全文获取类型
收费全文 | 2378篇 |
免费 | 161篇 |
专业分类
2539篇 |
出版年
2023年 | 13篇 |
2022年 | 31篇 |
2021年 | 47篇 |
2020年 | 31篇 |
2019年 | 40篇 |
2018年 | 61篇 |
2017年 | 52篇 |
2016年 | 67篇 |
2015年 | 94篇 |
2014年 | 123篇 |
2013年 | 173篇 |
2012年 | 167篇 |
2011年 | 150篇 |
2010年 | 108篇 |
2009年 | 88篇 |
2008年 | 135篇 |
2007年 | 157篇 |
2006年 | 112篇 |
2005年 | 130篇 |
2004年 | 107篇 |
2003年 | 95篇 |
2002年 | 96篇 |
2001年 | 37篇 |
2000年 | 39篇 |
1999年 | 31篇 |
1998年 | 23篇 |
1997年 | 15篇 |
1996年 | 21篇 |
1995年 | 22篇 |
1994年 | 5篇 |
1993年 | 12篇 |
1992年 | 28篇 |
1991年 | 24篇 |
1990年 | 30篇 |
1989年 | 26篇 |
1988年 | 20篇 |
1987年 | 14篇 |
1986年 | 8篇 |
1985年 | 12篇 |
1984年 | 7篇 |
1983年 | 7篇 |
1982年 | 9篇 |
1981年 | 9篇 |
1980年 | 14篇 |
1979年 | 8篇 |
1978年 | 17篇 |
1977年 | 3篇 |
1976年 | 3篇 |
1970年 | 2篇 |
1969年 | 2篇 |
排序方式: 共有2539条查询结果,搜索用时 0 毫秒
61.
Computational methods have played a key role in elucidating the various three-dimensional structures of oligosaccharides. Such structural information, together with other experimental data, leads to a better understanding of the role of oligosaccharide in various biological processes. The disialoside Neu5Ac-alpha2-->8-Neu5Ac appears as the terminal glycan in glycoproteins and glycolipids, and is known to play an important role in various events of cellular communication. Neurotoxins such as botulinum and tetanus require Neu5Ac-alpha2 --> 8-Neu5Ac for infecting the host. Glycoconjugates containing this disialoside and the enzymes catalyzing their biosynthesis are also regulated during cell growth, development, and differentiation. Unlike other biologically relevant disaccharides that have only two linkage bonds, the alpha2-->8-linked disialoside has four: C2-O, O-C8', C8'-C7', and C7'-C6'. The present report describes the results from nine 1 ns MD simulations of alpha2-->8-linked disialoside (Neu5Ac-alpha2-->8-Neu5Ac); simulations were run using GROMOS96 by explicitly considering the solvent molecules. Conformations around the O-C8' bond are restricted to the +sc/+ap regions due to stereochemical reasons. In contrast, conformations around the C2-O and C8'-C7' bonds were found to be largely unrestricted and all the three staggered regions are accessible. The conformations around the C7'-C6' bond were found to be in either the -sc or the anti region. These results are in excellent agreement with the available NMR and potential energy calculation studies. Overall, the disaccharide is flexible and adopts mainly two ensembles of conformations differing in the conformation around the C7'-C6' bond. The flexibility associated with this disaccharide allows for better optimization of intermolecular contacts while binding to proteins and this may partially compensate for the loss of conformational entropy that may be incurred due to disaccharide's flexibility. 相似文献
62.
63.
64.
65.
66.
Rajitha G. Arya C. G. Janardhan B. Laxmi S. V. Ramesh G. Kumari U. Sujana 《Russian Journal of Bioorganic Chemistry》2020,46(4):612-619
Russian Journal of Bioorganic Chemistry - Discovery towards the potent antimicrobial agents is indispensable for the treatment of infections caused by resistant microbes. Thus, we prepared a novel... 相似文献
67.
Sunanda Mallick Ashish Kumar Hiren Dodia Cyrus Alexander Dileep Vasudevan Tushar
Kant Beuria 《Bioscience reports》2021,41(1)
The peptidoglycan (PG) layer is an intricate and dynamic component of the bacterial cell wall, which requires a constant balance between its synthesis and hydrolysis. FtsEX complex present on the inner membrane is shown to transduce signals to induce PG hydrolysis. FtsE has sequence similarity with the nucleotide-binding domains (NBDs) of ABC transporters. The NBDs in most of the ABC transporters couple ATP hydrolysis to transport molecules inside or outside the cell. Also, this reaction cycle is driven by the dimerization of NBDs. Though extensive studies have been carried out on the Escherchia coli FtsEX complex, it remains elusive regarding how FtsEX complex helps in signal transduction or transportation of molecules. Also, very little is known about the biochemical properties and ATPase activities of FtsE. Because of its strong interaction with the membrane-bound protein FtsX, FtsE stays insoluble upon overexpression in E. coli, and thus, most studies on E. coli FtsE (FtsEEc) in the past have used refolded FtsE. Here in the present paper, for the first time, we report the soluble expression, purification, and biochemical characterization of FtsE from E. coli. The purified soluble FtsE exhibits high thermal stability, exhibits ATPase activity and has more than one ATP-binding site. We have also demonstrated a direct interaction between FtsE and the cytoplasmic loop of FtsX. Together, our findings suggest that during bacterial division, the ATPase cycle of FtsE and its interaction with the FtsX cytoplasmic loop may help to regulate the PG hydrolysis at the mid cell. 相似文献
68.
Scindiya M. Malathi P. Kaverinathan K. Sundar A. Ramesh Viswanathan R. 《Molecular biology reports》2021,48(3):2053-2061
Molecular Biology Reports - Red rot caused by Colletotrichum falcatum, is one of the economically important disease of sugarcane and breeding for resistant varieties is considered to be the major... 相似文献
69.
70.
Cluster Computing - Cloud computing model offers various platforms services and provides a scalable, on-demand service at any time-anywhere manner. However, in the outsourcing strategy, users no... 相似文献