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991.
Giovanna Aronne Carmen Arena Veronica De Micco Manuela Giovanetti Maurizio Buonanno 《Plant biosystems》2018,152(4):863-872
In any biogeographical region, cliffs shelter a larger number of relict species than do the surrounding areas. To develop effective conservation strategies, experimental studies are needed. We focused on Mediterranean coastal cliffs and on Primula palinuri Petagna, an endangered narrow-endemic plant, included in the IUCN Red List and chosen as a key species. The hypothesis was that light intensity and soil water availability could affect survival and growth of juvenile plants. To avoid the bias of other cliff environmental factors and to overcome problems of cliff site inaccessibility, we performed an ex-situ experiment. The effect of two light regimes and two soil water availability conditions on survival and morpho-functional traits were analysed. Plants grown under reduced light intensity and wet soil showed no reduction in survival and performed very well in terms of growth and storage accumulation. By contrast, full light and dry soil proved to be detrimental conditions. Overall results suggested that cliff environmental conditions play an important role in conservation of narrow-endemic species. The decreasing trend of the already severely fragmented populations of P. palinuri and the ongoing increasing aridity is likely to contribute to a further reduction of the already narrow distribution this cliff relict species. 相似文献
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Sofía Racca Elina Welchen Diana E. Gras Danuše Tarkowská Veronika Turečková Veronica G. Maurino Daniel H. Gonzalez 《The Plant journal : for cell and molecular biology》2018,94(1):105-121
We studied the effect of reducing the levels of the mitochondrial electron carrier cytochrome c (CYTc) in Arabidopsis thaliana. Plants with CYTc deficiency have delayed growth and development, and reach flowering several days later than the wild‐type but with the same number of leaves. CYTc‐deficient plants accumulate starch and glucose during the day, and contain lower levels of active gibberellins (GA) and higher levels of DELLA proteins, involved in GA signaling. GA treatment abolishes the developmental delay and reduces glucose accumulation in CYTc‐deficient plants, which also show a lower raise in ATP levels in response to glucose. Treatment of wild‐type plants with inhibitors of mitochondrial energy production limits plant growth and increases the levels of DELLA proteins, thus mimicking the effects of CYTc deficiency. In addition, an increase in the amount of CYTc decreases DELLA protein levels and expedites growth, and this depends on active GA synthesis. We conclude that CYTc levels impinge on the activity of the GA pathway, most likely through changes in mitochondrial energy production. In this way, hormone‐dependent growth would be coupled to the activity of components of the mitochondrial respiratory chain. 相似文献
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Veronica D. Gonzalez Nikolay Samusik Tiffany J. Chen Erica S. Savig Nima Aghaeepour David A. Quigley Ying-Wen Huang Valeria Giangarrà Alexander D. Borowsky Neil E. Hubbard Shih-Yu Chen Guojun Han Alan Ashworth Thomas J. Kipps Jonathan S. Berek Garry P. Nolan Wendy J. Fantl 《Cell reports》2018,22(7):1875-1888
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Veronica J. Masson 《Brittonia》1994,46(3):211-224
The Wabash College Herbarium (WAB), Crawfordsville, Indiana, was donated to The New York Botanical Garden (NY) in 1987. Brief sketches of those who shaped the Wabash College Herbarium, including Albert Reiff Bechtel and Aris Berkeley Donaldson, are presented; photographs of Bechtel and Donaldson are provided. Collectors represented in WAB are listed including the localities and years of the collections and the total number of specimens contributed. 相似文献
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Sonja Stojici? Sanja Milutinovi? Olivera Sarenac Slavoljub Zivkovi? Nina Japundzi?-Zigon 《Biomedizinische Technik》2006,51(4):268-271
This study investigates the contribution of central vasopressin receptors in the modulation of systolic arterial pressure (SAP) and heart rate (HR) response to air-jet stress in conscious Wistar rats equipped with a femoral arterial catheter and intracerebroventricular cannula using novel non-peptide and selective vasopressin V(1a) (SR49059) and V(1b) (SSR149415) antagonists. The effects of stress on SAP and HR were evaluated by measuring the maximal response to stress, the latency of the maximal response, the duration of the recovery period, and the increase in the low frequency (LF) short-term variability component. Stress induced a parallel and almost immediate increase in both SAP and HR, followed by enhanced LF SAP variability in the recovery period. Pretreatment of rats with V(1a) antagonist did not affect the maximal increase or the latency of SAP and HR response to acute stress, but shortened the recovery period of SAP and HR and prevented the increase in LF SAP. The V(1b) antagonist reduced the maximal increase in SAP without affecting HR and their latencies, shortened the recovery period of SAP and inhibited the increase in LF SAP variability. These results indicate that both central V(1a) and V(1b) receptors mediate cardiovascular changes induced by air-jet stress in conscious rats. 相似文献
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Pseudopilin residue E5 is essential for recruitment by the type 2 secretion system assembly platform
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Christian Malosse Nathalie Nadeau Julia Chamot‐Rooke Guy Tran Van Nhieu Olivera Francetic 《Molecular microbiology》2016,101(6):924-941
Type II secretion systems (T2SSs) promote secretion of folded proteins playing important roles in nutrient acquisition, adaptation and virulence of Gram‐negative bacteria. Protein secretion is associated with the assembly of type 4 pilus (T4P)‐like fibres called pseudopili. Initially membrane embedded, pseudopilin and T4 pilin subunits share conserved transmembrane segments containing an invariant Glu residue at the fifth position, E5. Mutations of E5 in major T4 pilins and in PulG, the major pseudopilin of the Klebsiella T2SS abolish fibre assembly and function. Among the four minor pseudopilins, only PulH required E5 for secretion of pullulanase, the substrate of the Pul T2SS. Mass‐spectrometry analysis of pili resulting from the co‐assembly of PulGE5A variant and PulGWT ruled out an E5 role in pilin processing and N‐methylation. A bacterial two‐hybrid analysis revealed interactions of the full‐length pseudopilins PulG and PulH with the PulJ‐PulI‐PulK priming complex and with the assembly factors PulM and PulF. Remarkably, PulGE5A and PulHE5A variants were defective in interaction with PulM but not with PulF, and co‐purification experiments confirmed the E5‐dependent interaction between native PulM and PulG. These results reveal the role of E5 in a recruitment step critical for assembly of the functional T2SS, likely relevant to T4P assembly systems. 相似文献