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Oseni Ojo Michael Nailwal Tapan K. Pande Veena 《In vitro cellular & developmental biology. Plant》2022,58(3):479-488
In Vitro Cellular & Developmental Biology - Plant - This study aimed to investigate indirect micropropagation of Mansonia altissima using nodal explant and confirmation of genetic stability of... 相似文献
464.
Anil Kumar Varun Kumar Arvind Kumar Dubey Mohd Akram Ansari Shiv Narayan Meenakshi Sanoj Kumar Vivek Pandey Veena Pande Indraneel Sanyal 《Physiology and Molecular Biology of Plants》2021,27(5):923
Glutaredoxins (Grxs) are short, cysteine-rich glutathione (GSH)-mediated oxidoreductases. In this study, a chickpea (Cicer arietinum L.) glutaredoxin [LOC101493651 (CaGrx)] gene has been selected based on screening experiments with two contrasting varieties of chickpea, PUSA-362 (drought-tolerant) and ICC-1882 (drought-sensitive) under drought and salinity. The tolerant variety showed higher CaGrx gene expression, as compared to less in the sensitive variety, under both the stresses. The CaGrx gene was then over-expressed in Arabidopsis thaliana and were exposed to drought and salinity. The over-expression of CaGrx elevated the activity of glutaredoxin, which induced antioxidant enzymes (glutathione reductase; GR, glutathione peroxidase; GPX, catalase; CAT, ascorbate peroxidase; APX, glutathione-S-transferase; GST, superoxide dismutase; SOD, monodehydroascorbate reductase; MDHAR, and dehydroascorbate reductase; DHAR), antioxidants (GSH and ascorbate) and stress-responsive amino acids (cysteine and proline). Enhancement in the antioxidant defense system possibly administered tolerance in transgenics against both stresses. CaGrx reduced stress markers (H2O2, TBARS, and electrolyte leakage) and enhanced root growth, seed germination, and survival against both stresses. The physiological parameters (net photosynthesis; PN, water use efficiency; WUE, stomatal conductance; gs, transpiration; E, electron transport rate; ETR, and photochemical quenching; qP), chlorophylls and carotenoids, were improved in the transgenics during both stresses, that maintained the photosynthetic apparatus and protected the plants from damage. The enhanced activity of the cysteine biosynthesis enzyme, o-acetylserine (thiol) lyase (OAS-TL), increased the cysteine level in the transgenics, which elevated glutathione biosynthesis to maintain the ascorbate–glutathione cycle under both stresses. This investigation verified that the CaGrx gene provides tolerance against salinity and drought, maintaining physiological and morphological performances, and could be exploited for genetic engineering approaches to overcome both the stresses in various crops.Supplementary InformationThe online version contains supplementary material available at 10.1007/s12298-021-00999-z. 相似文献
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P. Therese Lang Scott R. Brozell Sudipto Mukherjee Eric F. Pettersen Elaine C. Meng Veena Thomas Robert C. Rizzo David A. Case Thomas L. James Irwin D. Kuntz 《RNA (New York, N.Y.)》2009,15(6):1219-1230
With an increasing interest in RNA therapeutics and for targeting RNA to treat disease, there is a need for the tools used in protein-based drug design, particularly DOCKing algorithms, to be extended or adapted for nucleic acids. Here, we have compiled a test set of RNA–ligand complexes to validate the ability of the DOCK suite of programs to successfully recreate experimentally determined binding poses. With the optimized parameters and a minimal scoring function, 70% of the test set with less than seven rotatable ligand bonds and 26% of the test set with less than 13 rotatable bonds can be successfully recreated within 2 Å heavy-atom RMSD. When DOCKed conformations are rescored with the implicit solvent models AMBER generalized Born with solvent-accessible surface area (GB/SA) and Poisson–Boltzmann with solvent-accessible surface area (PB/SA) in combination with explicit water molecules and sodium counterions, the success rate increases to 80% with PB/SA for less than seven rotatable bonds and 58% with AMBER GB/SA and 47% with PB/SA for less than 13 rotatable bonds. These results indicate that DOCK can indeed be useful for structure-based drug design aimed at RNA. Our studies also suggest that RNA-directed ligands often differ from typical protein–ligand complexes in their electrostatic properties, but these differences can be accommodated through the choice of potential function. In addition, in the course of the study, we explore a variety of newly added DOCK functions, demonstrating the ease with which new functions can be added to address new scientific questions. 相似文献
466.
Akshay Narkar Blake A. Johnson Pandurang Bharne Jin Zhu Veena Padmanaban Debojyoti Biswas Andrew Fraser Pablo A. Iglesias Andrew J. Ewald Rong Li 《Cell reports》2021,34(12):108892
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The Protein Journal - Tuberculosis (TB) is an airborne infectious disease caused by Mycobacterium tuberculosis (M.tb) whose natural history traces back to 70,000 years. TB remains a major... 相似文献
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Veena Jain Nisha Garg H. S. Nainawatee 《World journal of microbiology & biotechnology》1990,6(4):434-436
Rhizobium meliloti-alfalfa (Medicago sativa) symbiosis was influenced by the rhizospheric application of naringenin which increased nodule number, nodule weight (2-to 8-fold) and nitrogenase activity. Plant blomass and total nitrogen content also increased by 60 to 72%. The enhancing effects of naringenin were more pronounced if it was applied at the early vegetative stage of plant growth or at the time of sowing. 相似文献