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181.
182.
Immunohistochemical localization of acetylcholine receptors at human endplates using a monoclonal antibody 总被引:1,自引:0,他引:1
L M Smit H Veldman F G Jennekens 《The journal of histochemistry and cytochemistry》1987,35(5):613-617
We describe a simple indirect immunohistochemical method for localization of acetylcholine receptors (AChR) in motor endplates at the light and electron microscopic level. This method involves the use of a monoclonal antibody directed against the main immunogenic region (MIR) of AChRs and is applicable to periodate-lysine-paraformaldehyde (PLP)-fixed tissue. We discuss the advantages of this method, as compared with the alpha-bungarotoxin-immunoperoxidase technique, and stress its value for diagnostic investigations of motor point biopsies from patients with neuromuscular transmission disorders. 相似文献
183.
Repeated epilation (Er) is a radiation-induced, autosomal, incomplete dominant mutation in mice which is expressed in heterozygotes but is lethal in the homozygous condition. Many effects of the mutation occur in skin: the epidermis in Er/Er mice is adhesive (oral and nasal orifices fuse, limbs adhere to the body wall), hyperplastic, and fails to undergo terminal differentiation. Skin from fetal +/+, Er/+ and Er/Er mice at ages pre- and postkeratinization examined by light, scanning, and transmission electron microscopy showed marked abnormalities in tissue architecture, differentiation, and cell structure; light and dark basal epidermal cells were separated by wide intercellular spaces, joined by few desmosomes, and contained phagolysomes. The numbers of spinous, granular, and superficial layers were highly variable within any given region and among various regions of the body. In some areas, 2-8 layers of granular cells, containing large or diminutive keratohyalin granules, extended to the epidermal surface; in others, the granular layers were covered by several layers of partially keratinized or nonkeratinized cells. In rare instances, a single or small group of cornified cells was present among the granular layers but was not associated with the epidermal surface. Both the granular and nonkeratinized/partially keratinized upper epidermal layers Er/Er skin gave positive immunofluorescence with antiserum to the histidine-rich, basic protein, filaggrin. Proteins in epidermal extracts from +/+, Er/+ and Er/Er mice were separated and identified by radio- and immunolabeling techniques. The Er/Er extract was missing a 26.5- kdalton protein and had an altered ratio of bands in the keratin region. The 26.5-kdalton band was histidine-rich and cross-reacted with the antiserum to rat filaggrin. Several high molecular weight bands present in both Er/Er and +/+ extracts also reacted with the antiserum. These are presumed to be the precursors of filaggrin and to account for the immunofluorescence om Er/Er epidermis even though the product protein is absent. The morphologic and biochemical data indicated that the genetic defect has a general and profound influence on epidermal differentiation, including alteration of two proteins (filaggrin and keratin) important in normal terminal differentiation, tissue architecture, and cytology. Identification of epidermal abnormalities at early stages of development (prekeratinization) and defective structure of other tissues and gross anatomy suggest that the mutation is responsible for a defect in same regulatory step important in many processes of differentiation and development. 相似文献
184.
Brigitte M. Jockusch Henk Veldman Gareth W. Griffiths Bernard A. Van Oost Frans G. I. Jennekens 《Experimental cell research》1980,127(2):409-420
A biopsy of skeletal muscle taken from a child with the clinical symptoms of congenital nemaline myopathy was studied. Light and electron microscopy revealed rod-like structures within the muscle fibres, and thus confirmed the clinical diagnosis. Indirect immunofluorescence, using specific antibodies against actin and desmin (both derived from chicken gizzard) as well as against α-actinin and tropomyosin (both from porcine skeletal muscle) revealed that the rods consist of massive accumulations of α-actinin. Desmin seems to be peripherally associated with the rods. Anti-actin and anti-tropomyosin did not stain the rods; however, a masking effect could not be ruled out. These findings support previous hypotheses that nemaline rods can be taken to be lateral-polymers of normal Z-disks. 相似文献
185.
Some characteristics of processing sites in ribosomal precursor RNA of yeast. 总被引:26,自引:15,他引:11 下载免费PDF全文
The DNA sequences of the intergenic region between the 17S and 5.8S rRNA genes of the ribosomal RNA operon in yeast has been determined. In this region the 37S ribosomal precursor RNA is specifically cleaved at a number of sites in the course of the maturation process. The exact position of these processing sites has been established by sequence analysis of the terminal fragments of the respective RNA species. There appears to be no significant complementarity between the sequences surrounding the two termini of the 18S secondary precursor RNA nor between those surrounding the two termini of 17S mature rRNA. This finding implies that the processing of yeast 37S ribosomal precursor RNA is not directed by a double-strand specific ribonuclease previously shown to be involved in the processing of E. coli ribosomal precursor RNA [see Refs 1,2]. The processing sites of yeast ribosomal precursor RNA described in the present paper are all flanked at one side by a very [A+T]-rich sequence. In addition, sequence repeats are found around the processing sites in this precursor RNA. Finally, sequence homologies are present at the 3'-termini [6 nucleotides] and the 5'-termini [13 nucleotides] of a number of mature rRNA products and intermediate ribosomal RNA precursors. These structural features are discussed in terms of possible recognition sites for the processing enzymes. 相似文献
186.
