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91.
The present study intended to verify which factors (phylogenetic relationship or local ecological conditions) would determine how Frieseomelitta bees use floral resources. The data obtained in the literature on biocenotic studies of Apoidea visiting flowers in Brazil were analyzed, with identification and quantification of floral resources used by Frieseomelitta species in different areas. The phenogram of similarity on use of resources among Frieseomelitta species was compared to the phylogenetic hypothesis proposed for the group. Among the eight Frieseomelitta species registered in 19 studies, F. doederleini (Friese), F. francoi (Moure), F. languida Moure, F. varia (Lepeletier) e Frieseomelitta sp. nov., were collected using resources from 36 plant families. F. doederleini, F. languida e F. varia centralized their activity in Caesalpiniaceae, Malpighiaceae e Anacardiaceae. The similarities and disparities found among areas and Frieseomelitta species reflected the similarities and differences of the vegetation composition in each area. Frieseomelitta bees presented a forage pattern similar to that one presented by other highly social bees, visiting flowers of many plant species, but concentrating their activities in few plant species. Despite the low phylogenetic relationship between F. languida and F. doederleini, they showed a high similarity on the use of floral resource, while F. varia e F. languida, species with high phylogenetic relationship, showed low similarity in the use of resource. Although the data obtained are not conclusive, it indicates that phylogenetic restrictions do not influence the pattern of use of floral resource by Frieseomelitta bees. 相似文献
92.
Campos Prmula Viana Schaefer Carlos Ernesto G. R. Pontara Vanessa Xavier Mrcio Vencius Barbosa do Vale Jnior Jos Frutuoso Corra Guilherme Resende Villa Pedro Manuel 《Evolutionary ecology》2022,36(1):55-73
Evolutionary Ecology - Understanding how environmental drivers induce changes in plant composition and diversity across evolutionary time can provide important insights into community assembly... 相似文献
93.
Eric?Roberto?Guimar?es?Rocha Aguiar Roenick Proveti Olmo Simona Paro Flavia Viana Ferreira Isaque?Jo?o?da?Silva de?Faria Yaovi?Mathias?Honore Todjro Francisco Pereira Lobo Erna Geessien Kroon Carine Meignin Derek Gatherer Jean-Luc Imler Jo?o Trindade Marques 《Nucleic acids research》2015,43(13):6191-6206
Virus surveillance in vector insects is potentially of great benefit to public health. Large-scale sequencing of small and long RNAs has previously been used to detect viruses, but without any formal comparison of different strategies. Furthermore, the identification of viral sequences largely depends on similarity searches against reference databases. Here, we developed a sequence-independent strategy based on virus-derived small RNAs produced by the host response, such as the RNA interference pathway. In insects, we compared sequences of small and long RNAs, demonstrating that viral sequences are enriched in the small RNA fraction. We also noted that the small RNA size profile is a unique signature for each virus and can be used to identify novel viral sequences without known relatives in reference databases. Using this strategy, we characterized six novel viruses in the viromes of laboratory fruit flies and wild populations of two insect vectors: mosquitoes and sandflies. We also show that the small RNA profile could be used to infer viral tropism for ovaries among other aspects of virus biology. Additionally, our results suggest that virus detection utilizing small RNAs can also be applied to vertebrates, although not as efficiently as to plants and insects. 相似文献
94.
Fonseca JF Bruschi JH Santos IC Viana JH Magalhães AC 《Animal reproduction science》2005,85(1-2):117-124
The objective of this study was to evaluate two protocols of estrous synchronization in non-lactating Toggenburg goats. Nineteen goats were allocated, according to body condition score and weight, into two groups (A and B) and evaluated utilizing two treatments (T1 and T2). Animals in the T1 and T2 groups received an intravaginal sponge (day 0) containing 60 mg medroxyprogesterone acetate for 6 and 9 days, respectively, plus 200 IU equine chorionic gonadotrophin (eCG) and 22.5 microg cloprostenol 24 h before sponge removal. Females were bred only at the second estrus and received 22.5 microg cloprostenol 7 days later to prevent pregnancy. Percentages of animals in estrus did not differ (P > 0.05) between T1 (89.5%) and T2 (84.2%). From 33 females in estrus (T1 + T2), 28 (84.8%), 2 (6.1%), and 3 (9.1%) were identified in estrus at 06:00, 12:00 and 18:00 h, respectively. Additionally, 6 (18.2%), 0 (0.0%) and 27 (81.8%) were no longer detected to be on estrus at 06:00, 12:00 and 18:00 h, respectively. Interval from sponge removal and the onset of estrus (IE) did not differ (P > 0.05) between T1 (46.1 +/- 15.0 h) and T2 (53.6 +/- 16.1 h). Duration of estrus did not differ (P > 0.05) between T1 (30.0 +/- 12.0 h) and T2 (27.2 +/- 11.2 h). Both protocols were effective in inducing estrus in non-lactating goats. The onset and end of the estrus relative to hour of the day should be considered in estrous detection, natural breeding, and artificial insemination in goats. 相似文献
95.
