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41.
42.
L Chrobák D Radochová K Smetana F Mat?ja M Kout J Polák P Dít? J Pribrosky I Dvorácková 《Folia haematologica (Leipzig, Germany : 1928)》1980,107(4):628-640
These siblings of a Czech family aged 21, 19 and 6 years, respectively, with congenital dyserythropoietic anemia, type II, (HEMPAS) are reported. In two elder siblings ferrokinetic studies revealed a rapid plasma 59Fe clearance, markedly decreased erythrocyte incorporation and shortened 51Cr red-cell survival. Direct anti-globulin test was found positive in one of them. Further investigations revealed low values of blood plasma cholesterol, total lipids, beta-lipoproteins, beta-carotine and vitamin E and A as well as low values of the prothrombin complex. Liver biopsy demonstrated siderosis and disseminated intravascular coagulation in the liver in both patients. The possible reasons for these humoral aberrations are discussed. 相似文献
43.
Petronela Weisová Ujval Anilkumar Caitriona Ryan Caoimhín G. Concannon Jochen H.M. Prehn Manus W. Ward 《BBA》2012,1817(5):744-753
The preconditioning response conferred by a mild uncoupling of the mitochondrial membrane potential (Δψm) has been attributed to altered reactive oxygen species (ROS) production and mitochondrial Ca2 + uptake within the cells. Here we have explored if altered cellular energetics in response to a mild mitochondrial uncoupling stimulus may also contribute to the protection. The addition of 100 nM FCCP for 30 min to cerebellar granule neurons (CGNs) induced a transient depolarization of the Δψm, that was sufficient to significantly reduce CGN vulnerability to the excitotoxic stimulus, glutamate. On investigation, the mild mitochondrial ‘uncoupling’ stimulus resulted in a significant increase in the plasma membrane levels of the glucose transporter isoform 3, with a hyperpolarisation of Δψm and increased cellular ATP levels also evident following the washout of FCCP. Furthermore, the phosphorylation state of AMP-activated protein kinase (AMPK) (Thr 172) was increased within 5 min of the uncoupling stimulus and elevated up to 1 h after washout. Significantly, the physiological changes and protection evident after the mild uncoupling stimulus were lost in CGNs when AMPK activity was inhibited. This study identifies an additional mechanism through which protection is mediated upon mild mitochondrial uncoupling: it implicates increased AMPK signalling and an adaptive shift in energy metabolism as mediators of the preconditioning response associated with FCCP-induced mild mitochondrial uncoupling. 相似文献
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Z. Ouanes I. Ayed-Boussema T. Baati E.E. Creppy H. Bacha 《Mutation Research - Genetic Toxicology and Environmental Mutagenesis》2005,565(2):139-149
The cytogenetic effect of zearalenone (ZEN), a non-steroidal estrogenic mycotoxin, was evaluated in vivo, in mouse bone marrow cells, by assessing the percentage of cells bearing different chromosome aberrations. The studies included different conditions for animal treatment, as follows: (1) single intraperitoneal (ip) injection, (2) repeated ip injections, (3) pre-treatment for 24 h with Vitamin E (Vit E), and (4) pre-treatment for 4 h with 17β-estradiol (17β-Est) or progesterone (Prog). ZEN induced different types of chromosome aberrations, which was concentration-dependent (2–20 mg/kg bw). These doses corresponded to 0.4–4% of the LD50 in the mouse. Interestingly, when the dose of ZEN (40 mg/kg) was fractionated into four equivalent doses (4 × 10 mg/kg bw), into three doses (15 + 10 + 15 mg/kg bw), or into two equivalent doses (2 × 20 mg/kg bw), given every 24 h, the percentage of chromosome aberrations increased significantly. This finding suggests that ZEN proceeds by reversible binding on receptors that could become saturated, and that it damages the chromosomes in a ‘hit and go’ manner. Furthermore, pre-treatment of animals with 17β-estradiol or progesterone significantly decreased the percentage of chromosome aberrations, suggesting that (i) these hormones bind to the same cytoplasmic receptors transported into the nucleus to elicit DNA damage, (ii) they may play a role in preventing chromosome aberrations induced by ZEN. Similarly, Vit E prevented these chromosome aberrations indicating that Vit E, previously reported to prevent most of the toxic effects induced by ZEN, may also bind to the same receptors. 相似文献
47.
F Krizsa Z Borbényi E Arokszállásy I Cserháti 《Folia haematologica (Leipzig, Germany : 1928)》1980,107(4):683-687
The thrombopoietic serum activity was examined in rats during thrombocytopenia produced by bleeding or after treatment with antithrombocyte serum (ATS). 6 hours after both treatments the thrombopoietic activity of the serum, i.e. its content of thrombopoietin, is increased. After the ATS treatment of nephrectomized animals a similar increase of thrombopoietic activity as in normal animals could be achieved. In contrast to that, no similar increase of thrombopoietic activity was observed in nephrectomized animals after blood loss. According to the results of the authors the increase of thrombopoietic activity produced by different stimuli can be attributed to different mechanisms. 相似文献
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The role of arctic zooplankton in biogeochemical cycles: respiration and excretion of ammonia and phosphate during summer 总被引:1,自引:0,他引:1
M. Alcaraz R. Almeda A. Calbet E. Saiz C. M. Duarte S. Lasternas S. Agustí R. Santiago J. Movilla A. Alonso 《Polar Biology》2010,33(12):1719-1731
The study of the structural and functional properties of key components of polar marine ecosystems has received increased
attention in order to better understand the ecological consequences of future sea temperature rise and seasonal ice retraction.
Owing to this purpose, during the ATOS-Arctic cruise, held in July 2007 in the framework of the 2007–2008 International Polar
Year, we studied the respiratory carbon demand of mesozooplankton as well as their contribution to the regeneration of inorganic
nitrogen and phosphorus (NH4-N and PO4-P) via excretion. The studied area comprised several stations along a latitudinal gradient in the East Greenland current, plus a
network of stations NW of the Svalbard islands. The specific respiratory carbon losses and phosphorus (PO4-P) excretion rates were similar or slightly higher than some reports for Arctic mesozooplankton, but the nitrogen (NH4-N) excretion rates were higher by a factor of 3 when compared with previous data sets. The mesozooplankton respiratory losses
were equivalent to 23% of primary production, and at turn zooplankton contributed by excretion to more than 50% of the N and
P required by phytoplankton. Although C:N, C:P and N:P metabolic atomic quotients almost coincided with the average Redfield’s
stoichiometric ratios, the low C:N values when compared to previous reports suggested a predominance of protein-related metabolic
substrates. The potential consequences of changes observed in the C:N, N:P and C:P metabolic ratios of mesozooplankton for
Arctic marine ecosystems are discussed. 相似文献
50.
A recent literature review of commentaries and ‘state of the art’ articles from researchers in psychiatric genetics (PMG)
offers a consensus about progress in the science of genetics, disappointments in the discovery of new and effective treatments,
and a general optimism about the future of the field. I argue that optimism for the field of psychiatric molecular genetics
(PMG) is overwrought, and consider progress in the field in reference to a sample estimate of US National Institute of Mental
Health funding for this paradigm for the years 2008 and 2009. I conclude that the amounts of financial investment in PMG is
questionable from an ethical perspective, given other research and clinical needs in the USA. 相似文献