全文获取类型
收费全文 | 159篇 |
免费 | 8篇 |
出版年
2021年 | 3篇 |
2019年 | 1篇 |
2018年 | 2篇 |
2017年 | 4篇 |
2016年 | 6篇 |
2015年 | 8篇 |
2014年 | 7篇 |
2013年 | 8篇 |
2012年 | 13篇 |
2011年 | 19篇 |
2010年 | 11篇 |
2009年 | 6篇 |
2008年 | 7篇 |
2007年 | 9篇 |
2006年 | 7篇 |
2005年 | 10篇 |
2004年 | 10篇 |
2003年 | 9篇 |
2002年 | 7篇 |
2001年 | 2篇 |
2000年 | 1篇 |
1999年 | 2篇 |
1998年 | 1篇 |
1997年 | 2篇 |
1994年 | 1篇 |
1991年 | 1篇 |
1986年 | 1篇 |
1982年 | 1篇 |
1976年 | 1篇 |
1973年 | 1篇 |
1970年 | 1篇 |
1967年 | 1篇 |
1963年 | 1篇 |
1961年 | 3篇 |
排序方式: 共有167条查询结果,搜索用时 78 毫秒
61.
Mrakovcic-Sutić I Jakovac H Simin M Grebić D Cuk M Trobonjaca Z Radosevic-Stasić B 《Histology and histopathology》2008,23(9):1111-1126
Tissue disintegration after injury leads, in the endoplasmic reticulum (ER), to activation of adaptive pathways known as the ER stress response. It is directed to the correction of unfolded proteins and to the activation of proteasome-dependent ER-associated degradation of the misfolded proteins, but induces also a rapid activation of natural and adaptive immunity, since a ER resident heat shock protein-gp96 acts not only as a molecular chaperone, but also as a strong adjuvant, able to cross-present the antigenic peptides onto MHC class I or MHC class II pathways. Analyzing its potential role in processes of normal growth, in mice subjected to 1/3 partial hepatectomy (pHx) we determined the tissue expression of gp96 protein and mRNA in regenerating liver, thymus and spleen, determining simultaneously the phenotypic profile and spontaneous cytotoxic activity of intrahepatic and splenic mononuclear lymphatic cells (MNLC) against NKT- and NK-cells sensitive targets (syngeneic thymocytes and YAC-1) in wild, perforin and FasL deficient mice. The data have shown that pHx induces fast overexpression of gp96 protein and mRNA in hepatocytes, spleen and thymus, with accumulation of CD3intermediate/NK1.1+/CD69+ cells (liver) and Foxp3+CD4+CD25+ cells (liver and thymus). Simultaneously, intrahepatic MNLC acquired the FasL-dependent cytotoxic potential against NKT-sensitive targets and both, intrahepatic and splenic MNLC, acquired the perforin-dependent cytotoxic potential against NK-sensitive targets, implying that during the disturbance of morphostasis gp96 serves as a natural adjuvant for chaperoning antigenic self peptides into the immune surveillance pathways, resulting in activation of autoreactive NKT and regulatory cells, as well as NK cells. Moreover, cell cycle analysis revealed that G2+M phase of regenerating hepatocytes in PKO mice was translocated from the 1st to the 7th p. o. day, as well as that hepatocytes from FasL deficient mice were arrested in G0/G1 phase. 相似文献
62.
Canola (Brassica napus L.) cultivars with improved nitrogen utilization efficiency (NUE) at grain harvest are of interest to growers to reduce fertilizer
inputs. Our objective was to determine whether cultivar-specific responses in NUE (seed yield per N accumulated in the whole
plant) could be related to the differences in dry matter and N partitioning among various plant parts. Four spring canola
cultivars were grown in a glasshouse under the conditions of low and high N supply. When compared to high-N treatment, deficient
N conditions resulted in a similar decrease in dry weight for all cultivars, averaging 46% for shoot, 47% for root, and 45%
for dropped leaves. The reductions in N concentrations at low-N compared to high-N treatment were much smaller and averaged
15% for shoot, 16% for root and 10% for dropped leaves. Although significant variations occurred for dry weight, N concentration
and N uptake in various plant sections, all cultivars accumulated a similar amount of N in total plant biomass at harvest.
However, significant differences in plant biomass, seed yield and consequently, NUE existed because more N-efficient cultivars
Eyre and Charlton produced larger seed yields than less N-efficient cultivars Pinnacle and Rainbow. No consistent variations
in N concentration in various plant parts could be established among tested cultivars. Thus, cultivar-specific responses in
NUE were mainly attributed to the differences in the root-to-shoot ratio and harvest index. N-efficient Eyre produced seed
yield similar to the highest yielding Charlton, though it had the smallest plant dry weight of all cultivars. In contrast,
N-inefficient Rainbow had the largest plant biomass, but produced the smallest seed yield because of its lowest harvest index
and the highest root-to-shoot ratio. The absence of cultivar×N treatment interactions indicated that cultivars performed similarly
for plant biomass, N uptake and seed yield across two contrasting N supplies. Canola cultivars significantly differed in NUE
despite a similar amount of absorbed N in plant biomass; more N-efficient cultivars outyielded less N-efficient ones primarily
because of cultivar-specific variations in the root-to-shoot ratio and harvest index. 相似文献
63.