J E Veldman P Nieuwenhuis F J Keuning 《Virchows Archiv. B, Cell pathology including molecular pathology》1980,33(2):127-138
B-cell reactivity, as expressed in a plasma cell reaction may be part of a graft-versus-host or host-versus-graft reaction. Cell transfer experiments were able to prove this. Histological evidence is presented for the occurrence of the so-called "allogeneic effect" during a graft-versus-host or host-versus-graft reaction in the rabbit spleen. Cell suspensions containing both T- and B-cells give rise to a cellular and a humoral immunity reaction component upon transfer to histoincompatible recipients. This can also occur during a graft-versus-host reaction. This B-cell response is T-cell dependent. 相似文献
187.
We isolated from the endogenous polyprenyl-phospho-sugar pool of
Mycobacterium smegmatis two mannose-containing compounds, i.e., a partially
saturated C35-octahydroheptaprenyl-P-mannose and a fully unsaturated
C50-decaprenyl-P-mannose. The relative amount of C35- polyprenyl-P-mannose
in mycobacterial cells was comparable to that of decaprenyl- P-pentoses
and, at least, an order of magnitude higher than that of
C50-decaprenyl-P-mannose. The major form of mycobacterial
polyprenyl-P-mannose was structurally characterized by combined gas
chromatography-mass spectrometry, fast-atom bombardment tandem mass
spectrometry and proton-nuclear magnetic resonance spectroscopy as beta-
d-mannopyranosyl-monophospho-(C35)octahydroheptapren ol of which all three
isoprene units have Z ( cis ) configuration. The differences in the
structure and cellular concentrations of the mycobacterial mannosyl-
P-polyprenols reflect distinct biochemical pathways of the two compounds
and suggest the existence of specific GDP-Man:polyprenyl-P
mannosyltransferases (synthetases) able to distinguish between C35-
octahydroheptaprenyl- and C50-decaprenyl- phosphates of mycobacteria. Since
the 6'-O-mycoloylated form of C35-octahydroheptaprenyl-P-mannose isolated
from M. smegmatis is apparently involved in mycolate rather than mannosyl
transfer reactions, we speculate that a catabolic pathway responsible for
degradation of C35-P-mannose and recycling C35- octahydroheptaprenyl
phosphate might exist in mycobacteria.
相似文献
188.
Veldman RJ Sietsma H Klappe K Hoekstra D Kok JW 《Biochemical and biophysical research communications》1999,266(2):492-496
In the present study we show that neutral hexanoyl-(glyco)sphingolipids inhibit P-glycoprotein (Pgp) activity in human ovarian 2780AD cells. By contrast, hexanoylceramide and the gangliosides GM(3) and GM(2) had no effect on Pgp activity, whereas sphingosine had a stimulating effect. In the case of hexanoylglucosylceramide, inhibition of Pgp activity by was reflected by a regained doxorubicin sensitivity of cells, which were grown in medium supplemented with the lipid. Our results lead to the conclusion that a direct transmodulation of Pgp activity by glycolipids occurs, depending on lipid headgroup structure, which can result in reduced resistance to the chemotherapeutic agent doxorubicin. 相似文献
189.
Antimicrobial Resistance Profiles of Campylobacter jejuni Isolates from Wild Birds in Sweden 下载免费PDF全文
Jonas Waldenstr?m Dik Mevius Kees Veldman Tina Broman Dennis Hasselquist Bj?rn Olsen 《Applied microbiology》2005,71(5):2438-2441
In order to determine the occurrence and frequency of resistant strains of the bacterium Campylobacter jejuni and to establish baseline MICs in isolates from an environmental reservoir, the resistance profiles of 10 antimicrobial substances were determined for 137 C. jejuni isolates from wild birds in Sweden. Observed MICs were generally low, with only low to moderate incidence of resistance to the tested compounds. One isolate, however, was resistant to nalidixic acid and ciprofloxacin, indicating that quinolone-resistant genotypes of C. jejuni have the potential to spread to wild bird hosts. 相似文献
190.
目的探讨吸氧预处理对大鼠脑缺血再灌注损伤的保护作用。方法通过大鼠局灶脑缺血再灌注损伤模型,采用SOD、MDA测定、电镜及神经行为学检查的方法,观察吸氧预处理对大鼠脑缺血再灌注损伤后SOD、MDA、神经行为学评分及脑组织病理变化。结果吸氧预处理组SOD活力高于对照组(P<0.05),MDA含量、神经行为学评分均低于对照组(P<0.05),脑组织超微结构损伤均减轻。结论吸氧预处理对大鼠脑缺血再灌注损伤有保护作用。 相似文献