanaka Jaldyr de Jesus Gomes VarelaJr 《Journal of molecular modeling》2016,22(9):217
Theoretical chemistry calculations using the Density Functional Theory (DFT) were carried out to understand the interaction between oxygen (O2) and MnN4 type manganese-based complexes during the formation of MnN4-O2 adducts. In order to understand how this interaction is affected by different macrocyclic ligands, O2 was bonded to manganese-porphyrin (MnP), manganese-octamethylporphyrin (MnOMP), manganese-tetraaza[14]annulene (MnTAA), manganese-dibenzo [b,i] [1, 4, 8, 11]-tetraaza [14] annulene (MnDBTAA), manganese-2,3,9,10-tetramethyl-1,4,8,11-tetraazacyclotetradeca-1,3,8,10-tetraene ([(tim)Mn]2+), and manganese-2,3,9,10-tetraphenyl-1,4,8,11-tetraazacyclotetradeca-1,3,8,10-tetraene ([(ph-tim)Mn]2+). The binding and activation of the oxygen molecule was facilitated by an increasing trend in the O-O bond lengths and a decreasing one in the O-O vibrational frequency, with preference for the O2 side-on interaction among MnN4 macrocycles. The catalytic activities of the MnN4 complexes toward the O2 binding process increased in the following order: [(ph-tim)Mn]2+?<?MnP?<?MnOMP?<?MnDBTAA?<?MnTAA?<?[(tim)Mn]2+. Therefore, it was concluded that the [(tim)Mn]2+complex was the most active for the binding and activation of molecular oxygen. 相似文献
48.
Effects of storage were tested on germination ofDioscorea composita (Dioscoreaceae) seeds. Freshly collected seeds and seeds stored at 25°C in paper bags from 1 to 11 mo or for 4 and 5 yr were used in most of the experiments. Seeds were tested for germination at 20, 25, 30, 35, 25–20, and 25–35°C in white light and in darkness. Initiation of germination was delayed in freshly harvested seeds, and dormancy was reduced in seeds stored for about 9 mo. Viability of the seeds decreased after 4 and 5 yr of storage. 相似文献
49.
50.
T. Raz Tim Barrett Raymonde Szargel Hanna Mandel Ellis J. Neufeld Kazuto Nosaka Marcos B. Viana N. Cohen 《Human genetics》1998,103(4):455-461
Thiamine-responsive megaloblastic anemia (TRMA, also known as Rogers syndrome, OMIM 249270) is a rare autosomal recessive
disorder characterized by a triad of megaloblastic anemia, diabetes mellitus, and sensorineural deafness. Patients respond,
to varying degrees, to treatment with megadoses of thiamine. We have recently shown genetic linkage of the TRMA gene to a
16-centimorgan (cM) region on 1q23.2–1q23.3 based on the analysis of four large, inbred families of Alaskan, Italian, and
Israeli-Arab origin. Here we narrow the TRMA interval down to 4 cM based on genetic recombination, homozygosity mapping, and
linkage disequilibrium (highest LOD score of 12.5 at D1S2799, at a recombination fraction of 0). We provide further evidence that the TRMA gene is located in this region and confirm
the homogeneity of the disease. In this analysis, we genotyped seven additional families of diverse ethnic origin (Pakistani,
Indian, Italian, Brazilian, and Japanese), and analyzed additional markers in two previously reported families showing evidence
of linkage disequilibrium in a large area of their haplotypes. The multi-system manifestations of TRMA suggest that thiamine
has a pivotal role in a multiplicity of physiological processes. Mapping the TRMA gene and understanding the molecular basis
of the disease might, thus, shed light on the role of thiamine in common disorders such as deafness, anemia, and diabetes.
Received: 16 April 1998 / Accepted: 6 July 1998 相似文献