64.
Čedomila Milin Robert Domitrović Marin Tota Jasminka Giacometti Mira Ćuk Biserka Radošević-Stašić Zlatko Ciganj 《Biological trace element research》2001,82(1-3):201-210
The mineral content (zinc, iron, magnesium, and calcium) in the liver, spleen, and thymus of male Balb/C mice was analyzed.
Animals were fed, over 21 d, diets enriched with corn oil (FCO diet) or olive oil (FOO diet) (5% addition to standard pellet,
w/w). Olive oil with predominant oleic acid (C18:1, n-9) had a quite different composition than corn oil, in which linoleic
acid (C18:2, n-6) prevails. The zinc and magnesium tissue concentrations were not changed in either group. The calcium concentration
in liver as well as the calcium concentration in spleen increased in mice fed both the FCO and FOO diets. Furthermore, mice
fed both the FOO and FCO diets had increased spleen iron concentration. Mice fed the FCO diet had increased thymus calcium
concentration compared to controls. The results show the effect of diets with unsaturated, particularly polyunsaturated fatty
acids, on the calcium and iron concentration in some organs. 相似文献
65.
Previous work in this laboratory showed that diethylstilbestrol was capable of suppressing induced furunculosis in rabbits. The present study indicates that the synthetic estrogenic hormone diethylstilbestrol which is used for acne, estrogen deficiency, cancer, and other disorders, can reduce the cytolytic action of staphylococcal alpha toxin. The cytotoxic action of purified alpha toxin for tissue cultures was evaluated by use of such parameters as total and viable cell counts, glucose, and protein determination, and cytopathic effects (CPE) in the presence and absence of steroids. To 3-day-old primary rabbit baby kidney tissue cultures, 1 to 5 μg of diethylstilbestrol per ml was added; growth of tissue cultures in Eagles medium was continued till the 6th day, and then one tissue cytopathic dose per milliliter of alpha toxin was added, and the subsequent fate of tissue cultures was assayed. Such cultures yielded higher total and viable cell counts, utilized more glucose, and contained more protein than the control cultures. In control cultures, CPE was observed on the 3rd hr after the addition of alpha toxin, and it was complete in 24 hr, whereas in tissue cultures treated with diethylstilbestrol, the CPE was significantly reduced. The data presented in this study made possible the availability of a suppressor of the cytolytic action of alpha toxin and might be useful in assaying the action of alpha toxin in an in vitro inexpensive test system. 相似文献
66.
Ross SA ElSohly MA Sultana GN Mehmedic Z Hossain CF Chandra S 《Phytochemical analysis : PCA》2005,16(1):45-48
Chemical investigation of the pollen grain collected from male plants of Cannabis sativa L. resulted in the isolation for the first time of two flavonol glycosides from the methanol extract, and the identification of 16 cannabinoids in the hexane extract. The two glycosides were identified as kaempferol 3-O-sophoroside and quercetin 3-O-sophoroside by spectroscopic methods including high-field two-dimensional NMR experiments. The characterisation of each cannabinoid was performed by GC-FID and GC-MS analyses and by comparison with both available reference cannabinoids and reported data. The identified cannabinoids were delta9-tetrahydrocannabiorcol, cannabidivarin, cannabicitran, delta9-tetrahydrocannabivarin, cannabicyclol, cannabidiol, cannabichromene, delta9-tetrahydrocannabinol, cannabigerol, cannabinol, dihydrocannabinol, cannabielsoin, 6a, 7, 10a-trihydroxytetrahydrocannabinol, 9, 10-epoxycannabitriol, 10-O-ethylcannabitriol, and 7, 8-dehydro-10-O-ethylcannabitriol. 相似文献
67.
68.
