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71.
Prabha Sarangi Roland Steinacher Veronika Altmannova Qiong Fu Tanya T. Paull Lumir Krejci Matthew C. Whitby Xiaolan Zhao 《PLoS genetics》2015,11(1)
Protein modifications regulate both DNA repair levels and pathway choice. How each modification achieves regulatory effects and how different modifications collaborate with each other are important questions to be answered. Here, we show that sumoylation regulates double-strand break repair partly by modifying the end resection factor Sae2. This modification is conserved from yeast to humans, and is induced by DNA damage. We mapped the sumoylation site of Sae2 to a single lysine in its self-association domain. Abolishing Sae2 sumoylation by mutating this lysine to arginine impaired Sae2 function in the processing and repair of multiple types of DNA breaks. We found that Sae2 sumoylation occurs independently of its phosphorylation, and the two modifications act in synergy to increase soluble forms of Sae2. We also provide evidence that sumoylation of the Sae2-binding nuclease, the Mre11-Rad50-Xrs2 complex, further increases end resection. These findings reveal a novel role for sumoylation in DNA repair by regulating the solubility of an end resection factor. They also show that collaboration between different modifications and among multiple substrates leads to a stronger biological effect. 相似文献
72.
Manfred E. Beutel Sabine Fischbeck Harald Binder Maria Blettner Elmar Br?hler Katharina Emrich Peter Friedrich-Mai Barbara H. Imruck Veronika Weyer Sylke R. Zeissig 《PloS one》2015,10(1)
Aim
The purpose of the study was to determine anxiety and depression, quality of life, and their determinants in long-term survivors of malignant melanoma.Methods
In a state cancer registry a cohort of survivors of malignant melanoma was contacted via the physician registered. Of 1302 contactable patients, 689 (52.2%) completed a questionnaire including the Patient Health Questionnaire with generalized anxiety (GAD-7) and depression (PHQ-9) and the EORTC Quality of Life Questionnaire (EORTC QLQ 30). Based on multiple regression analysis, predictors of quality of life and distress were identified. Comparison data were assessed in two waves of representative face-to-face household surveys of the adult German population.Results
An average of 8.4 (5.7 to 12.2) years after diagnosis, distress was higher in women compared to men and in middle adulthood (vs. older patients). Symptoms were higher in women than in men, and there was a decline of functioning and increase of symptoms across the age range of both genders. Compared to the general population, there were slightly increased depression and anxiety (only women), but no impaired global quality of life. Yet, survivors evidenced functional decline and more physical symptoms. Distress and reduced quality of life were consistently predicted by lack of social support, fear of recurrence, pessimism and self-blame. Distress was increased by a family history of melanoma, and additional mental and somatic diseases.Conclusion
Overall, long-term survivors have adjusted well achieving a global quality of life comparable to the general population. Yet, compromised functional dimensions, physical symptoms and distress indicate the need for integrating psychooncological screening into oncological follow-up, which might be guided by predictors such as family history or social support. Further prospective study is needed to determine the course of adaptation to the disease and corroborate the risk factors identified. 相似文献73.
Tereza Krejcova Miroslava Smelcova Jaroslav Petr Jean-Francois Bodart Marketa Sedmikova Jan Nevoral Marketa Dvorakova Alena Vyskocilova Ivona Weingartova Veronika Kucerova-Chrpova Eva Chmelikova Lenka Tumova Frantisek Jilek 《PloS one》2015,10(1)
Porcine oocytes that have matured in in vitro conditions undergo the process of aging during prolonged cultivation, which is manifested by spontaneous parthenogenetic activation, lysis or fragmentation of aged oocytes. This study focused on the role of hydrogen sulfide (H2S) in the process of porcine oocyte aging. H2S is a gaseous signaling molecule and is produced endogenously by the enzymes cystathionine-β-synthase (CBS), cystathionine-γ-lyase (CSE) and 3-mercaptopyruvate sulfurtransferase (MPST). We demonstrated that H2S-producing enzymes are active in porcine oocytes and that a statistically significant decline in endogenous H2S production occurs during the first day of aging. Inhibition of these enzymes accelerates signs of aging in oocytes and significantly increases the ratio of fragmented oocytes. The presence of exogenous H2S from a donor (Na2S.9H2O) significantly suppressed the manifestations of aging, reversed the effects of inhibitors and resulted in the complete suppression of oocyte fragmentation. Cultivation of aging oocytes in the presence of H2S donor positively affected their subsequent embryonic development following parthenogenetic activation. Although no unambiguous effects of exogenous H2S on MPF and MAPK activities were detected and the intracellular mechanism underlying H2S activity remains unclear, our study clearly demonstrates the role of H2S in the regulation of porcine oocyte aging. 相似文献
74.
