排序方式: 共有41条查询结果,搜索用时 421 毫秒
31.
Shaik Nayab Rasool Shaik Shabeena Culala Rajasekharaudayar Kesavulu Singarapu Babu Asanapuram Mohan Babu Vemula Venkatramu 《Luminescence》2022,37(5):784-790
Novel erbium(III) ion-doped borate-based glasses (Er3+:BCNF) by conventional melt-quenching technique were designed and synthesized. The glasses were characterized for their structural, vibrational and spectroscopic properties. The nephelauxetic ratio, bonding parameters, and Judd–Ofelt (JO) intensity parameters (Ωλ λ = 2, 4 and 6) were determined by using absorption spectrum of 1 mol% Er2O3 doped glass. These JO parameters were utilized to derive radiative properties for various excited states of erbium(III) ions. Emission cross-section for 4I13/2 → 4I15/2 transition of erbium(III) ions was computed through McCumber theory. The decay curves for (2H11/2, 4S3/2) and 4I13/2 levels were recorded and analysed. All the results of Er3+:BCNF glasses revealed that the studied glasses are efficient and thermally stable and could be suitable for display devices, optical amplification and green laser applications. 相似文献
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Abishad Padikkamannil Vergis Jess Unni Varsha Ram Vemula Prasastha Niveditha Pollumahanti Yasur Jyothsana Juliet Sanis John Lijo Byrappa Kullaiya Nambiar Prejit Kurkure Nitin Vasantrao Barbuddhe Sukhadeo Baliram Rawool Deepak Bhiwa 《Probiotics and antimicrobial proteins》2022,14(5):904-914
Probiotics and Antimicrobial Proteins - The present study was envisaged to employ the green synthesis and characterization of silver nanoparticles (AgNPs) using the potential probiotic strain... 相似文献
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Julia C. Cronin John Wunderlich Stacie K. Loftus Todd D. Prickett Xiaomu Wei Katie Ridd Swapna Vemula Allison S. Burrell Neena S. Agrawal Jimmy C. Lin Carolyn E. Banister Phillip Buckhaults Steven A. Rosenberg Boris C. Bastian William J. Pavan Yardena Samuels 《Pigment cell & melanoma research》2009,22(4):435-444
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Aprajita Kumari Pooja Singh Vemula Chandra Kaladhar Manbir Debarati Paul Pradeep Kumar Pathak Kapuganti Jagadis Gupta 《Plant, cell & environment》2022,45(1):178-190
An important and interesting feature of rice is that it can germinate under anoxic conditions. Though several biochemical adaptive mechanisms play an important role in the anaerobic germination of rice but the role of phytoglobin-nitric oxide cycle and alternative oxidase pathway is not known, therefore in this study we investigated the role of these pathways in anaerobic germination. Under anoxic conditions, deepwater rice germinated much higher and rapidly than aerobic condition and the anaerobic germination and growth were much higher in the presence of nitrite. The addition of nitrite stimulated NR activity and NO production. Important components of phytoglobin-NO cycle such as methaemoglobin reductase activity, expression of Phytoglobin1, NIA1 were elevated under anaerobic conditions in the presence of nitrite. The operation of phytoglobin-NO cycle also enhanced anaerobic ATP generation, LDH, ADH activities and in parallel ethylene levels were also enhanced. Interestingly nitrite suppressed the ROS production and lipid peroxidation. The reduction of ROS was accompanied by enhanced expression of mitochondrial alternative oxidase protein and its capacity. Application of AOX inhibitor SHAM inhibited the anoxic growth mediated by nitrite. In addition, nitrite improved the submergence tolerance of seedlings. Our study revealed that nitrite driven phytoglobin-NO cycle and AOX are crucial players in anaerobic germination and growth of deepwater rice. 相似文献
36.
Sarkar D Vemula PK Teo GS Spelke D Karnik R Wee le Y Karp JM 《Bioconjugate chemistry》2008,19(11):2105-2109
Covalently conjugated sialyl Lewis X (SLeX) on the mesenchymal stem cell (MSC) surface through a biotin-streptavidin bridge imparts leukocyte-like rolling characteristics without altering the cell phenotype and the multilineage differentiation potential. We demonstrate that the conjugation of SLeX on the MSC surface is stable, versatile, and induces a robust rolling response on P-selectin coated substrates. These results indicate the potential to increase the targeting efficiency of any cell type to specific tissue. 相似文献
37.