Bettina Schlick Petra Massoner Angelika Lueking Pornpimol Charoentong Mirjam Blattner Georg Schaefer Klaus Marquart Carmen Theek Peter Amersdorfer Dirk Zielinski Matthias Kirchner Zlatko Trajanoski Mark A. Rubin Stefan Müllner Peter Schulz-Knappe Helmut Klocker 《PloS one》2016,11(2)
Background
Chronic inflammation is frequently observed on histological analysis of malignant and non-malignant prostate specimens. It is a suspected supporting factor for prostate diseases and their progression and a main cause of false positive PSA tests in cancer screening. We hypothesized that inflammation induces autoantibodies, which may be useful biomarkers. We aimed to identify and validate prostate inflammation associated serum autoantibodies in prostate cancer patients and evaluate the expression of corresponding autoantigens.Methods
Radical prostatectomy specimens of prostate cancer patients (N = 70) were classified into high and low inflammation groups according to the amount of tissue infiltrating lymphocytes. The corresponding pre-surgery blood serum samples were scrutinized for autoantibodies using a low-density protein array. Selected autoantigens were identified in prostate tissue and their expression pattern analyzed by immunohistochemistry and qPCR. The identified autoantibody profile was cross-checked in an independent sample set (N = 63) using the Luminex-bead protein array technology.Results
Protein array screening identified 165 autoantibodies differentially abundant in the serum of high compared to low inflammation patients. The expression pattern of three corresponding antigens were established in benign and cancer tissue by immunohistochemistry and qPCR: SPAST (Spastin), STX18 (Syntaxin 18) and SPOP (speckle-type POZ protein). Of these, SPAST was significantly increased in prostate tissue with high inflammation. All three autoantigens were differentially expressed in primary and/or castration resistant prostate tumors when analyzed in an inflammation-independent tissue microarray. Cross-validation of the inflammation autoantibody profile on an independent sample set using a Luminex-bead protein array, retrieved 51 of the significantly discriminating autoantibodies. Three autoantibodies were significantly upregulated in both screens, MUT, RAB11B and CSRP2 (p>0.05), two, SPOP and ZNF671, close to statistical significance (p = 0.051 and 0.076).Conclusions
We provide evidence of an inflammation-specific autoantibody profile and confirm the expression of corresponding autoantigens in prostate tissue. This supports evaluation of autoantibodies as non-invasive markers for prostate inflammation. 相似文献69.
Chemotype Diversity of Indigenous Dalmatian Sage (Salvia officinalis L.) Populations in Montenegro
下载免费PDF全文
![点击此处可从《化学与生物多样性》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Danijela Stešević Mihailo Ristić Vuko Nikolić Marijana Nedović Danka Caković Zlatko Šatović 《化学与生物多样性》2014,11(1):101-114
To identify how many chemotypes of Salvia officinalis exist in Montenegro, the chemical composition of the essential oils of 12 wild‐growing populations was determined by GC‐FID and GC/MS analyses. Among the 40 identified constituents, the most abundant were cis‐thujone (16.98–40.35%), camphor (12.75–35.37%), 1,8‐cineol (6.40–12.06%), trans‐thujone (1.5–10.35%), camphene (2.26–9.97%), borneol (0.97–8.81%), viridiflorol (3.46–7.8%), limonene (1.8–6.47%), α‐pinene (1.59–5.46%), and α‐humulene (1.77–5.02%). The composition of the essential oils under study did not meet the ISO 9909 requirements, while the oils of populations P02–P04, P09, and P10 complied with the German Drug Codex. A few of the main essential‐oil constituents appeared to be highly intercorrelated. Strong positive correlations were observed between α‐pinene and camphene, camphene and camphor, as well as between cis‐thujone and trans‐thujone. Strong negative correlations were evidenced between cis‐thujone and α‐pinene, cis‐thujone and champhene, cis‐thujone and camphor, as well as between trans‐thujone and camphene. Multivariate analyses allowed the grouping of the populations into three distinct chemotypes, i.e., Chemotype A, rich in total thujones, Chemotype B, with intermediate contents of thujones, α‐pinene, camphene, and camphor and high borneol contents, and Chemotype C, rich in camphor, camphene, and α‐pinene. The chemotypes did not significantly differ in the total essential‐oil content and the cis/trans‐thujone ratio. 相似文献
70.
Identification of small gains and losses in single cells after whole genome amplification on tiling oligo arrays
下载免费PDF全文
![点击此处可从《Nucleic acids research》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Jochen B. Geigl Anna C. Obenauf Julie Waldispuehl-Geigl Eva M. Hoffmann Martina Auer Martina H?rmann Maria Fischer Zlatko Trajanoski Michael A. Schenk Lars O. Baumbusch Michael R. Speicher 《Nucleic acids research》2009,37(15):e105
Clinical DNA is often available in limited quantities requiring whole-genome amplification for subsequent genome-wide assessment of copy-number variation (CNV) by array-CGH. In pre-implantation diagnosis and analysis of micrometastases, even merely single cells are available for analysis. However, procedures allowing high-resolution analyses of CNVs from single cells well below resolution limits of conventional cytogenetics are lacking. Here, we applied amplification products of single cells and of cell pools (5 or 10 cells) from patients with developmental delay, cancer cell lines and polar bodies to various oligo tiling array platforms with a median probe spacing as high as 65 bp. Our high-resolution analyses reveal that the low amounts of template DNA do not result in a completely unbiased whole genome amplification but that stochastic amplification artifacts, which become more obvious on array platforms with tiling path resolution, cause significant noise. We implemented a new evaluation algorithm specifically for the identification of small gains and losses in such very noisy ratio profiles. Our data suggest that when assessed with sufficiently sensitive methods high-resolution oligo-arrays allow a reliable identification of CNVs as small as 500 kb in cell pools (5 or 10 cells), and of 2.6–3.0 Mb in single cells. 相似文献