Objective
In this systematic review, we estimate the prevalence of six types of arthritis in Africa; namely rheumatoid arthritis, osteoarthritis, juvenile arthritis, psoriatic arthritis, gout, and ankylosing spondylitis.Methods
We comprehensively searched literature on 31 August 2014 in MEDLINE, EMBASE, Web of Science and the Cochrane Library to identify eligible studies from 1975 up to 31 July 2014. Two review authors independently selected studies, extracted data, and appraised studies. We carried out random effects meta-analysis of prevalence of arthritis and assessed heterogeneity through subgroup analyses. We performed separate analyses for population- and hospital-based studies, as well as rural and urban settings.Main Findings
We included 27 cross-sectional studies (20 population-based and 7 hospital-based) from Africa reporting on the prevalence of arthritis. The majority of the studies were from South Africa (44.4%, 12/27). Rheumatoid arthritis in urban settings ranged from 0.1% in Algeria, 0.6% in the DRC, to a meta-analysis overall prevalence of 2.5% in South Africa, and in rural settings ranged from a meta-analysis overall prevalence of 0.07% in South Africa, 0.3% in Egypt, to 0.4% in Lesotho. Osteoarthritis was the most prevalent form of arthritis and in urban settings it was 55.1% in South Africa and in rural settings, all in South Africa, ranged from 29.5%, 29.7%, up to 82.7% among adults aged over 65 years. Other results include highest prevalence of 33.1% for knee osteoarthritis in rural South Africa, 0.1% for ankylosing spondylitis in rural South Africa, 4.4% for psoriatic arthritis in urban South Africa, 0.7% for gout in urban South Africa, and 0.3% for juvenile idiopathic arthritis in urban Egypt. A third of the included studies had a low risk of bias (33.3%, 9/27), 40.8% (11/27) moderate risk, and 25.9% (7/27) had a high risk of bias.Conclusions
In this systematic review, we have identified the paucity of latest prevalence data on arthritis in Africa. More studies are needed to address the prevalence and the true burden of this disease in Africa. 相似文献75.
76.
Jakub Kreisinger Pavel Munclinger Veronika Javůrková Tomáš Albrecht 《Journal of avian biology》2010,41(5):551-557
A method that was based on non‐invasive sampling of genetic material was used to determine the rates of extra‐pair paternity (EPP) and conspecific brood parasitism (CBP) in mallards. Maternal and offspring DNA were extracted from feathers in nest material and hatched eggshell membranes. Using 8 microsatelite loci, extra‐pair offspring were detected in 48% of nests and accounted for 9.3% of all offspring. In addition, 10.1% of the offspring were confirmed to result from CBP, and 24% of all nests contained at least 1 offspring from CBP. Rates of conspecific nest parasitism were higher than those of related species, which might have been due to higher breeding densities at our study site. The incidence of EPP was distributed randomly (i.e. did not deviate from bionomial distribution) throughout the population, indicating that variations in pre‐copulatory (e.g. female choice, mate guarding) or post‐copulatory processes (e.g. sperm competition, cryptic female choice) do not affect the distribution of EPP among breeding pairs markedly. Yet, our data provide evidence of variation in the risk of being parasitized among breeding females. The occurrence of CBP and EPP was unaffected by the timing of the breeding attempt or breeding synchrony. 相似文献
77.
Petr Novák Lukáš Žídek Veronika Motáčková Petr Padrta Alžběta Švenková Jean-Marc Nuzillard Libor Krásný Vladimír Sklenář 《Journal of biomolecular NMR》2010,46(2):191-197
S3EPY is a Python extension to the program Sparky written to facilitate the assessment of coupling constants from in-phase/antiphase
and spin-state-selective excitation (S3E) experiments. It enables the routine use of small scalar couplings by automating the coupling evaluation procedure. S3EPY
provides an integrated graphical user interface to programs which outputs graphs and the table of determined couplings. 相似文献
78.
Annett Mikolasch Veronika Hahn Katrin Manda Judith Pump Nicole Illas Dirk Gördes Michael Lalk Manuela Gesell Salazar Elke Hammer Wolf-Dieter Jülich Stephan Rawer Kerstin Thurow Ulrike Lindequist Frieder Schauer 《Amino acids》2010,39(3):671-683
In order to design potential biomaterials, we investigated the laccase-catalyzed cross-linking between l-lysine or lysine-containing peptides and dihydroxylated aromatics. l-Lysine is one of the major components of naturally occurring mussel adhesive proteins (MAPs). Dihydroxylated aromatics are structurally related to 3,4-dihydroxyphenyl-l-alanine, another main component of MAPs. Mass spectrometry and nuclear magnetic resonance analyses show that the ε-amino group of l-lysine is able to cross-link dihydroxylated aromatics. Additional oligomer and polymer cross-linked products were obtained from di- and oligopeptides containing l-lysine. Potential applications in medicine or industry for biomaterials synthesised via the three component system consisting of the oligopeptide [Tyr-Lys]10, dihydroxylated aromatics and laccase are discussed. 相似文献
79.
Martin Flajšhans David Gela Martin Kocour Hana Buchtová Marek Rodina Martin Pšenička Vojtěch Kašpar Veronika Piačková Eliška Sudová Otomar Linhart 《Reviews in Fish Biology and Fisheries》2010,20(3):317-329
Performance and physiological traits and health of spontaneous and induced triploid tench are reviewed. Triploidy is best
induced with cold shock; with triploids exhibiting 13.5–51.5% better weight gain, 2.69–3.94% higher slaughtering value, 20–60%
lower gonadosomatic index, 0.9–4.5% higher dry matter in flesh and up to 107% more flesh fat than diploids, if farmed untill
post sexual maturity. Triploids exhibit more abdominal fat and less polyunsaturated fatty acids of the n-3 and n-6 groups
in the flesh. Triploid females are sterile, while triploid males may produce aneuploid spermatozoa with varying DNA content
(1–1.9n) which may initiate development of embryos. Triploids have milder seasonal dynamics in their erythrocyte profile than
the diploids. Thinner diffusion distance in gills of triploids than in diploids is interpreted as adaptation to lower aerobic
capacity. Triploids show neither stronger tendencies to anatomic malformations, nor have bigger affinity to parasitic diseases
than the diploids. Production of triploid tench could be an economically interesting method of farming to higher marketable
weight, bringing a relatively high product quality. 相似文献