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排序方式: 共有221条查询结果,搜索用时 109 毫秒
81.
Loch S Klauschies F Schölz C Verweij MC Wiertz EJ Koch J Tampé R 《The Journal of biological chemistry》2008,283(19):13428-13436
Cytotoxic T lymphocytes eliminate infected cells upon surface display of antigenic peptides on major histocompatibility complex I molecules. To promote immune evasion, UL49.5 of several varicelloviruses interferes with the pathway of major histocompatibility complex I antigen processing. However, the inhibition mechanism has not been elucidated yet. Within the macromolecular peptide-loading complex we identified the transporter associated with antigen processing (TAP1 and TAP2) as the prime target of UL49.5. Moreover, we determined the active oligomeric state and crucial elements of the viral factor. Remarkably, the last two residues of the cytosolic tail of UL49.5 are essential for endoplasmic reticulum (ER)-associated proteasomal degradation of TAP. However, this process strictly requires additional signaling of an upstream regulatory element in the ER lumenal domain of UL49.5. Within this new immune evasion mechanism, we show for the first time that additive elements of a small viral factor and their signaling across the ER membrane are essential for targeted degradation of a multi-subunit membrane complex. 相似文献
82.
Verweij W Spelt C Di Sansebastiano GP Vermeer J Reale L Ferranti F Koes R Quattrocchio F 《Nature cell biology》2008,10(12):1456-1462
83.
Peter de Bruijn Diederik F. S. Kehrer Jaap Verweij Alex Sparreboom 《Journal of chromatography. B, Analytical technologies in the biomedical and life sciences》2001,753(2):395-400
A high-performance liquid chromatographic assay with UV detection has been developed for the determination of ketoconazole in human plasma. Quantitative extraction was achieved by a single solvent extraction involving a mixture of acetonitrile–n-butyl chloride (1:4, v/v). Ketoconazole and the internal standard (clotrimazole) were separated on a column packed with Inertsil ODS-80A material and a mobile phase composed of water–acetonitrile–tetrahydrofuran–ammonium hydroxide–triethylamine (45:50.2:2.5:0.1:0.1, v/v). The column effluent was monitored at a wavelength of 206 nm with a detector range set at 0.5. The calibration graph was linear in the range of 20–2000 ng/ml, with a lower limit of quantitation of 20.0 ng/ml. The extraction recoveries for ketoconazole and clotrimazole in human plasma were 93±9.7% and 83±10.0%, respectively. The developed method has been successfully applied to a clinical study to examine the pharmacokinetics of ketoconazole in a cancer patient. 相似文献
84.
Construction of cDNA coding for human von Willebrand factor using antibody probes for colony-screening and mapping of the chromosomal gene. 总被引:10,自引:2,他引:10 下载免费PDF全文
C L Verweij C J de Vries B Distel A J van Zonneveld A G van Kessel J A van Mourik H Pannekoek 《Nucleic acids research》1985,13(13):4699-4717
Von Willebrand Factor (vWF) mRNA was identified in fractionated polyA+ RNA preparations isolated from cultured human endothelial cells. Micro-injection of specific polyA+ RNA fractions in Xenopus laevis oocytes provoked the synthesis of a vWF-like product which could be detected with an immunoradiometric assay relying on Sepharose-linked monoclonal anti-vWF IgG and different radiolabeled monoclonal anti-vWF IgGs. A vWF-mRNA-containing polyA+ RNA preparation served as substrate for a size-selected cDNA-expression library of 60 000 colonies which was screened for the synthesis of antigens related to vWF, using polyclonal anti-vWF IgG and a second antibody conjugated with peroxidase. Eight positive colonies were detected of which two reacted strongly in the enzyme-linked assay. Immunoblotting of bacterial extracts of "expression clones" with a monoclonal anti-vWF IgG revealed polypeptides which size fits within the length of the cDNA insertions. Northern blotting of human endothelial RNA, employing fragments of vWF cDNA as probes, showed specific hybridization with a mRNA of about 9000 nucleotides. DNA-sequence analysis of a vWF-cDNA insertion revealed an open reading frame followed by a translation stopcodon. It is argued that the cDNA insertions encode the carboxy-terminal part of the vWF protein. vWF-cDNA probes were employed to map the von Willebrand factor gene on chromosome 12 using a panel of 35 human-rodent somatic cell hybrids. 相似文献
85.