Seshagirirao K Leelavathi C Sasidhar V 《Journal of biochemistry and molecular biology》2005,38(3):370-372
A cross-linked leucaena (Leucaena leucocephala) seed gum (CLLSG) matrix was prepared for the isolation of galactose-specific lectins by affinity chromatography. The matrix was evaluated for affinity with a known galactose-specific lectin from the seeds of snake gourd (Trichosanthes anguina). The matrix preparation was simple and inexpensive when compared to commercial galactose-specific matrices (i.e. about 1.5 US dollars/100 ml of matrix). The current method is also useful for the demonstration of the affinity chromatography technique in laboratories. Since leucaena seeds are abundant and inexpensive, and the matrix preparation is easy, CLLSG appears to be a promising tool for the separation of galactose-specific lectins. 相似文献
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Activation of intrinsic and extrinsic proapoptotic signaling pathways in interleukin-18-mediated human cardiac endothelial cell death 总被引:4,自引:0,他引:4
Chandrasekar B Vemula K Surabhi RM Li-Weber M Owen-Schaub LB Jensen LE Mummidi S 《The Journal of biological chemistry》2004,279(19):20221-20233
Endothelial cells are the primary targets of circulating immune and inflammatory mediators. We hypothesize that interleukin-18, a proinflammatory cytokine, induces endothelial cell apoptosis. Human cardiac microvascular endothelial cells (HCMEC) were treated with interleukin (IL) 18. mRNA expression was analyzed by ribonuclease protection assay, protein levels by immunoblotting, and cell death by enzyme-linked immunosorbent assay and fluorescence-activated cell sorter analysis. We also investigated the signal transduction pathways involved in IL-18-mediated cell death. Treatment of HCMEC with IL-18 increases 1) NF-kappaB DNA binding activity; 2) induces kappaB-driven luciferase activity; 3) induces IL-1beta and TNF-alpha expression via NF-kappaB activation; 4) inhibits antiapoptotic Bcl-2 and Bcl-X(L); 5) up-regulates proapoptotic Fas, Fas-L, and Bcl-X(S) expression; 6) induces fas and Fas-L promoter activities via NF-kappaB activation; 7) activates caspases-8, -3, -9, and BID; 8) induces cytochrome c release into the cytoplasm; 9) inhibits FLIP; and 10) induces HCME cell death by apoptosis as seen by increased annexin V staining and increased levels of mono- and oligonucleosomal fragmented DNA. Whereas overexpression of Bcl-2 significantly attenuated IL-18-induced endothelial cell apoptosis, Bcl-2/Bcl-X(L) chimeric phosphorothioated 2'-MOE-modified antisense oligonucleotides potentiated the proapoptotic effects of IL-18. Furthermore, caspase-8, IKK-alpha, and NF-kappaB p65 knockdown or dominant negative IkappaB-alpha and dominant negative IkappaB-beta or kinase dead IKK-beta significantly attenuated IL-18-induced HCME cell death. Effects of IL-18 on cell death are direct and are not mediated by intermediaries such as IL-1beta, tumor necrosis factor-alpha, or interferon-gamma. Taken together, our results indicate that IL-18 activates both intrinsic and extrinsic proapoptotic signaling pathways, induces endothelial cell death, and thereby may play a role in myocardial inflammation and injury. 相似文献
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Chellappa R Kandasamy P Oh CS Jiang Y Vemula M Martin CE 《The Journal of biological chemistry》2001,276(47):43548-43556
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Direct role for the Rpd3 complex in transcriptional induction of the anaerobic DAN/TIR genes in yeast
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Saccharomyces cerevisiae adapts to hypoxia by expressing a large group of "anaerobic" genes. Among these, the eight DAN/TIR genes are regulated by the repressors Rox1 and Mot3 and the activator Upc2/Mox4. In attempting to identify factors recruited by the DNA binding repressor Mot3 to enhance repression of the DAN/TIR genes, we found that the histone deacetylase and global repressor complex, Rpd3-Sin3-Sap30, was not required for repression. Strikingly, the complex was instead required for activation. In addition, the histone H3 and H4 amino termini, which are targets of Rpd3, were also required for DAN1 expression. Epistasis tests demonstrated that the Rpd3 complex is not required in the absence of the repressor Mot3. Furthermore, the Rpd3 complex was required for normal function and stable binding of the activator Upc2 at the DAN1 promoter. Moreover, the Swi/Snf chromatin remodeling complex was strongly required for activation of DAN1, and chromatin immunoprecipitation analysis showed an Rpd3-dependent reduction in DAN1 promoter-associated nucleosomes upon induction. Taken together, these data provide evidence that during anaerobiosis, the Rpd3 complex acts at the DAN1 promoter to antagonize the chromatin-mediated repression caused by Mot3 and Rox1 and that chromatin remodeling by Swi/Snf is necessary for normal expression. 相似文献