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371.
Vassilis Tsafas Konstantina Giouroukou Konstantinos Kounakis Meropi Mari Costas Fotakis Nektarios Tavernarakis George Filippidis 《Journal of biophotonics》2021,14(12):e202100173
The in-vivo elucidation of the molecular mechanisms underlying muscles dysfunction due to aging via non-invasive label free imaging techniques is an important issue with high biological significance. In this study, polarization-dependent second-harmonic generation (PSHG) was used to evaluate structural alterations in the striated muscles during Caenorhabditis elegans lifespan. Young and old wild-type animals were irradiated. The obtained results showed that it was not feasible to detect differences in the structure of myosin that could be correlated with the aging of the worms, via the implementation of the classical, widely used, cylindrical symmetry model and the calculation of the SHG anisotropy values. A trigonal symmetry model improved the extracted results; however, the best outcome was originated from the application of a general model. Myosin structural modifications were monitored via the estimation of the difference in spectral phases derived from discrete Fourier transform analysis. Age classification of the nematodes was achieved by employing both models, proving the usefulness of the usage of PSHG microscopy as a potential diagnostic tool for the investigation of muscle diseases. 相似文献
372.
Richard D. Hayward Peter J. Hume Daniel Humphreys Neil Phillips Katherine Smith Vassilis Koronakis 《Cellular microbiology》2009,11(3):433-441
Enteropathogenic Escherichia coli (EPEC) mimic a ligand–receptor interaction to induce 'pedestal-like' pseudopodia on mammalian cells, providing a tractable system to study tyrosine kinase signalling to the actin cytoskeleton. EPEC delivers its own receptor (Tir), which is engaged by a bacterial surface ligand (intimin). When Tir delivery and activity are uncoupled, intimin-induced Tir clustering stimulates TirY474 phosphorylation by the Src-family kinase (SFK) c-Fyn, triggering actin polymerization and pedestal formation. How c-Fyn specifically targets Tir and is regulated remains unknown. We show that clustering transfers Tir into cholesterol-rich detergent-resistant microdomains (DRMs), a signal prompting transient c-Fyn accumulation at bacterial adhesion sites. Co-clustering of TirY474 and c-Fyn in DRMs rapidly stimulates robust kinase activation both by induced c-FynY531 dephosphorylation to unlock the inactive state and by reciprocal c-FynY417 autophosphorylation to promote activity. After signal induction, c-Fyn dissipates and the resting state restored by Csk-dependent phosphorylation of c-FynY531 . These data illustrate a sophisticated mechanism evolved by a pathogen effector to reversibly regulate SFKs, and resolve early interactions at a model receptor initiating tyrosine kinase signalling. 相似文献
373.
Vassilis J. Marmaras Nektarios Charalambidis 《Archives of insect biochemistry and physiology》1992,21(4):281-288
The results indicate that certain hemocyte proteins of the medfly, Ceratitis capitata, are responsible for the recognition of foreignness, since they are able to bind to the surface of Escherichia coli in vitro. Furthermore, when the E. Coli-hemocyte protein complex was incubated in the presence of tyrosine and phenoloxidase, the bacteria were immobilized, forming large aggregates. The formation of aggregates seems to be due to reactive tyrosine intermediate (quinone) generated by the action of phenoloxidase. © 1992 Wiley-Liss, Inc. 相似文献
374.
Summary Since 1859, wintering Lesser White-fronted Geese have been recorded 25 times in Greece. Of the records obtained in the second half of the 19th century only two, based on museum specimens, are valid. There were four records in the first half of the present century, while all the others come from the second half due to increased coverage mostly during IWRB midwinter censuses. Their distribution had been more southerly in the past, but in recent years only northern Greek wetlands hold the bulk of the wintering population, with the Evros Delta being the most important area (12, or 48% of all records). The wintering population of the species in the Evros Delta is threatened by habitat destruction and shooting.
Zum Vorkommen der Zwerggans in Griechenland
Zusammenfassung Seit 1859 wurden in Griechenland 25mal Zwerggänse festgestellt; aus dem 19. Jh. liegen nur 2 sichere Nachweise vor. 4 Nachweise fallen in die erste Hälfte des 20. Jh., die restlichen alle in die zweite Hälfte, Die Verteilung der Funde scheint in der Vergangenheit weiter nach Süden gereicht zu haben; heute liegen nur aus dem Norden Beobachtungen vor. Der wichtigste Platz ist das Evros-Delta (12 von 25 Nachweisen; einmal 1630 Ind. im Februar 1963). Die Winterpopulation ist durch Biotopzerstörung und Abschuß bedroht.相似文献
375.
Vasiliki Lykopoulou Vassilis Karageorgiou Chrisi Vasiliadou Christos Ritzoulis 《Food biophysics》2017,12(4):433-438
The events occurring during the spontaneous mixing of an okra mucilage-based model liquid food with saliva were recorded, with a focus on the local dynamics of colloidal particles contained therein. An okra mucilage containing fluorescent microsphere probes (assuming the role of model emulsion droplets), was brought into contact with reconstituted saliva on the welled slide of a confocal microscope. The movement of the probes at the saliva–mucilage interface and in the bulk mucilage has been recorded over periods of 60 min. Data have been obtained on the total mobility of individual model droplets, their diffusion coefficients, and the local viscosities, providing information on the dynamics of processes which occur during the oral processing of colloidal liquids. The role of osmotic pressure gradients on the spreading of the droplets into saliva is discussed in relation to the microrheology data. 相似文献
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