Abstract. Dry alvar grasslands on limestone on the Baltic island of Öland, SE Sweden, are very species-rich as long as the traditional agricultural exploitation of grazing and fire wood collection continues. After abandonment, encroachment of Juniperus communis starts and a closed woodland can develop within 100 yr. A chronosequence, representing a successional series, was used for the comparison of sites still grazed, and sites ungrazed for about 20, 55 and 80 yr, respectively. Out of the 58 characteristic dry alvar grassland species 55 % disappeared from the established vegetation after 80 yr of abandoning, and 80 % also vanished from the seed bank. Arenaria serpyllifolia, Trifolium repens, Agrostis vinealis, Linum catharticum, Polygala vulgaris, Cerastium fontanum, Luzula campestris, Achillea millefolium and Potentilla tabernaemontani were the only species left in the seed bank. More than 75 % of the dry alvar grassland species were classified as having a transient or short-term persistent seed bank. It is concluded that restoration management, by cutting junipers, of overgrown dry alvar grassland cannot rely on the longevity of seeds in the soil. Seeds have to be dispersed by wind or grazing animals. 相似文献
86.
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88.
The regulatory role of protons in hyphal tip growth was investigated by using membrane-permeant weak acids to acidify cytoplasm of the oomycete Saprolegnia ferax. Acetic acid decreased cytoplasmic pH from approximately pH 7.2 to 6.8, as shown by SNARF-1 measurements of cytoplasmic pH. Inhibition of growth in a dose-dependent manner by acetic, propionic, and isobutyric acid was accompanied by changes in positioning and morphology of mitochondria and nuclei, condensation of chromatin, disruptions in peripheral actin, and increases in hyphal diameter. These cellular alterations were fully reversible, and during recovery, major cytoplasmic movements and extensive apical vacuolations were observed. The results are consistent with proton regulation of the cytoskeleton, nuclear matrix, and/or chromosomes. However, a macroscopic cytoplasmic gradient of H+ in hyphae was not revealed by SNARF-1, indicating that if such a H+ gradient were required, it must occur at a finer level than we detected. 相似文献
89.
W. J. Loos J. Verweij K. Nooter G. Stoter A. Sparreboom 《Journal of chromatography. B, Analytical technologies in the biomedical and life sciences》1997,693(2):1471
A sensitive reversed-phase high-performance liquid chromatographic method has been developed and validated for the quantitative determination of docetaxel (I) in human plasma. The concentrations in plasma, for validation procedures spiked with known amounts of I, are read from calibration curves in the range of 10–20 000 ng/ml. The sample preparation involved a liquid–liquid extraction of 1000 μl of sample with a mixture of acetonitrile–n-butylchloride (1:4, v/v). The related compound paclitaxel (II) was used as internal standard. Chromatographic separations were performed an Inertsil ODS-80A column, with UV detection performed at 230 nm. The overall extraction recoveries were 84.3 and 90.0% for I and II, respectively. The lower limit of quantitation was 10 ng/ml, and the accuracy, within-run and between-run precisions at three tested concentrations fell within the generally accepted criteria for bioanalytical assays. 相似文献
90.
A GFP-MAP4 reporter gene for visualizing cortical microtubule rearrangements in living epidermal cells 总被引:15,自引:7,他引:8 下载免费PDF全文
J Marc CL Granger J Brincat DD Fisher Th Kao AG McCubbin RJ Cyr 《The Plant cell》1998,10(11):1927-1940
Microtubules influence morphogenesis by forming distinct geometrical arrays in the cell cortex, which in turn affect the deposition of cellulose microfibrils. Although many chemical and physical factors affect microtubule orientation, it is unclear how cortical microtubules in elongating cells maintain their ordered transverse arrays and how they reorganize into new geometries. To visualize these reorientations in living cells, we constructed a microtubule reporter gene by fusing the microtubule binding domain of the mammalian microtubule-associated protein 4 (MAP4) gene with the green fluorescent protein (GFP) gene, and transient expression of the recombinant protein in epidermal cells of fava bean was induced. The reporter protein decorates microtubules in vivo and binds to microtubules in vitro. Confocal microscopy and time-course analysis of labeled cortical arrays along the outer epidermal wall revealed the lengthening, shortening, and movement of microtubules; localized microtubule reorientations; and global microtubule reorganizations. The global microtubule orientation in some cells fluctuates about the transverse axis and may be a result of a cyclic self-correcting mechanism to maintain a net transverse orientation during cellular elongation. 相似文献