Background
It has been argued that multibreed animal models should include a heterogeneous covariance structure. However, the estimation of the (co)variance components is not an easy task, because these parameters can not be factored out from the inverse of the additive genetic covariance matrix. An alternative model, based on the decomposition of the genetic covariance matrix by source of variability, provides a much simpler formulation. In this study, we formalize the equivalence between this alternative model and the one derived from the quantitative genetic theory. Further, we extend the model to include maternal effects and, in order to estimate the (co)variance components, we describe a hierarchical Bayes implementation. Finally, we implement the model to weaning weight data from an Angus × Hereford crossbred experiment.Methods
Our argument is based on redefining the vectors of breeding values by breed origin such that they do not include individuals with null contributions. Next, we define matrices that retrieve the null-row and the null-column pattern and, by means of appropriate algebraic operations, we demonstrate the equivalence. The extension to include maternal effects and the estimation of the (co)variance components through the hierarchical Bayes analysis are then straightforward. A FORTRAN 90 Gibbs sampler was specifically programmed and executed to estimate the (co)variance components of the Angus × Hereford population.Results
In general, genetic (co)variance components showed marginal posterior densities with a high degree of symmetry, except for the segregation components. Angus and Hereford breeds contributed with 50.26% and 41.73% of the total direct additive variance, and with 23.59% and 59.65% of the total maternal additive variance. In turn, the contribution of the segregation variance was not significant in either case, which suggests that the allelic frequencies in the two parental breeds were similar.Conclusion
The multibreed maternal animal model introduced in this study simplifies the problem of estimating (co)variance components in the framework of a hierarchical Bayes analysis. Using this approach, we obtained for the first time estimates of the full set of genetic (co)variance components. It would be interesting to assess the performance of the procedure with field data, especially when interbreed information is limited. 相似文献96.
Viana T Loureiro-Dias MC Loureiro V Prista C 《Applied and environmental microbiology》2012,78(17):6302-6308
Intracellular pH (pH(in)) is a tightly regulated physiological parameter, which controls cell performance in all living systems. The purpose of this work was to evaluate if and how H(+) homeostasis is accomplished by an industrial wine strain of Saccharomyces cerevisiae while fermenting real must under the harsh winery conditions prevalent in the late stages of the fermentation process, in particular low pH and high ethanol concentrations and temperature. Cells grown at 15, 25, and 30°C were harvested in exponential and early and late stationary phases. Intracellular pH remained in the range of 6.0 to 6.4, decreasing significantly only by the end of glucose fermentation, in particular at lower temperatures (pH(in) 5.2 at 15°C), although the cells remained viable and metabolically active. The cell capability of extruding H(+) via H(+)-ATPase and of keeping H(+) out by means of an impermeable membrane were evaluated as potential mechanisms of H(+) homeostasis. At 30°C, H(+) efflux was higher in all stages. The most striking observation was that cells in late stationary phase became almost impermeable to H(+). Even when these cells were challenged with high ethanol concentrations (up to 20%) added in the assay, their permeability to H(+) remained very low, being almost undetectable at 15°C. Comparatively, ethanol significantly increased the H(+) permeability of cells in exponential phase. Understanding the molecular and physiological events underlying yeast H(+) homeostasis at late stages of fermentations may contribute to the development of more robust strains suitable to efficiently produce a high-quality wine. 相似文献
97.
98.
Pontes B Viana NB Campanati L Farina M Neto VM Nussenzveig HM 《European biophysics journal : EBJ》2008,37(2):121-129
We investigate properties of a reported new mechanism for cell–cell interactions, tunneling nanotubes (TNT’s). TNT’s mediate
actin-based transfer of vesicles and organelles and they allow signal transmission between cells. The effects of lateral pulling
with polystyrene beads trapped by optical tweezers on TNT’s linking separate U-87 MG human glioblastoma cells in culture are
described. This cell line was chosen for handling ease and possible pathology implications of TNT persistence in communication
between cancerous cells. Observed nanotubes are shown to have the characteristic features of TNT’s. We find that pulling induces
two different types of TNT bifurcations. In one of them, termed V-Y bifurcation, the TNT is first distorted into a V-shaped
form, following which a new branch emerges from the apex. In the other one, termed I-D bifurcation, the pulled TNT is bent
into a curved arc of increasingly broader span. Curves showing the variation of pulling force with displacement are obtained.
Results yield information on TNT structure and elastic properties.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
99.
Lery LM von Krüger WM Viana FC Teixeira KR Bisch PM 《Biochimica et biophysica acta》2008,1784(11):1578-1589
A proteomic view of G. diazotrophicus PAL5 at the exponential (E) and stationary phases (S) of cultures in the presence of low (L) and high levels (H) of combined nitrogen is presented. The proteomes analyzed on 2D-gels showed 131 proteins (42E+32S+29H+28L) differentially expressed by G. diazotrophicus, from which 46 were identified by combining mass spectrometry and bioinformatics tools. Proteins related to cofactor, energy and DNA metabolisms and cytoplasmic pH homeostasis were differentially expressed in E growth phase, under L and H conditions, in line with the high metabolic rate of the cells and the low pH of the media. Proteins most abundant in S-phase cells were stress associated and transporters plus transferases in agreement with the general phenomenon that binding protein-dependent systems are induced under nutrient limitation as part of hunger response. Cells grown in L condition produced nitrogen-fixation accessory proteins with roles in biosynthesis and stabilization of the nitrogenase complex plus proteins for protection of the nitrogenases from O(2)-induced inactivation. Proteins of the cell wall biogenesis apparatus were also expressed under nitrogen limitation and might function in the reshaping of the nitrogen-fixing G. diazotrophicus cells previously described. Genes whose protein products were detected in our analysis were mapped onto the chromosome and, based on the tendency of functionally related bacterial genes to cluster, we identified genes of particular pathways that could be organized in operons and are co-regulated. These results showed the great potential of proteomics to describe events in G. diazotrophicus cells by looking at proteins expressed under distinct growth conditions. 相似文献