New Two-Component Pyrene Probes Based on Oligo(2'-O-Methylribonucleotides) for microRNA Detection

Russian Journal of Bioorganic Chemistry - New two-component pyrene probes based on oligo(2'-O-methylribonucleotides) for microRNA detection have been designed. They contain the PyA-modified...     

Oligonucleotide-Peptide Conjjugates for RNA Cleavage

Abstract

Directed cleavage of RNA phosphodiester bonds by peptidyloligodeoxyribonucleotide containing the peptide residues with the alternated hydrophobic and basic amino acids was demonstrated.     

Two-component 10-23 DNA enzymes

A new strategy for engineering of catalytic two-component constructions based on 10-23 DNAzyme was proposed. The using of a combination of shortened DNAzyme with 2'-O-methyl oligomers as effectors significantly increased the catalytic activity of this DNAzyme.     

Stabilization of DNA double and triple helices by conjugation of minor groove binders to oligonucleotides

New conjugates containing two parallel or antiparallel carboxamide minor groove binders (MGB) attached to the same terminal phosphate of one oligonucleotide strand were synthesized. The conjugates interact with their target DNA stronger than the individual components. Effect of conjugated MGB on DNA duplex and triplex stability and their sequence specificity was demonstrated on the short oligonucleotide duplexes and on the triplex formed by model 16-mer oligonucleotide with HIV polypurine tract.     

Oligoribonucleotides with functionalized nucleobases as new modifiers of biopolymers

Synthesis and evaluation of hybridization and modification abilities of the new types of photoactivatable oligoribonucleotide conjugates are presented.     

RNase P-Guiding Peptide Conjugates of Oligo(2'-O-methylribonucleotides) as Prospective Antibacterial Agents

Russian Journal of Bioorganic Chemistry - A novel variant of the synthesis of 3'- and 5'-peptide conjugates of oligo(2'-O-methylribonucleotides) has been developed using thiol-maleimide...     

Cleavage of RNA in hybrid duplexes by theE. coli ribonuclease H: II. Substrate properties of oligonucleotides containing nonnucleotide linkers

The 20-mer bridged oligodeoxynucleotides containing short oligomers joined by the hexamethylenediol and hexaethylene glycol linkers were shown to form complementary DNA/DNA and RNA/DNA complexes whose thermostability depends on the length and number of the nonnucleotide linkers. Hybrid complexes of the bridged oligonucleotides proved to be substrates for theE. coli ribonuclease H. The presence of one-three nonnucleotide linkers in a 20-mer decreased the hydrolysis efficacy only 1.2–1.4-fold. It is the composition of the RNA cleavage products that was influenced the most significantly by the nonnucleotide linkers. RNase H simultaneously hydrolyzed the RNA 3′-ends of each hybrid duplex involving a bridged oligonucleotide. The presence of an inverted 3′-3′-phosphodiester bond at the 3′-end of the oligodeoxyribonucleotide only slightly affected the RNase H activity. For the previous report, see [1].     

Comparative Study of Modification of DNA and RNA by Oligo(2′-O-Methylribonucleotide) Derivatives

Abstract

The results of modification of the model DNA and RNA targets by the alkylating derivatives of 2′-O-methylribo-, ribo-, and deoxyhexanucleotides in the presence and absence of effectors (N-(2-hydroxyethyl)phenazinium derivatives of the same type of octanucleotides) are presented. It has been shown that the alkylating 4(N-methyl-N-2-chloroethyl-)benzylmethylamidophosphate derivatives of oligo(2′-O-methylribonucleotides) are the high effective reagents for the site specific modification of nucleic acids especially RNA.

     

Conjugates of RNase P-Guiding Oligonucleotides with Oligo(N-Methylpyrrole) as Prospective Antibacterial Agents

Russian Journal of Bioorganic Chemistry - The conjugates of RNase P guiding oligo(2'-O-methylribo)- and oligodeoxyribonucleotides (EGS oligonucleotides) with oligo(N-methylpyrrole) have been...