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排序方式: 共有108条查询结果,搜索用时 109 毫秒
51.
52.
T. P. Vavilova I. V. Tarasenko A. E. Medvedev I. G. Ostrovskaya 《Biochemistry (Moscow) Supplemental Series B: Biomedical Chemistry》2010,4(4):383-385
The content of basic fibroblast growth factor (bFGF) and also interleukins 1β and 6 (IL-1β and IL-6) has been investigated
in rat buccal mucosa after its surgical injury by an erbium laser (Er:YAG laser) and a scalpel. The laser emission caused
a sharp increase in the content of these regulators on the second day after treatment followed by decrease observed on the
seventh day. These results may reflect synergistic effect of these peptide regulators in the wound defect. Changes in time-course
of bFGF, IL-1β and IL-6 release in the wound formed by the laser beam compared with the wound induced by the cutting instrument
may promote earlier appearance of the proliferation phase. 相似文献
53.
Yeast strains belonging to the genera Candida and Hansenula were shown to differ in their susceptibility to the action of protein antibiotics produced by the yeasts Williopsis and Zygowilliopsis. This finding can be used as an additional criterion for yeast identification. 相似文献
54.
Usenko TS Emel'ianov AK Iakimovskiĭ AF Bogan'kova NA Vavilova TV Shvartsman AL Pchelina SN 《Tsitologiia》2012,54(1):44-48
Mutations in the Leucine Reach Repeat Kinase 2 (LRRK2) gene are the most frequent cause of familial Parkinson's disease (PD). Although the precise physiological and pathological role of LRRK2 is unclear, a direct link between mutant LRRK2 and apoptosis has been suggested. Using flow cytometric analysis (PI+Annexin V(FITC)) we showed increased spontaneous apoptosis of peripheral blood lymphocytes in patients with LRRK.2-associated PD compared to controls after 24 (P < 0.016) and 48 (P < 0.031 ) h of incubation (5 % CO2, 37 degrees C). We found the increased FAS mRNA level in peripheral blood lymphocytes of patients with LRRK2-associated PD compared to controls (P < 0.05) and to sporadic PD (sPD) (P < 0.002). Significant difference in FAS expression between patients with LRRK2-associated PD and controls remained after three years and was detected after 1 and 24 h during lymphocyte incubation (P < 0.03 and 0.05, respectively). Increased spontaneous lymphocytes apoptosis along to increased FAS expression in patients with LRRK2-associated PD suggest that LRRK2 mutations may lead to the activation of extrinsic apoptotic way. 相似文献
55.
I A Lapaeva B A Rebentish S M Mebel' V N Kalinin E Iu Vavilova 《Zhurnal mikrobiologii, epidemiologii, i immunobiologii》1985,(12):35-38
Possible causes limiting the multiplication of Bordetella phages or inducing their restriction, such as the influence of lysogenic immunity and the restriction-modification (R-M) system or the incompatibility of the receptor apparatus, have been studied. The limitation of the multiplication of phages by some B. bronchiseptica and B. pertussis strains has been shown to be due to the presence of the R-M system and lysogenic immunity. In five B. bronchiseptica strains and two B. pertussis strains site-specific endonucleases (restrictases) with Hind III specificity have been detected. One B. bronchiseptica strain without the R-M system has been detected. B. bronchiseptica strains producing site-specific endonucleases are practically nonpathogenic for humans, grow in common culture media and selectively produce only one restrictase, type Hind III, which guarantees from the admixture of other specific endonucleases. The B. parapertussis strains under study (altogether 100 strains) have not been found to limit the multiplication of Bordetella test phages. The absence of site-specific endonucleases has also been confirmed biochemically. These strains are recommended as indicator strains for the multiplication of Bordetella phages. 相似文献
56.
The biological activity of the endocrine secretum fraction isolated from the rat duodenum is determined. The fraction with the molecular weight about 3 kDa is found to possess the factor which inhibits the Na+,K+-ATPase activity of enterocytes. It is found that the inhibitory factor secretum depends on the solution which irrigates the duodenum cavity. The possible regulatory role of the intestine inhibitory factor is under discussion. 相似文献
57.
E. B. Kuchmenko D. N. Petukhov G. V. Donchenko L. S. Mkhitaryan S. V. Tymoshchuk N. A. Strutynskaya G. L. Vavilova V. F. Sagach 《Biochemistry (Moscow) Supplemental Series B: Biomedical Chemistry》2009,3(4):386-392
Aging is accompanied by changes in activity of electron-transport enzyme complexes in myocardial mitochondria of old rats
and by increased sensitivity of the mitochondrial permeability transition pore (MPTP) to inductors of its opening (Ca2+ and phenylarsine oxide). We also observed activation of lipid and protein free-radical peroxidation processes. Administration
of a complex of biologically active substances that included precursors and modulators of coenzyme Q biosynthesis (α-tocopherol
acetate, 4-hydroxybenzoic acid, and methionine) caused the increase in coenzyme Q content, correction of functional activity
of mitochondrial electron-transport chain enzyme complexes, the decrease in intensity of lipid and protein free-radical peroxidation
in the heart mitochondria and the decrease in sensitivity of mitochondrial permeability transition pore to inductors of its
opening. This complex may be recommended for treatment of mitochondrial dysfunction in various pathologies of cardiovascular
system, including in aging. 相似文献
58.
M Ozkan SG Desai Y Zhang DM Stevenson J Beane EA White ML Guerinot LR Lynd 《Journal of industrial microbiology & biotechnology》2001,27(5):275-280
Characteristics of 13 newly isolated thermophilic, anaerobic, and cellulolytic strains were compared with previously described
strains of Clostridium thermocellum: ATCC 27405 and JW20 (ATCC 31549). Colony morphology, antibiotic sensitivity, fermentation end-products, and cellulose degradation
were documented. All 13 strains were sensitive to erythromycin (5 μg/ml) and chloramphenicol (25 μg/ml), and all strains but
one were sensitive to kanamycin (20 μg/ml). Polymerase chain reaction (PCR) amplification using primers based on gene sequences
from C. thermocellum ATCC 27405 was successful for all 13 strains in the case of the hydrogenase gene and 11 strains in the case of phosphotransacetylase/acetate
kinase genes. Ten strains amplified a product of the expected size with primers developed to be specific for C. thermocellum 16SrRNA primers. Two of the 13 strains did not amplify any product with the PCR primers designed for the phosphotransacetylase/acetate
kinase and 16SrRNA primers. A MboI-like GATC- recognizing restriction activity was present in all of the five strains examined. The results of this study have
several positive implications with respect to future development of a transformation system for cellulolytic thermophiles.
Journal of Industrial Microbiology & Biotechnology (2001) 27, 275–280.
Received 12 September 2000/ Accepted in revised form 20 November 2000 相似文献
59.
60.
Serebryanyi V. A. Vavilova E. A. Chulkin A. M. Vinetskii Yu. P. 《Applied Biochemistry and Microbiology》2002,38(5):420-426
The complete gene xylA that encodes endo-1,4--xylanase secreted byPenicillium canescens was cloned and sequenced. The coding region of the gene is separated by eight introns. The protein comprises 302 amino acids of the mature protein and 25 amino acids of the signal peptide. The xylanase of P. canescens belongs to the glycosyl hydrolase family 10. Nucleotide sequences for binding catabolite repression protein CREA and transactivator protein were detected in the promoter region. A set of multicopy strains displaying a seven to eightfold increase in xylanase yield was obtained. The fraction of xylanase in most productive strains amounted to 30–50% of the total secreted protein. 相似文献