A Field Study to Investigate Environmental Factors that Could Effect Microcystin Synthesis of a Microcystis Population in the Bautzen Reservoir

Toxicity of Microcystis blooms to warm-blooded animals generated by microcystins has bew reported world wide. The ecological relevance of microcystin production for cyanobacteria remains unknown. The microcystin concentration in Microcystis blooms occurring in the Bautzen reservoir Was investigated. The microcystin content of samples were determined by HPLC and ranged from undetectabel to 14.7 μg mg⁻¹. Various chemical and physical parameters were monitored at the same time as Microcystis sampling, however, there was no correlation between these parameters and microcystin dynamics. The spatial distribution of microcystin in the Microcystis population was investigated once and showed no difference between samples taken at five stations. The microcystin concentration in ihc cell free water from the reservoir was below the detection threshold (< 1 μg L⁻¹). Size dependent Fractions of the Microcystis population analyzed for microcystin concentration correlated with colony sim. In the small fraction (>30 <66 μm) microcystin was not detected. In the medium fraction (> 6 h < 100μm) lower microcystin concentrations were detected than in large fraction (>100μm) in which the highest microcystin concentrations were found.     

Synthesis of monooxime-monocarbamoyl bispyridinium compounds bearing (E)-but-2-ene linker and evaluation of their reactivation activity against tabun- and paraoxon-inhibited acetylcholinesterase

Six AChE monooxime-monocarbamoyl reactivators with an (E)-but-2-ene linker were synthesized using modification of currently known synthetic pathways. Their potency to reactivate AChE inhibited by the nerve agent tabun and insecticide paraoxon was tested in vitro. The reactivation efficacies of pralidoxime, HI-6, obidoxime, K048, K075 and the newly prepared reactivators were compared. According to the results obtained, one reactivator seems to be promising against tabun-inhibited AChE and two reactivators against paraoxon-inhibited AChE. The best results were obtained for bisquaternary substances with at least one oxime group in position four.     

DNA-free recombinant SV40 capsids protect mice from acute renal failure by inducing stress response, survival pathway and apoptotic arrest

Viruses induce signaling and host defense during infection. Employing these natural trigger mechanisms to combat organ or tissue failure is hampered by harmful effects of most viruses. Here we demonstrate that SV40 empty capsids (Virus Like Particles-VLPs), with no DNA, induce host Hsp/c70 and Akt-1 survival pathways, key players in cellular survival mechanisms. We postulated that this signaling might protect against organ damage in vivo. Acute kidney injury (AKI) was chosen as target. AKI is critical, prevalent disorder in humans, caused by nephrotoxic agents, sepsis or ischemia, via apoptosis/necrosis of renal tubular cells, with high morbidity and mortality. Systemic administration of VLPs activated Akt-1 and upregulated Hsp/c70 in vivo. Experiments in mercury-induced AKI mouse model demonstrated that apoptosis, oxidative stress and toxic renal failure were significantly attenuated by pretreatment with capsids prior to the mercury insult. Survival rate increased from 12% to >60%, with wide dose response. This study demonstrates that SV40 VLPs, devoid of DNA, may potentially be used as prophylactic agent for AKI. We anticipate that these finding may be projected to a wide range of organ failure, using empty capsids of SV40 as well as other viruses.     

Characterization of the AGR2 Interactome Uncovers New Players of Protein Disulfide Isomerase Network in Cancer Cells

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H protein of bacteriophage 16-3 and RkpM protein of Sinorhizobium meliloti 41 are involved in phage adsorption

The strain-specific capsular polysaccharide KR5 antigen of Sinorhizobium meliloti 41 is required both for invasion of the symbiotic nodule and for the adsorption of bacteriophage 16-3. In order to know more about the genes involved in these events, bacterial mutants carrying an altered phage receptor were identified by using host range phage mutants. A representative mutation was localized in the rkpM gene by complementation and DNA sequence analysis. A host range phage mutant isolated on these phage-resistant bacteria was used to identify the h gene, which is likely to encode the tail fiber protein of phage 16-3. The nucleotide sequences of the h gene as well as a host range mutant allele were also established. In both the bacterial and phage mutant alleles, a missense mutation was found, indicating a direct contact between the RkpM and H proteins in the course of phage adsorption. Some mutations could not be localized in these genes, suggesting that additional components are also important for bacteriophage receptor recognition.     

Respiration of wood ant nest material affected by material and forest stand characteristics

We studied differences in respiration of materials from different parts of wood ant nest (top, bottom, and rim) and from the nest surroundings (humus layer and mineral soil). Samples were taken from 8 wood ant (Formica aquilonia) nests in each of the two types of forest (birch and pine) in eastern Finland. The differences were related to material and forest stand characteristics (i.e., moisture, pH, carbon content, and C:N ratio). As a result, the highest respiration per g DW was measured at the top of ant nests in the birch forest. However, respiration did not significantly differ between the parts of ant nests in the pine forest. Respiration of the humus layers in both forest stands was on average higher, whereas respiration of the mineral soils in both forest stands was lower in comparison with respiration of the nest materials. The respiration per g C did not show any significant differences between different parts of nests and surrounding soil. The most important factors influencing respiration of the materials appeared to be moisture, carbon content, and pH. In conclusion, respiration of wood ant nest material is affected by the specific material and forest stand characteristics.     

Statistical vs. Stochastic experimental design: An experimental comparison on the example of protein refolding

Optimization of experimental problems is a challenging task in both engineering and science. In principle, two different design of experiments (DOE) strategies exist: statistical and stochastic methods. Both aim to efficiently and precisely identify optimal solutions inside the problem‐specific search space. Here, we evaluate and compare both strategies on the same experimental problem, the optimization of the refolding conditions of the lipase from Thermomyces lanuginosus with 26 variables under study. Protein refolding is one of the main bottlenecks in the process development for recombinant proteins. Despite intensive effort, the prediction of refolding from sequence information alone is still not applicable today. Instead, suitable refolding conditions are typically derived empirically in large screening experiments. Thus, protein refolding should constitute a good performance test for DOE strategies. We compared an iterative stochastic optimization applying a genetic algorithm and a standard statistical design consisting of a D‐optimal screening step followed by an optimization via response surface methodology. Our results revealed that only the stochastic optimization was able to identify optimal refolding conditions (～1.400 U g^?1 refolded activity), which were 3.4‐fold higher than the standard. Additionally, the stochastic optimization proved quite robust, as three independent optimizations performed similar. In contrast, the statistical DOE resulted in a suboptimal solution and failed to identify comparable activities. Interactions between process variables proved to be pivotal for this optimization. Hence, the linear screening model was not able to identify the most important process variables correctly. Thereby, this study highlighted the limits of the classic two‐step statistical DOE. © 2012 American Institute of Chemical Engineers Biotechnol. Prog., 2012