排序方式: 共有102条查询结果,搜索用时 15 毫秒
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Mateos-Langerak J Brink MC Luijsterburg MS van der Kraan I van Driel R Verschure PJ 《Molecular biology of the cell》2007,18(4):1464-1471
The heterochromatin protein 1 (HP1) family is thought to be an important structural component of heterochromatin. HP1 proteins bind via their chromodomain to nucleosomes methylated at lysine 9 of histone H3 (H3K9me). To investigate the role of HP1 in maintaining heterochromatin structure, we used a dominant negative approach by expressing truncated HP1alpha or HP1beta proteins lacking a functional chromodomain. Expression of these truncated HP1 proteins individually or in combination resulted in a strong reduction of the accumulation of HP1alpha, HP1beta, and HP1gamma in pericentromeric heterochromatin domains in mouse 3T3 fibroblasts. The expression levels of HP1 did not change. The apparent displacement of HP1alpha, HP1beta, and HP1gamma from pericentromeric heterochromatin did not result in visible changes in the structure of pericentromeric heterochromatin domains, as visualized by DAPI staining and immunofluorescent labeling of H3K9me. Our results show that the accumulation of HP1alpha, HP1beta, and HP1gamma at pericentromeric heterochromatin domains is not required to maintain DAPI-stained pericentromeric heterochromatin domains and the methylated state of histone H3 at lysine 9 in such heterochromatin domains. 相似文献
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Xavier Bailly Elisa Giuntini M Connor Sexton Ryan PJ Lower Peter W Harrison Nitin Kumar J Peter W Young 《The ISME journal》2011,5(11):1722-1734
We investigated the genomic diversity of a local population of the symbiotic bacterium Sinorhizobium medicae, isolated from the roots of wild Medicago lupulina plants, in order to assess genomic diversity, to identify genomic regions influenced by duplication, deletion or strong selection, and to explore the composition of the pan-genome. Partial genome sequences of 12 isolates were obtained by Roche 454 shotgun sequencing (average 5.3 Mb per isolate) and compared with the published sequence of S. medicae WSM 419. Homologous recombination appears to have less impact on the polymorphism patterns of the chromosome than on the chromid pSMED01 and megaplasmid pSMED02. Moreover, pSMED02 is a hot spot of insertions and deletions. The whole chromosome is characterized by low sequence polymorphism, consistent with the high density of housekeeping genes. Similarly, the level of polymorphism of symbiosis genes (low) and of genes involved in polysaccharide synthesis (high) may reflect different selection. Finally, some isolates carry genes that may confer adaptations that S. medicae WSM 419 lacks, including homologues of genes encoding rhizobitoxine synthesis, iron uptake, response to autoinducer-2, and synthesis of distinct polysaccharides. The presence or absence of these genes was confirmed by PCR in each of these 12 isolates and a further 27 isolates from the same population. All isolates had rhizobitoxine genes, while the other genes were co-distributed, suggesting that they may be on the same mobile element. These results are discussed in relation to the ecology of Medicago symbionts and in the perspective of population genomics studies. 相似文献
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ProADD, a database for protein aggregation diseases, is developed to organize the data under a single platform to facilitate easy
access for researchers. Diseases caused due to protein aggregation and the proteins involved in each of these diseases are
integrated. The database helps in classification of proteins involved in the protein aggregation diseases based on sequence and
structural analysis. Analysis of proteins can be done to mine patterns prevailing among the aggregating proteins.
Availability
http://bicmku.in/ProADD 相似文献69.
Martijn S. Luijsterburg Christoffel Dinant Hannes Lans Jan Stap Elzbieta Wiernasz Saskia Lagerwerf Dani?l O. Warmerdam Michael Lindh Maartje C. Brink Jurek W. Dobrucki Jacob A. Aten Maria I. Fousteri Gert Jansen Nico P. Dantuma Wim Vermeulen Leon H.F. Mullenders Adriaan B. Houtsmuller Pernette J. Verschure Roel van Driel 《The Journal of cell biology》2009,185(4):577-586
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David M Markusic Niek P van Til Johan K Hiralall PJ Oude Ronald Elferink Jurgen Seppen 《BMC biotechnology》2009,9(1):85